LOCUS       BC029735                1635 bp    mRNA    linear   SYN 02-SEP-2016
DEFINITION  Synthetic construct Homo sapiens LIM and calponin homology domains
            1, mRNA (cDNA clone IMAGE:3857528), partial cds.
ACCESSION   BC029735
VERSION     BC029735.1
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2002) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: DCTD/DTP
            cDNA Library Preparation: Life Technologies, Inc.
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: Baylor College of Medicine Human Genome
            Sequencing Center
            Center code: BCM-HGSC
            Web site: http://www.hgsc.bcm.tmc.edu/cdna/
            Contact: amg@bcm.tmc.edu
            Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
            Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
            A.N., Gibbs, R.A.
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRAK Plate: 21 Row: b Column: 12.
FEATURES             Location/Qualifiers
     source          1..1635
                     /db_xref="H-InvDB:HIT000091891"
                     /organism="synthetic construct"
                     /mol_type="mRNA"
                     /isolation_source="Homo sapiens; Ovary, adenocarcinoma"
                     /db_xref="taxon:32630"
                     /clone="IMAGE:3857528"
                     /clone_lib="NIH_MGC_66"
                     /lab_host="DH10B"
                     /note="Vector: pCMV-SPORT6"
     gene            <1..1635
                     /gene="LIMCH1"
                     /gene_synonym="LIMCH1A"
                     /gene_synonym="LMO7B"
                     /db_xref="GeneID:22998"
                     /db_xref="HGNC:HGNC:29191"
     CDS             <1..982
                     /gene="LIMCH1"
                     /gene_synonym="LIMCH1A"
                     /gene_synonym="LMO7B"
                     /codon_start=2
                     /transl_table=11
                     /product="LIMCH1 protein"
                     /protein_id="AAH29735.1"
                     /db_xref="GeneID:22998"
                     /db_xref="HGNC:HGNC:29191"
                     /translation="DKMPEANQLHLPNLNSQVDSPSSEKSPVMTPFKFWAWDPEEERR
                     RQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFT
                     VSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESD
                     RLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNP
                     THSSEDVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNL
                     YFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL"
BASE COUNT          501 a          322 c          383 g          429 t
ORIGIN      
        1 ggacaaaatg cctgaagcca accaactaca tttgccaaat ctcaattctc aagtggattc
       61 tccaagcagt gagaagtcac ctgttatgac accttttaag ttctgggcat gggacccaga
      121 agaggagcgc aggcgacagg aaaaatggca acaggaacag gaacgtttgc tccaggagag
      181 ataccagaag gagcaggaca agctgaaaga agagtgggaa aaggcccaaa aggaggtgga
      241 agaggaagaa cgcagatact atgaggagga gcgtaagata attgaagaca ctgtggttcc
      301 atttactgtt tcttcaagtt ccgctgacca gctgtctacc tcttcctcca tgactgaagg
      361 cagtgggaca atgaataaga tagacctggg aaactgtcaa gatgaaaaac aagacagaag
      421 atggaagaaa tcattccagg gagatgacag tgacttattg ctgaagacta gggaaagtga
      481 tcgactggag gagaagggca gcctaactga aggggccttg gctcattctg ggaaccctgt
      541 atcaaaagga gtccatgaag accatcagct ggataccgag gctggggccc cacactgtgg
      601 aacaaaccca cagcttgctc aggatccatc ccagaatcag cagacatcaa atccaacgca
      661 cagttcagaa gatgtgaagc caaaaaccct cccgctggat aaaagcatta accatcagat
      721 cgagtctccc agtgaaaggc ggaagtctat aagtggaaag aagctgtgct cttcctgtgg
      781 gcttcctttg ggtaaaggag ctgcaatgat catcgagacc ctcaatctct attttcacat
      841 ccagtgtttc aggtgtggaa tttgtaaagg ccagcttgga gatgcagtga gtgggacgga
      901 tgttaggatt cgaaatggtc tcctgaactg taatgattgc tacatgcgat ccagaagtgc
      961 cgggcagcct acaacattgt gacacggctt tcaagcttcc ggatcactca ccatttcttt
     1021 actgagagtg tcccctggca actgcttaac aaaatcccaa gctcaggggc ttctcagcat
     1081 ttacctaatt tctgaaaggc tcttctgaaa ggtggtatct gttctttcgt agcacagtgt
     1141 ttatgttttt cctgtttatt gttttggttt tttttttttt ttgcatttgc acagtataca
     1201 caaaagaata tggggttgta atgatcctga atagctcaaa aaaggtttta gcatggtcaa
     1261 acaggcttat ggtttaaaat gtgttattct cttctttggg aattagctaa atgatgcaat
     1321 aaacctgttt tgttttagaa tgtctaggaa ttaaacactt tatgtttaca gaattgagct
     1381 gcagaaagtg caagacatgc caatttgaga cacacggtct tctaagactg aaggataaat
     1441 ttaatgcatt tcagaaacta aacatcacag caagctctat ctctgagcta taatttgttt
     1501 ttaatgcaaa gacactagtt tgataatata tactgtaatc ctgaaacatt tgtgttactt
     1561 acctttggag gtagaaatta taccaataaa ttattgcacc gttagtatta aaagttctag
     1621 atcgcgagcg gccgc
//