LOCUS BC030691 4823 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens sparc/osteonectin, cwcv and kazal-like domains
proteoglycan (testican) 1, mRNA (cDNA clone MGC:26308
IMAGE:4821322), complete cds.
ACCESSION BC030691
VERSION BC030691.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4823)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4823)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (24-MAY-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC030691.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 33 Row: b Column: 20
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 15451924.
FEATURES Location/Qualifiers
source 1..4823
/db_xref="H-InvDB:HIT000041102"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:26308 IMAGE:4821322"
/tissue_type="Brain, hippocampus"
/clone_lib="NIH_MGC_95"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4823
/gene="SPOCK1"
/gene_synonym="TESTICAN"
/db_xref="GeneID:6695"
/db_xref="HGNC:HGNC:11251"
/db_xref="MIM:602264"
CDS 133..1452
/gene="SPOCK1"
/gene_synonym="TESTICAN"
/codon_start=1
/product="sparc/osteonectin, cwcv and kazal-like domains
proteoglycan (testican) 1"
/protein_id="AAH30691.1"
/db_xref="GeneID:6695"
/db_xref="HGNC:HGNC:11251"
/db_xref="MIM:602264"
/translation="MPAIAVLAAAAAAWCFLQVESRHLDALAGGAGPNHGNFLDNDQW
LSTVSQYDRDKYWNRFRDDDYFRNWNPNKPFDKALDPSKDPCLKVKCSPHKVCVTQDY
QTALCVSRKHLLPRQKKGNVAQKHWVGPSNLVKCKPCPVAQSAMVCGSDGHSYTSKCK
LEFHACSTGKSLATLCDGPCPCLPEPEPPKHKAERSACTDKELRNLASRLKDWFGALH
EDANRVIKPTSSNTAQGRFDTSILPICKDSLGWMFNKLDMNYDLLLDPSEINAIYLDK
YEPCIKPLFNSCDSFKDGKLSNNEWCYCFQKPGGLPCQNEMNRIQKLSKGKSLLGAFI
PRCNEEGYYKATQCHGSTGQCWCVDKYGNELAGSRKQGAVSCEEEQETSGDFGSGGSV
VLLDDLEYERELGPKDKEGKLRVHTRAVTEDDEDEDDDKEDEVGYIW"
BASE COUNT 1349 a 1137 c 989 g 1348 t
ORIGIN
1 acggagcggc cagacgctcg gcggcggcgt gtggcaggag cgcaggggcg cgagccggcg
61 atcagccttc ccggcgaccg tgccgcggga gctcgagcaa ctcggactag gggacccggg
121 ccggccccca agatgccggc gatcgcggtg ttggcggcgg ccgccgcggc gtggtgcttc
181 ctccaagtcg agagccggca cctggacgcg ctcgccggag gcgcgggccc caaccacggc
241 aatttcctag acaatgacca gtggctgagc accgtctccc agtacgaccg ggacaagtac
301 tggaaccgct ttcgagacga tgattatttc agaaactgga atcccaacaa gccctttgac
361 aaagccctgg acccatccaa ggacccctgc ctgaaggtaa aatgcagccc tcacaaagtg
421 tgtgtgaccc aggactacca gaccgccctg tgtgtcagcc gcaagcacct gctccccagg
481 caaaagaagg ggaacgtggc ccagaaacac tgggttggac cttcgaattt ggtcaagtgc
541 aagccctgtc ccgtggcaca gtcagccatg gtctgcggct cagatggcca ctcctacaca
601 tccaagtgca aattggagtt ccatgcttgt tctactggca aaagcctcgc caccctctgt
661 gatgggccct gtccctgtct cccagagcct gagccaccaa agcacaaggc agaaaggagt
721 gcctgcacag acaaggagtt gcggaacctt gcctcccggc tgaaggattg gtttggagct
781 ctccacgagg atgcgaacag agtcatcaag cccaccagct ccaacacagc ccaaggcagg
841 tttgacacta gcatcctgcc catctgcaag gactccctgg gctggatgtt caacaagttg
901 gacatgaact atgacctcct gcttgaccct tcagagatca atgccatcta cctggataag
961 tacgagccct gtatcaagcc tcttttcaac tcgtgtgact ccttcaagga tggcaagctt
1021 tctaacaatg agtggtgcta ctgcttccag aagcctggag gtctcccttg ccagaatgaa
1081 atgaacagaa ttcagaagct gagtaagggg aaaagcctgt tgggggcctt catacctcgg
1141 tgtaatgagg agggctatta caaagccaca cagtgccacg gcagcacggg gcagtgctgg
1201 tgtgtggaca aatatgggaa tgagttggct ggctccagga aacagggtgc tgtgagctgt
1261 gaagaggagc aggaaacctc aggggatttt ggcagtggtg ggtccgtggt cctgctggat
1321 gacctagaat atgaacggga gctgggacca aaggacaaag aggggaagct gagggtgcac
1381 acccgagccg tgacagagga tgatgaggat gaggatgatg acaaagagga tgaggtcggg
1441 tacatatggt agtgcccaca agaaagagga cacaagtttt gcacaaaatt gcaagtcact
1501 tcctattcct gcatttgtat ctaagactcc aaggcaccaa ggtctcttct ccattgttgc
1561 tctctatacc cgacctaagg tttggaagac aactgcttgt tcccagagga ttctgatttt
1621 gcatatgttt gtatgggaga aagggtgttg tgtttttttt tttgttgttg tttatttttt
1681 ggatagggaa gtcattggct taattagagc ctccttcctt tctgtgagat ttttccaaca
1741 agcatgtgat ttacgtggaa ttctgacagt gcagggagcc cccaccctct taaatgtcaa
1801 agaccctttt tgattaccca cactggtggt tattacagca tggttcccag ccttacagtg
1861 tctaagtgct tctcttgtgt cctgtagatg ttgtgaaaaa gaaaaaaaca aaaaatacac
1921 cacactgtac tttttccccc tgcccccgtt actgccggtg attattatta aaaattagtt
1981 tttttcacat cattatatct ggcttcctat aaacaacagc cttaattcag tcaagactcc
2041 ctttggggaa ttcattttat taaaaattgg tgtctggata cttccctgta catgcataaa
2101 tatgcatgca tgtacagaaa gactgtatgt gtgtgccttg cacacacacc catacctctc
2161 agaaaaagtg tttgggtatc ttaaaaactc gaaaaacaat gataaatttc tcagcttgtc
2221 cagacctgga acaaaatttc tggaataaga aatttgtatt aaagtccttt tttgcactaa
2281 cagttggctc ttgtagcctg caggctgagg aagtctcttc tctgtgcatc agcagagtta
2341 ctgaaagcct ctgattgaga aaaaacctcc gtctgcctaa atcacttttc tcgcagaagc
2401 catgcgactc ccacacgaca cgggcagctt cacaagccat ctctttcatt tctgcttgaa
2461 gcccccttgg ctgcagcaat cctgtctgcc ataggtttct tccttcctta cctactcaag
2521 ggctttttct aaggcatgca cacatatctc ctgttctctg agagtaccat ggtgttcctt
2581 aaaagaagaa aatttctaat tctgaactca atgttttgct tttactcccc ttctactgac
2641 aaatcatgat aagggcacaa aagctgtaca gatttttttt tttaaccact caatcccaaa
2701 tggaggccta caaagaacat cgtaataaca catggaggca aaccctgggt ttttaagagc
2761 aaattctgtc ccccctcact cccccaagtg acaagatact aatgaagaaa gttcttgacc
2821 atagtgtttg ttttaactaa actcattgga gtctagttcc agatttggta gggtcatcat
2881 ctctacattc cttaggattt ctctccctat caagctggcc cagatacaag taccaaacag
2941 tagtctctga agttccccca tttccttcag taccagtcta taagctactg tccgccactg
3001 attttcatct atcagggtgt cctaatcaga atcagccacc caagcaagcc tctctggccc
3061 acatatctat ctcttgcctt cccccatgaa cttcagcctg tccacacaaa agccacataa
3121 actcaagcaa gaaatatgtt cagccaaaac atgattatag tggcagctga ccaatccccc
3181 atcccaaatt gaccatttag atgtaccaac tcaccttaaa ttagcatgtt ccaatccagt
3241 cggcattgcc tgaatacagt agcatcatac ctatagttgg tcttagataa gaaatgaact
3301 acttgatata gcaaagtcct ttggcttcgt aaataaccct gaggttttgt acttactttc
3361 cccataggaa gacagaccat aggcaaactc tgttttggga tctcaactcc atcacctttg
3421 tttcaatatt ttttttctct cttgaacaaa actgagataa tttagaaaac aggtgcttaa
3481 ttgcaataaa attactatga agtatattaa aaatcacgac attgtaaaat ctcactttag
3541 atcatcaaag aaaaccattg ttactatctc ctttgagctt aggaaaatgt acaagagaac
3601 aaattaaaat tgaaaaattg atttcactta gaaaaacttc taggaacagg gtgaaccact
3661 gattttaatt tgcctaatta tcttatgaca agtatcaaat taagatgaca cttaaagatc
3721 cttagcatta acttaatgat ggagaagagt gctcaacaga cagttcccag taaggtaatg
3781 agatgccatt ttcagagaca ttctaagaag atattttgat tcattaaaac attaaataaa
3841 aagccctcct cagattggaa cccccaaatc gatggagcca cattaataat acttttcatg
3901 cctcactttg acatgacagc attcgatttt tttaaagatc tttaatactt tccatgagta
3961 ctaaagattg taatgagtta ccttatcctt agaagtagaa tgtttgcttt cttcttcttg
4021 gaaatagtcc tccaaaaagt ccacttgtac cagtgacaag aagtcacttg tatagtgacc
4081 aagtacacat aaaatactga ttataaaaat attgtaataa accacttcct catttgatac
4141 aggtattaag ctgaatcctc actaattcac ttttgaaaag ttcttaatga acagttttat
4201 tcttattcca tgaacagctc ttttaataca atttccttgg tatccaactt aaatcccaga
4261 attttgttcc accatggtta gttatttgcc atatgaatgt attcttgttt ctaccaatat
4321 tctaggcatg agaatacctt aatactgagt tggagatttt gtatctgttt ctcctccttt
4381 acgcccattt tcctacccac agcagcaaat gacaacgtgt ctgtccaggt ctgtccccct
4441 gctcatccct ggatgccact cacatttttt tcttcttgtt accttgacca cgctgtacag
4501 taacatccaa gagcccattc tacagtgggt ggttttggtc tttttataac tttttctcaa
4561 agtcactgat gtttgttcct gttaaatgta tagcattgta atgagagcac atcaaatcct
4621 gagtgtcagt ttgttgtccc tattgtagat gaaatagtga tgtagcaaaa acctagtaaa
4681 ttctgaatgc ttttccacgt agacttatct ggaatgtgaa cacaactctt tggttaatag
4741 taaatgctta actgtagtcc tgagtaggtg catttctgtc tgtctcaata aattttactt
4801 tgtctgcaaa aaaaaaaaaa aaa
//