LOCUS BC027962 2656 bp mRNA linear HUM 03-FEB-2004
DEFINITION Homo sapiens SH2 domain containing 3C, mRNA (cDNA clone MGC:34461
IMAGE:5223012), complete cds.
ACCESSION BC027962
VERSION BC027962.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2656)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2656)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (08-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 49 Row: l Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4885526.
FEATURES Location/Qualifiers
source 1..2656
/db_xref="H-InvDB:HIT000040379"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:34461 IMAGE:5223012"
/tissue_type="Pancreas, Spleen, adult pooled"
/clone_lib="NIH_MGC_120"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2656
/gene="SH2D3C"
/gene_synonym="CHAT"
/gene_synonym="NSP3"
/db_xref="GeneID:10044"
/db_xref="MIM:604722"
CDS 12..2123
/gene="SH2D3C"
/gene_synonym="CHAT"
/gene_synonym="NSP3"
/codon_start=1
/product="SH2D3C protein"
/protein_id="AAH27962.1"
/db_xref="GeneID:10044"
/db_xref="MIM:604722"
/translation="MTAVGRRCPALGSRGAAGEPEAGSDYVKFSKEKYILDSSPEKLH
KELEEELKLSSTDLRSHAWYHGRIPREVSETLVQRNGDFLIRDSLTSLGDYVLTCRWR
NQALHFKINKVVVKAGESYTHIQYLFEQESFDHVPALVRYHVGSRKAVSEQSGAIIYC
PVNRTFPLRYLEASYGLGQGSSKPASPVSPSGPKGSHMKRRSVTMTDGLTADKVTRSD
GCPTSTSLPRPRDSIRSCALSMDQIPDLHSPMSPISESPSSPAYSTVTRVHAAPAAPS
ATALPASPVARRSSEPQLCPGSAPKTHGESDKGPHTSPSHTLGKASPSPSLSSYSDPD
SGHYCQLQPPVRGSREWAATETSSQQARSYGERLKELSENGAPEGDWGKTFTVPIVEV
TSSFNPATFQSLLIPRDNRPLEVGLLRKVKELLAEVDARTLARHVTKVDCLVARILGV
TKEMQTLMGVRWGMELLTLPHGRQLRLDLLERFHTMSIMLAVDILGCTGSAEERAALL
HKTIQLAAELRGTMGNMFSFAAVMGALDMAQISRLEQTWVTLRQRHTEGAILYEKKLK
PFLKSLNEGKEGPPLSNTTFPHVLPLITLLECDSAPPEGPEPWGSTEHGVEVVLAHLE
AARTVAHHGGLYHTNAEVKLQGFQARPELLEVFSTEFQMRLLWGSQGASSSQARRYEK
FDKVLTALSHKLEPAVRSSEL"
misc_feature 198..497
/gene="SH2D3C"
/gene_synonym="CHAT"
/gene_synonym="NSP3"
/note="SH2; Region: Src homology 2 domains"
/db_xref="CDD:cd00173"
misc_feature 1293..1802
/gene="SH2D3C"
/gene_synonym="CHAT"
/gene_synonym="NSP3"
/note="RasGEF; Region: Guanine nucleotide exchange factor
for Ras-like small GTPases. Small GTP-binding proteins of
the Ras superfamily function as molecular switches in
fundamental events such as signal transduction,
cytoskeleton dynamics and intracellular trafficking.
Guanine-nucleotide-exchange factors (GEFs) positively
regulate these GTP-binding proteins in response to a
variety of signals. GEFs catalyze the dissociation of GDP
from the inactive GTP-binding proteins. GTP can then bind
and induce structural changes that allow interaction with
effectors"
/db_xref="CDD:cd00155"
BASE COUNT 622 a 850 c 724 g 460 t
ORIGIN
1 gcgggggaag catgactgct gtgggccgaa ggtgccccgc gctggggtcc cgaggggctg
61 ctggagagcc agaggctggc agcgactatg tgaagttctc caaggagaag tacatcctgg
121 actcatcgcc agagaaactc cacaaggaat tggaggagga gctcaaactc agcagcacgg
181 atctccgcag ccatgcctgg taccatggcc gcatcccccg agaggtctcg gagaccttgg
241 tacaacgcaa cggcgacttc ctcatccggg actcactcac cagcctgggc gactatgtgc
301 tcacgtgccg ctggcgcaac caggccttgc acttcaagat caacaaggtg gtggtgaagg
361 caggcgagag ctacacacac atccagtacc tgtttgagca ggagagcttt gaccacgtgc
421 ccgccctcgt gcgctatcat gtgggcagcc gcaaggctgt gtcagagcag agtggtgcca
481 tcatctactg cccggtgaac cgcaccttcc cactgcgcta cctcgaggcc agctatggcc
541 tgggacaggg gagtagcaag cctgctagcc ccgtcagccc ctcaggcccc aagggcagcc
601 acatgaagcg gcgcagcgtc accatgaccg atgggctcac tgctgacaag gtcacccgca
661 gcgatggctg ccccaccagt acgtcgctgc cccgccctcg ggactccatc cgcagctgtg
721 ccctcagcat ggaccagatc ccagacctgc actcacccat gtcgcccatc tccgagagcc
781 ctagctcccc tgcctacagc actgtaaccc gtgtccatgc cgcccctgca gccccttctg
841 ccacagcatt gcctgcctcc cctgtcgccc gccgttccag tgagccccag ctgtgtcccg
901 gaagtgcccc aaagacccat ggggagtcag acaagggccc ccacaccagc ccctcccaca
961 cccttggcaa ggcctccccg tcaccatcac tcagcagcta cagtgacccg gactctggcc
1021 actactgcca gctccagcct cccgtgcgtg gcagccgaga gtgggcagcg actgagacct
1081 ccagccagca ggccaggagc tatggggaga ggctaaagga actgtcagaa aatggggccc
1141 ctgaagggga ctggggcaag accttcacag tccccatcgt ggaagtcact tcttccttca
1201 acccggccac cttccagtca ctactgatcc ccagggataa ccggccactg gaggtgggcc
1261 ttctgcgcaa ggtcaaggag ctgctggcag aagtggatgc ccggacgctg gcccggcatg
1321 tcaccaaggt ggactgcctg gttgctagga tactgggcgt taccaaggag atgcagaccc
1381 taatgggagt ccgctggggc atggaactgc tcaccctccc ccatggccgg cagctacgcc
1441 tagacctgct ggaaaggttc cacaccatgt ccatcatgct ggccgtggac atcctgggct
1501 gcaccggctc tgcggaggag cgggcagcgc tgctgcacaa gaccattcag ctggcggccg
1561 agctgcgggg gactatgggc aacatgttca gcttcgcggc ggtcatgggt gccctggaca
1621 tggctcagat ttctcggctg gagcagacat gggtgaccct gcggcagcga cacacagagg
1681 gtgccatcct gtacgagaag aagctcaagc cttttctcaa gagcctcaac gagggcaaag
1741 aaggcccgcc gctgagcaac accacgtttc ctcatgtgct gcccctcatc accctgctgg
1801 agtgtgactc ggccccacca gagggccctg agccctgggg cagcacggag cacggcgtgg
1861 aggtggtgct ggctcacctg gaggccgccc gcacagtggc acaccacgga ggcctgtacc
1921 acaccaatgc tgaagtcaag ctgcaggggt tccaggcccg gccggagctc ctggaggtgt
1981 tcagcacgga gttccagatg cgccttctct ggggcagtca gggtgccagc agcagccagg
2041 cccggcgcta tgagaagttc gacaaggtcc tcactgccct gtcccacaag ctggaacctg
2101 ctgtccgctc cagcgagctg tgaccccagg gacatttccc ctctgcagct gcggacagcg
2161 tcaggggcag aggggcacac aactttcccc agagcacccc aaggacactg tgatcaaccc
2221 gagaatgttc tgggttcaac tcaagcatct cccttgcacc tccagggtcc tgcgtggact
2281 ctgggttcca tcccacctgc tacatgctca ccaggtctcc attgaggaag aacaggaacg
2341 ccggttcccc caccagcttt tgctgctccc cttcctgctg gggttccctg ttttcgagcc
2401 atgggaggca ggctgctcac gcctcctcac tctctgtctg tccctcacca acaccaaggc
2461 ctccatctca ctgtaaataa gtctctgttc tgtaaataga tgtacagaag ccatgttatt
2521 tctttcatat aataaacttt tatgactctt taaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2581 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2641 aaaaaaaaaa aaaaaa
//