LOCUS BC028100 3559 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens kelch-like 25 (Drosophila), mRNA (cDNA clone MGC:39894
IMAGE:5242783), complete cds.
ACCESSION BC028100
VERSION BC028100.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3559)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3559)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 62 Row: m Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21362104
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..3559
/db_xref="H-InvDB:HIT000040440"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:39894 IMAGE:5242783"
/tissue_type="Brain, fetal, whole pooled"
/clone_lib="NIH_MGC_121"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3559
/gene="KLHL25"
/gene_synonym="ENC-2"
/gene_synonym="ENC2"
/db_xref="GeneID:64410"
/db_xref="HGNC:HGNC:25732"
CDS 123..1892
/gene="KLHL25"
/gene_synonym="ENC-2"
/gene_synonym="ENC2"
/codon_start=1
/product="KLHL25 protein"
/protein_id="AAH28100.1"
/db_xref="GeneID:64410"
/db_xref="HGNC:HGNC:25732"
/translation="MSVSVHETRKSRSSTGSMNVTLFHKASHPDCVLAHLNTLRKHCM
FTDVTLWAGDRAFPCHRAVLAASSRYFEAMFSHGLRESRDDTVNFQDNLHPEVLELLL
DFAYSSRIAINEENAESLLEAGDMLQFHDVRDAAAEFLEKNLFPSNCLGMMLLSDAHQ
CRRLYEFSWRMCLVHFETVRQSEDFNSLSKDTLLDLISSDELETEDERVVFEAILQWV
KHDLEPRKVHLPELLRSVRLALLPSDCLQEAVSSEALLMADERTKLIMDEALRCKTRI
LQNDGVVTSPCARPRKAGHTLLILGGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFS
ASAIGCKVYVTGGRGSENGVSKDVWVYDTVHEEWSKAAPMLIARFGHGSAELENCLYV
VGGHTSLAGVFPASPSVSLKQVEKYDPGANKWMMVAPLRDGVSNAAVVSAKLKLFVFG
GTSIHRDMVSKVQCYDPSENRWTIKAECPQPWRYTAAAVLGSQIFIMGGDTEFTAASA
YRFDCETNQWTRIGDMTAKRMSCHALASGNKLYVVGGYFGTQRCKTLDCYDPTSDTWN
CITTVPYSLIPTAFVSTWKHLPA"
BASE COUNT 723 a 1075 c 1081 g 680 t
ORIGIN
1 ttttgcctgt ggagagccgg ccgacgggag ccgcggcgga gcctgttgag ctcgcgcggg
61 ctgccgggag tggtctctga ggcggcggcg gcggcgggga tcgtctccgg cactggcgca
121 ccatgtcggt cagtgtccat gagacccgca agtcgcggag cagcacgggg tccatgaacg
181 tcaccctctt ccacaaggcc tcccacccgg actgtgtgct ggcccacctc aacacgcttc
241 gcaagcactg catgttcacc gacgtcacac tctgggcggg cgaccgtgcc ttcccctgtc
301 accgtgccgt gctggccgcc tctagccgct attttgaggc catgttcagc catggccttc
361 gggagagccg ggatgacact gtcaacttcc aggacaacct gcacccggag gtgctggagc
421 tgctgctgga ctttgcctac tcctcacgca tcgccatcaa cgaggagaac gctgagtcac
481 tgctggaggc aggcgacatg ctgcagttcc acgatgtgcg ggatgctgcc gccgagttcc
541 tggagaagaa ccttttcccc tccaactgcc tgggcatgat gctgctctcg gacgcccacc
601 agtgccgccg gctgtatgag ttctcctggc gcatgtgcct ggtgcacttt gagacggtga
661 ggcagagcga ggacttcaac agcctgtcca aggacacact gctggacctc atctcgagtg
721 atgagctgga gaccgaggac gagcgggtgg tcttcgaggc catcctccag tgggtgaagc
781 acgacctgga gccacggaag gtccacttgc ccgagctcct ccgcagcgtg cgtctggcct
841 tgctgccgtc cgactgcctg caggaggccg tctccagcga ggccctcctc atggcagacg
901 agcgcaccaa gcttatcatg gatgaggccc tgcgctgcaa gaccaggatc ctgcagaatg
961 atggcgtggt caccagcccc tgtgcccggc cacgcaaggc gggccacacg ctactcatcc
1021 tggggggcca gaccttcatg tgtgacaaga tctaccaggt ggaccacaag gccaaggaga
1081 tcatccccaa ggccgacctg cccagccccc ggaaggagtt cagcgcctca gcgatcggct
1141 gcaaggtcta tgtgacgggg ggcaggggct ccgagaacgg ggtctccaag gatgtctggg
1201 tgtacgacac cgtacatgag gaatggtcca aggcggcgcc catgctgatt gcccgctttg
1261 gccatggctc agctgagctg gagaactgcc tctatgtggt ggggggacac acatccctgg
1321 caggggtctt cccggcctcg ccttctgtct ccctgaaaca agtggagaaa tacgaccctg
1381 gggccaacaa gtggatgatg gtggccccct tgcgggatgg cgtcagcaat gccgcagtgg
1441 tgagtgccaa gctgaagctc tttgttttcg gaggaaccag catccaccgg gacatggtgt
1501 ccaaggtcca gtgctatgac ccctcggaga acaggtggac gatcaaggcc gagtgccccc
1561 agccttggcg gtacacagcc gctgccgtcc tgggcagcca gatcttcatc atgggaggtg
1621 acacggaatt cacagccgcc tcggcctacc gctttgactg tgagaccaac cagtggacgc
1681 ggattgggga catgactgcc aagcgcatgt cctgccatgc cctggcttcc ggcaacaagc
1741 tctatgtggt cgggggctac tttgggaccc agaggtgtaa gactctggac tgctatgacc
1801 ccacttcaga tacatggaac tgcatcacca cagtgcccta ctcacttatc cccacggcct
1861 ttgtcagcac ctggaagcac ctgcccgcgt gaggagcacc tgctgagccc agccagaccg
1921 cggccttcag tgtcacagcg tggccttgct tgtctgccac agcgggagct aagccggccc
1981 tgggccagca ctccgagagg tggaaggggc cctgccagct ctggggagca gcagccttgg
2041 gctgttctga gctttaggca agagaagaga agcatctctt gcatccgtgc ccctgggggc
2101 ctcttcagct ttgcagtggt ttgtgggaag acatacctcc cagaggggca tggactgcca
2161 ccaggactga ccctggcgtc ggggagaagg acacttgcag agccttgaga tcacctgttt
2221 ggcaggtcct ggactggggc tctactgcca tctatagatg gtgtcctggg cctgggaaac
2281 taggttccca ggggttgaga ccagaaaggt gaccaagaca gattttttaa ggtgcagaaa
2341 ctgcaggggg gcctcagtga catccatgag gccttattag caaaggacat ccagacctcc
2401 aaggtttgtg ggccccttcc acaaagctgt aagtcccagc ccacctactc agggccttgc
2461 tcagtgctgt ggcccggtgg ggacacagtt gctcgtggcc actcagtgga gctgggcctg
2521 cagcagactc aaggctccga gtgccctggg ggtcacccct cccctcccct cctcagagcc
2581 caccctgaga ggcagcagtg acccccatgg cacacacctg ccaacagcac tgggggcttc
2641 tccccaggag accacgctgt cctccaagac caggagcagc tgtgagctgg agacagcaga
2701 gggaccccag ggtgtcccct gcagatccca ccagggccgc atccatctca gtgtggagga
2761 cagtgacggg accctcacca tcctcttgcg ttttggcccc catttgctcc ctgagctcca
2821 agataagaat ggccccgaga gaactgctga acatttgttc attgctgtca cctcctgagt
2881 cactggggtc cctcaccagc acctccctga cacctgggct atggagaggt tggcgcctgt
2941 cagtgaccat cctaatgcct ctcgctcact cccaagccac catttgagag ggaggggtgt
3001 tggtgccctg acagggactg ggcagggtgt ccaaacttgg ggcttcccag gcacctgcag
3061 tgtgaacact gcttggctgg ctcaagatta gggccgcgga gggggctgtg cacataccag
3121 ttacttaagc agccacgagt gtcccccatg ccttggtgcg ggtcctggag gcctcttggg
3181 ggtgggacct ttgggcaggg tttgcccact gacacgcccg ccatggggca ctggctgcat
3241 ggggctcctt ggaccctgta gacccagcag accccgcctg tgtcaggcca gctgcccgaa
3301 ggcacttggc tgtggtccca ggggacggct ctgctggcac actcaggagc ctggccgcgg
3361 ggactgcagg gagggtgcct ggacccgtgg ggttgcttca ttgagataaa gcacacttat
3421 cacatagcac aaaggacgtg ccatggtgct ttccccaaaa gttgtgttgc ttttatcagt
3481 tttctaactt aataaaaaga gttgagaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3541 aaaaaaaaaa aaaaaaaaa
//