LOCUS BC031573 3177 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens neuronal guanine nucleotide exchange factor, mRNA
(cDNA clone MGC:35393 IMAGE:5186132), complete cds.
ACCESSION BC031573
VERSION BC031573.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3177)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3177)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 50 Row: f Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 29789101.
FEATURES Location/Qualifiers
source 1..3177
/db_xref="H-InvDB:HIT000041317"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:35393 IMAGE:5186132"
/tissue_type="Colon, Kidney, Stomach, adult, whole pooled"
/clone_lib="NIH_MGC_116"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3177
/gene="NGEF"
/gene_synonym="EPHEXIN"
/db_xref="GeneID:25791"
/db_xref="HGNC:HGNC:7807"
/db_xref="MIM:605991"
CDS 236..2368
/gene="NGEF"
/gene_synonym="EPHEXIN"
/codon_start=1
/product="neuronal guanine nucleotide exchange factor"
/protein_id="AAH31573.1"
/db_xref="GeneID:25791"
/db_xref="HGNC:HGNC:7807"
/db_xref="MIM:605991"
/translation="METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQA
DQDIQDKEPHCHIPIKRNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPL
TLNIPWSRTPPCRTAMQTDPGAQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMI
HPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEE
EEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQE
AMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLE
LEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIA
QLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMV
VKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTL
RTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFIL
RLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYV
AQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLK
ECFRVHKMDDPQRSQNKDRRKLGSRNRQ"
BASE COUNT 795 a 911 c 891 g 580 t
ORIGIN
1 caggacgaag tttttggctg tgggctctct gggacagagt ggagcgcctg tggatttagc
61 cggggacaca ccatcctgct cgcaggtgcc tggacaggac aggggacgag ggaggaacac
121 tctctgagga agcccaactc ctatgagctc ctctctggca gcactcaaac cagtgaagct
181 tgggaaagtc attgacctcc agtcgttctg ctgagaaaca tctggctcta tttccatgga
241 gaccagggaa tctgaagatt tggaaaagac ccggaggaaa tcagcaagtg atcaatggaa
301 cactgataat gaaccagcca aggtgaaacc tgagttactc ccagaaaaag aggagacttc
361 tcaagctgac caggatatcc aagacaaaga gcctcattgc cacatcccaa ttaagagaaa
421 ttccatcttc aatcgctcca taagacgcaa aagcaaagcc aaggccagag acaaccccga
481 acggaacgcc agctgcctgg cagattcaca ggacaatgga aaatctgtaa atgagcccct
541 gaccttgaat atcccctgga gcagaacgcc tccttgcaga acagcaatgc agacagaccc
601 aggagcccag gaaatgagtg agtcgtcctc caccccggga aatggggcca cgcccgagga
661 gtggccggcc ctggccgaca gccccaccac gctcaccgag gccctgcgga tgatccaccc
721 cattcccgcc gactcctgga gaaacctcat tgaacaaata gggctcctgt atcaggaata
781 ccgagataaa tcgactctcc aagaaatcga aaccaggagg caacaggatg cagaaataga
841 agacaatacc aatgggtccc cggccagtga ggacaccccg gaggaggaag aagaagagga
901 ggaggaggag gagccggcca gcccaccaga gaggaagact ctgccccaga tctgcctgct
961 cagtaacccc cactcaaggt tcaacctctg gcaggatctt cccgagatcc ggagcagcgg
1021 ggtgcttgag atcctacagc ctgaggagat taagctgcag gaggccatgt tcgagctggt
1081 cacttccgag gcgtcctact acaagagtct gaacctgctc gtgtcccact tcatggagaa
1141 cgagcggata aggaagatcc tgcacccgtc cgaggcgcac atcctcttct ccaacgtcct
1201 ggacgtgctg gctgtcagtg agcggttcct cctggagctg gagcaccgga tggaggagaa
1261 catcgtcatc tctgacgtgt gtgacatcgt gtaccgttat gcggctgacc acttctctgt
1321 ctacatcacc tacgtcagca atcagaccta ccaggagcgg acctataagc agctgctcca
1381 ggagaaggca gctttccggg agctgatcgc gcagctagag ctcgacccca agtgcagggg
1441 gctgcccttc tcctccttcc tcatcctgcc tttccagagg atcacacgcc tcaagctgtt
1501 ggtccagaac atcctgaaga gggtagaaga gaggtctgag cgggagtgca ctgctttgga
1561 tgctcacaag gagctggaaa tggtggtgaa ggcatgcaac gagggcgtca ggaaaatgag
1621 ccgcacggaa cagatgatca gcattcagaa gaagatggag ttcaagatca agtcggtgcc
1681 catcatctcc cactcccgct ggctgctgaa gcagggtgag ctgcagcaga tgtcaggccc
1741 caagacctcc cggaccctga ggaccaagaa gctcttccac gaaatttacc tcttcctgtt
1801 caacgacctg ctggtgatct gccggcagat tccaggagac aagtaccagg tatttgactc
1861 agctccgcgg ggactgctgc gtgtggagga gctggaggac cagggccaga cgctggccaa
1921 cgtgttcatc ctgcggctgc tggagaacgc agatgaccgg gaggccacct acatgctaaa
1981 ggcgtcctct cagagtgaga tgaagcgttg gatgacctca ctggccccca acaggaggac
2041 caagtttgtt tcgttcacat cccggctgct ggactgcccc caggtccagt gcgtgcaccc
2101 atacgtggct cagcagccag acgagctgac gctggagctc gccgacatcc tcaacatcct
2161 ggacaagact gacgacgggt ggatctttgg cgagcgtctg cacgaccagg agagaggctg
2221 gttccccagc tccatgactg aggagatctt gaatcccaag atccggtccc agaacctcaa
2281 ggaatgtttc cgtgtccaca agatggatga ccctcagcgc agccagaaca aggaccgcag
2341 gaagctgggc agccggaatc ggcaatgacc cccacccagg gggccagcgg gagcagggcc
2401 tgcatgagac cccgacagaa ggtggggggg gggcctctgg gaagcacagg ccagcacctc
2461 cccaggtggc aggatctggc ttggggtgcc cggccctcat ccctgcccac gcagtgagtg
2521 ctcatgtgtc ttggcccctt gcttgcaaac tggataaagg gtgcccaagc ctctcctgat
2581 gcatttgtaa acaagaaggt ttcagcagta ttacaccacc tccctcatgc ctccgagggg
2641 gtggaagggg gtgggcacac tccagggccc cccatgcccc tggcccccag ggactggaag
2701 aggctcccaa cccagagtgt ccctgtggga ggcaggcaga aggtgacaat tgacacgatt
2761 tcctgcacgc gtcctcctct accttggaag cagttagaat ctaccaggca cagatgaggc
2821 cgcccttgcc tgacggagct tgatgagcag cccttggtct ccggttccag gactgagagc
2881 ccagctgcct ctgcccaccc ttccccaggc ctctgccagc ctctggctgc acggtcaggc
2941 cctgccccat ggcaggcctg ccagagcttg gctggggacc cctcccgcct ctggctccct
3001 gatgggctgg atgtaacttg tgtcttctag ccgcttaagg agcccaggtg ttttaaggaa
3061 tgaattggtc actgcatctt gtatcgatta tggttctgag aaaagcaaat atcacttttg
3121 gctgcattaa aagaagcatc atatataaaa taaaaaaaaa aaaaaaaaaa aaaaaaa
//