LOCUS BC032505 1239 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens proenkephalin, mRNA (cDNA clone MGC:45212
IMAGE:5244387), complete cds.
ACCESSION BC032505
VERSION BC032505.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1239)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1239)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: c Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 40254835.
FEATURES Location/Qualifiers
source 1..1239
/db_xref="H-InvDB:HIT000041575"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45212 IMAGE:5244387"
/tissue_type="Brain, fetal, whole pooled"
/clone_lib="NIH_MGC_121"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1239
/gene="PENK"
/db_xref="GeneID:5179"
/db_xref="HGNC:HGNC:8831"
/db_xref="MIM:131330"
CDS 77..880
/gene="PENK"
/codon_start=1
/product="proenkephalin"
/protein_id="AAH32505.1"
/db_xref="GeneID:5179"
/db_xref="HGNC:HGNC:8831"
/db_xref="MIM:131330"
/translation="MARFLTLCTWLLLLGPGLLATVRAECSQDCATCSYRLVRPADIN
FLACVMECEGKLPSLKIWETCKELLQLSKPELPQDGTSTLRENSKPEESHLLAKRYGG
FMKRYGGFMKKMDELYPMEPEEEANGSEILAKRYGGFMKKDAEEDDSLANSSDLLKEL
LETGDNRERSHHQDGSDNEEEVSKRYGGFMRGLKRSPQLEDEAKELQKRYGGFMRRVG
RPEWWMDYQKRYGGFLKRFAEALPSDEEGESYSKEVPEMEKRYGGFMRF"
BASE COUNT 360 a 278 c 320 g 281 t
ORIGIN
1 cggcgagggt cctgccgagg gacccgttct gcgcccaggc aggctcgaag cacgcgtccc
61 tctctcctcg cagtccatgg cgcggttcct gacactttgc acttggctgc tgttgctcgg
121 ccccgggctc ctggcgaccg tgcgggccga atgcagccag gattgcgcga cgtgcagcta
181 ccgcctagtg cgcccggccg acatcaactt cctggcttgc gtaatggaat gtgaaggtaa
241 actgccttct ctgaaaattt gggaaacctg caaggagctc ctgcagctgt ccaaaccaga
301 gcttcctcaa gatggcacca gcaccctcag agaaaatagc aaaccggaag aaagccattt
361 gctagccaaa aggtatgggg gcttcatgaa aaggtatgga ggcttcatga agaaaatgga
421 tgagctttat cccatggagc cagaagaaga ggccaatgga agtgagatcc tcgccaagcg
481 gtatgggggc ttcatgaaga aggatgcaga ggaggacgac tcgctggcca attcctcaga
541 cctgctaaaa gagcttctgg aaacagggga caaccgagag cgtagccacc accaggatgg
601 cagtgataat gaggaagaag tgagcaagag atatgggggc ttcatgagag gcttaaagag
661 aagcccccaa ctggaagatg aagccaaaga gctgcagaag cgatatgggg gcttcatgag
721 aagagtaggt cgcccagagt ggtggatgga ctaccagaaa cggtatggag gtttcctgaa
781 gcgctttgcc gaggctctgc cctccgacga agaaggcgaa agttactcca aagaagttcc
841 tgaaatggaa aaaagatacg gaggatttat gagattttaa tatttttccc actagtggcc
901 ccaggcccca gcaagcctcc ctccatcctc cagtgggaaa ctgttgatgg tgttttattg
961 tcatgtgttg cttgccttgt atagttgact tcattgtctg gataactata caacctgaaa
1021 actgtcattt caggttctgt gctctttttg gagtctttaa gctcagtatt agtctattgc
1081 agctatctcg ttttcatgct aaaatagttt ttgttatctt gtctcttatt tttgacaaac
1141 atcaataaat gcttacttgt atatagagat aataaaccta ttaccccaag tgcaaaaaaa
1201 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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