LOCUS BC032110 1161 bp mRNA linear HUM 23-DEC-2003
DEFINITION Homo sapiens phosphoglycerate dehydrogenase, mRNA (cDNA clone
IMAGE:4662199), partial cds.
ACCESSION BC032110
VERSION BC032110.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1161)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1161)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 19, 2003 this sequence version replaced BC032110.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 33 Row: j Column: 18.
FEATURES Location/Qualifiers
source 1..1161
/db_xref="H-InvDB:HIT000041423"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4662199"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_18"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..1161
/gene="PHGDH"
/gene_synonym="MGC3017"
/gene_synonym="PDG"
/gene_synonym="PGAD"
/gene_synonym="PGD"
/gene_synonym="PGDH"
/gene_synonym="SERA"
/db_xref="GeneID:26227"
/db_xref="MIM:606879"
CDS <1..970
/gene="PHGDH"
/gene_synonym="MGC3017"
/gene_synonym="PDG"
/gene_synonym="PGAD"
/gene_synonym="PGD"
/gene_synonym="PGDH"
/gene_synonym="SERA"
/codon_start=2
/product="PHGDH protein"
/protein_id="AAH32110.3"
/db_xref="GeneID:26227"
/db_xref="MIM:606879"
/translation="STTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCA
GAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLT
GVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLS
PAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAG
APYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAE
AGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF"
misc_feature 2..217
/gene="PHGDH"
/gene_synonym="MGC3017"
/gene_synonym="PDG"
/gene_synonym="PGAD"
/gene_synonym="PGD"
/gene_synonym="PGDH"
/gene_synonym="SERA"
/note="2-Hacid_DH_C; Region: D-isomer specific
2-hydroxyacid dehydrogenase, NAD binding domain. This
domain is inserted into the catalytic domain, the large
dehydrogenase and D-lactate dehydrogenase families in
SCOP. N-terminal portion of which is represented by family
pfam00389"
/db_xref="CDD:pfam02826"
BASE COUNT 250 a 341 c 339 g 231 t
ORIGIN
1 ctccacgaca ggcttgctga atgacaacac ctttgcccag tgcaagaagg gggtgcgtgt
61 ggtgaactgt gcccgtggag ggatcgtgga cgaaggcgcc ctgctccggg ccctgcagtc
121 tggccagtgt gccggggctg cactggacgt gtttacggaa gagccgccac gggaccgggc
181 cttggtggac catgagaatg tcatcagctg tccccacctg ggtgccagca ccaaggaggc
241 tcagagccgc tgtggggagg aaattgctgt tcagttcgtg gacatggtga aggggaaatc
301 tctcacgggg gttgtgaatg cccaggccct taccagtgcc ttctctccac acaccaagcc
361 ttggattggt ctggcagaag ctctggggac actgatgcga gcctgggctg ggtcccccaa
421 agggaccatc caggtgataa cacagggaac atccctgaag aatgctggga actgcctaag
481 ccccgcagtc attgtcggcc tcctgaaaga ggcttccaag caggcggatg tgaacttggt
541 gaacgctaag ctgctggtga aagaggctgg cctcaatgtc accacctccc acagccctgc
601 tgcaccaggg gagcaaggct tcggggaatg cctcctggcc gtggccctgg caggcgcccc
661 ttaccaggct gtgggcttgg tccaaggcac tacacctgta ctgcaggggc tcaatggagc
721 tgtcttcagg ccagaagtgc ctctccgcag ggacctgccc ctgctcctat tccggactca
781 gacctctgac cctgcaatgc tgcctaccat gattggcctc ctggcagagg caggcgtgcg
841 gctgctgtcc taccagactt cactggtgtc agatggggag acctggcacg tcatgggcat
901 ctcctccttg ctgcccagcc tggaagcgtg gaagcagcat gtgactgaag ccttccagtt
961 ccacttctaa ccttggagct cactggtccc tgcctctggg gcttttctga agaaacccac
1021 ccactgtgat caatagggag agaaaatcca cattcttggg ctgaacgcgg gcctctgaca
1081 ctgcttacac tgcactctga ccctgtagta cagcaataac cgtctaataa agagcctacc
1141 cccaaaaaaa aaaaaaaaaa a
//