LOCUS BC029820 1893 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens glycerol kinase 2, mRNA (cDNA clone MGC:35147
IMAGE:5169374), complete cds.
ACCESSION BC029820
VERSION BC029820.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1893)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1893)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-MAY-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 51 Row: c Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41393574.
FEATURES Location/Qualifiers
source 1..1893
/db_xref="H-InvDB:HIT000040888"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:35147 IMAGE:5169374"
/tissue_type="Brain, adult medulla"
/clone_lib="NIH_MGC_119"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1893
/gene="GK2"
/gene_synonym="GKTA"
/db_xref="GeneID:2712"
/db_xref="HGNC:HGNC:4291"
/db_xref="MIM:600148"
CDS 11..1672
/gene="GK2"
/gene_synonym="GKTA"
/codon_start=1
/product="glycerol kinase 2"
/protein_id="AAH29820.1"
/db_xref="GeneID:2712"
/db_xref="HGNC:HGNC:4291"
/db_xref="MIM:600148"
/translation="MAAPKTAAVGPLVGAVVQGTNSTRFLVFNSKTAELLSHHKVELT
QEFPKEGWVEQDPKEILQSVYECIARTCEKLDELNIDISNIKAVGVSNQRETTVIWDK
LTGEPLYNAVVWLDLRTQTTVEDLSKKIPGNSNFVKSKTGLPLSTYFSAVKLRWMLDN
VRNVQKAVEEGRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNIHSLEWDKE
LCDFFEIPMDLLPNVFSSSEIYGLIKTGALEGVPISGCLGDQCAALVGQMCFQEGQAK
NTYGTGCFLLCNTGRKCVFSEHGLLTTVAYKLGREKPAYYALEGSVAIAGAVIRWLRD
NLGIIETSGDIERLAKEVGTSYGCYFVPAFSGLYAPYWEPSARGILCGLTQFTNKCHI
AFAALEAVCFQTREILEAMNRDCGIPLRHLQVDGGMTNNKVLMQLQADILHIPVIKPF
MPETTALGAAMAAGAAEGVSVWSLEPQALSVLRMERFEPQIQATESEIRYATWKKAVM
KSMGWVTSQSPEGGDPSIFSSLPLGFFIVSSMVMLIGARYISGVP"
BASE COUNT 585 a 357 c 439 g 512 t
ORIGIN
1 tactggtgtc atggcagccc cgaagacagc agctgtgggg ccgttggtgg gagcggtggt
61 ccagggcacc aactccactc gctttctggt tttcaattca aaaacagcgg aactacttag
121 tcatcacaaa gtggaattaa cacaagagtt cccaaaagaa ggatgggtgg aacaagaccc
181 taaagaaatt cttcagtctg tctacgagtg tatagcgaga acgtgtgaga aacttgacga
241 actgaatatt gatatatcca acataaaagc tgttggtgtc agcaatcaga gggaaaccac
301 tgtaatctgg gacaagttaa caggagagcc tctctacaat gctgtggtgt ggcttgatct
361 aagaacccag actactgttg aggatcttag taaaaaaatt ccaggaaata gtaacttcgt
421 caagtctaag acaggccttc cactcagcac ttacttcagt gcagtaaaac ttcgttggat
481 gcttgacaat gtgagaaacg tccaaaaggc tgttgaagaa ggtagagctc tttttggtac
541 cattgattca tggcttatct ggagtttgac aggaggagtt aatggaggcg tgcattgtac
601 agatgtaaca aatgcaagta ggacaatgct ttttaatatc cattctttgg aatgggataa
661 agagctctgt gacttttttg aaattccaat ggaccttctt ccaaatgtct tcagttcttc
721 tgagatctat ggcctaatta aaactggagc cctggaaggt gtgccaatat ctgggtgttt
781 gggggaccaa tgtgctgcat tagtaggaca aatgtgcttc caggagggac aagccaaaaa
841 cacctatgga acaggttgct tcttactgtg taatacgggt cgtaaatgtg tgttttctga
901 acatggcctt ttgaccacag tagcttacaa actaggcaga gagaagccag catattatgc
961 actggaaggt tctgttgcta tagcaggtgc tgttattcgt tggctaagag acaatcttgg
1021 aattatagag acctcaggag acattgaaag acttgctaaa gaagtaggaa cttcttatgg
1081 ctgttacttt gtcccagcct tttcagggtt atatgcacct tattgggagc ccagtgcaag
1141 agggatactc tgtggcctca ctcagtttac caataaatgt catattgctt ttgctgcatt
1201 agaagctgtt tgtttccaaa cccgagagat tttggaagcc atgaaccgtg actgtggaat
1261 tccacttcgt catttgcagg tagatggagg aatgaccaac aacaaagttc ttatgcagct
1321 acaagcagat attcttcata ttccagtaat aaaacccttt atgcctgaaa caactgcact
1381 aggagctgcc atggcagcag gggctgcaga gggagtaagc gtttggagcc ttgaacccca
1441 ggctttgtca gttctcagga tggaacgatt tgaaccacag atccaggcca cagaaagtga
1501 aattcgttat gccacatgga agaaagccgt aatgaagtca atgggttggg ttaccagtca
1561 gtctcctgaa ggtggtgatc cttctatctt ctctagtctg cctttgggat tttttatagt
1621 gagtagcatg gtaatgctaa ttggagcaag atatatctcg ggtgtgccat aataatacca
1681 gctatggagt cccaagatgt aaacatttta cataatgaaa gcatatagca gttctgcctc
1741 ttaatataat gacactatta tagactctga ttttgtttat aaaccacttg ctacatgacc
1801 catgaaaacc ctagacttgt gacctgaaat aaagaaaata cgttagaaga gcaaaaaaaa
1861 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
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