LOCUS BC027849 4670 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens RAS guanyl releasing protein 3 (calcium and
DAG-regulated), mRNA (cDNA clone MGC:34340 IMAGE:5203041), complete
cds.
ACCESSION BC027849
VERSION BC027849.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4670)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4670)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 49 Row: e Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 24762238.
FEATURES Location/Qualifiers
source 1..4670
/db_xref="H-InvDB:HIT000050925"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:34340 IMAGE:5203041"
/tissue_type="Lung, Spleen, fetal, pooled"
/clone_lib="NIH_MGC_122"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..4670
/gene="RASGRP3"
/gene_synonym="GRP3"
/gene_synonym="KIAA0846"
/db_xref="GeneID:25780"
/db_xref="HGNC:HGNC:14545"
/db_xref="MIM:609531"
CDS 628..2700
/gene="RASGRP3"
/gene_synonym="GRP3"
/gene_synonym="KIAA0846"
/codon_start=1
/product="RAS guanyl releasing protein 3 (calcium and
DAG-regulated)"
/protein_id="AAH27849.1"
/db_xref="GeneID:25780"
/db_xref="HGNC:HGNC:14545"
/db_xref="MIM:609531"
/translation="MGSSGLGKAATLDELLCTCIEMFDDNGELDNSYLPRIVLLMHRW
YLSSTELAEKLLCMYRNATGESCNEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEF
REVASQLGYEKHVSLIDISSIPSYDWMRRVTQRKKVSKKGKACLLFDHLEPIELAEHL
TFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVLSKPTPQQR
AEVITKFINVAKKLLQLKNFNTLMAVVGGLSHSSISRLKETHSHLSSEVTKNWNEMTE
LVSSNGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWTEENKVNIVKMHQLS
VTLSELVSLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSP
TTPNKPVVPLEWALGVMPKPDPTVINKHIRKLVESVFRNYDHDHDGYISQEDFESIAA
NFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMTYLKPTFC
EHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRFARAPSLSSGHGSLPGSPSL
PPAQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAG
WGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPRVHAGVDVVDRGTEFELDQDEGEE
TRQDGEDG"
BASE COUNT 1475 a 941 c 958 g 1296 t
ORIGIN
1 ttgagctcaa agcttagcag cttcaatgtt gttgttaggc ataaacagct tgcaaatttc
61 aatttttttt ctctcctcac ttttgaacta gatttgggca atggaggtga tgacagcatt
121 ttagaagaca gaacaggaat tctaggtgtt taggcacgtt gaagagaaac ttgctagagg
181 tgatgaagtg gaaagaaaaa gacaattcac tatagggatc aacattattg cagttctgca
241 aaggatattt tcttttaaaa catccatatc tcttctttgc catggaaact acacccaatc
301 tacctgaacc tgcggcaccc ctctgatgac ccatttggat tctctgattt cactgccaag
361 attctcagtt ctccaaaata cttatcattc aggttgaata aaccagttct acagactgct
421 tcttccatgc atttcccaat gatgctaact gcccttaaaa ttctttcaga tttggaactg
481 tatctgtatg gaaacaacag agttttcttg aagtacaact aaggacaggt caccactagg
541 cactaacatc gctgacttgc atgattatgg agatggtcta tctgatgctg aaaatgtctc
601 tagttttttg acaacggcta aataaccatg ggatcaagtg gccttgggaa agcagcaaca
661 ttagatgaac tgctgtgcac ttgcattgag atgtttgatg acaatggaga gctggataat
721 agttatttgc caagaatagt tctactgatg caccgatggt atttatcttc cactgaattg
781 gcagaaaaac ttctctgcat gtatcgaaat gccactggag aaagctgcaa tgaatttcga
841 ttaaagatct gctacttcat gaggtactgg attctgaagt ttcctgcaga gtttaatttg
901 gatcttggtt tgattcgtat gactgaggaa tttcgggaag tagctagtca actaggatat
961 gaaaaacacg tcagcctcat cgacatatcc agcattcctt cctatgactg gatgagaaga
1021 gtcacacaga ggaaaaaagt atccaagaag ggaaaagcct gtctgctgtt tgaccatctg
1081 gagcccattg aattggctga gcacctcact tttctggagc ataaatcttt tagaaggatc
1141 tcattcactg attaccaaag ctatgtcatc catggctgcc tggagaataa tccaaccttg
1201 gaaagatcga ttgctttatt taatggaatc tctaagtggg tccagttgat ggttcttagc
1261 aaaccaaccc cccagcaaag ggcagaagtc atcacaaagt ttatcaatgt tgcaaagaag
1321 ctccttcagc tcaaaaattt taacaccctg atggcagtgg tgggaggcct cagtcatagt
1381 tccatttcac gcctcaaaga gacccattct catctttctt cagaagttac aaagaactgg
1441 aatgaaatga cagagttggt ctcctccaac ggcaattact gcaattaccg caaggccttt
1501 gccgactgcg atggcttcaa aatccccatc cttggagtac acttgaaaga cttgatagct
1561 gtccatgtca ttttcccaga ctggacagag gagaacaaag tgaacattgt gaaaatgcac
1621 cagctctccg ttaccctgag tgaactagtc tccctgcaga atgcctctca ccacttagaa
1681 cccaacatgg atttgatcaa cctgctcacg ctttccctgg acctctatca cactgaagat
1741 gatatttaca aactgtcact ggtgctggag cctagaaatt ctaaatcgca gcctacctcc
1801 cctacgacgc ccaacaagcc tgtggtaccc ctggagtggg cattaggggt gatgccaaag
1861 ccagacccca cggtcatcaa caagcacata aggaaattag tggagtctgt atttagaaac
1921 tatgatcacg accatgatgg gtacatttcc caagaggact ttgaaagtat agctgccaat
1981 tttcccttct tggattcctt ctgtgttctg gacaaagatc aggatggcct aattagtaaa
2041 gatgaaatga tggcttactt cctgagagct aaatcccaac tacactgtaa aatgggacca
2101 ggatttatcc ataattttca ggagatgacc tatctcaagc caaccttctg cgaacactgt
2161 gcgggatttc tctggggcat aatcaagcaa ggatacaaat gcaaagactg tggagccaat
2221 tgtcacaaac agtgcaaaga cctcctggtt ctggcctgca ggagatttgc ccgggcgccc
2281 tccttgagca gtggtcatgg gtcactgcct ggaagcccct cgctgccccc agcgcaggat
2341 gaggtgtttg agttccctgg agtcactgct ggacacaggg atttagacag cagagccatc
2401 acactggtta caggctcttc tcgcaagatc tctgtgaggc tacagagggc caccaccagc
2461 caggccaccc agactgaacc tgtctggtca gaggctggct ggggggactc ggggtcccac
2521 accttcccta aaatgaaatc caagttccat gacaaagcag caaaggacaa aggctttgcc
2581 aaatgggaaa atgagaagcc cagggtgcat gctggtgtgg atgttgtaga ccggggcacg
2641 gagtttgaac ttgaccagga tgaaggagaa gagaccagac aggatggtga ggatggctga
2701 cttcaggctg cggaaactga aggcaataat gttggctttt ggaaggggca agacgagaaa
2761 ctctgaagaa agctctgact ctcaggaagt tatctggaaa gatacctgga tgtttactgc
2821 cttgggacac tgtgggatct ccatgtttgg actatgggac agagaattga ccctaactaa
2881 ctatgaacta tttatttcct cctcccctac ccctagttaa gtgccacaaa gactgtgtta
2941 tgtagtcagt acttttttct catgtatctt tctctagacc atttatatac gggtgaaatg
3001 aaagcctttt tgcatgtact tgatactcag tctgtaaact cagacttcgc tttttttgtg
3061 agactatcct ttcaatattt ttataaactt ttgtgtgtgc tgtggcaggg aacagttaac
3121 aaggagttta tttagagggg tttttttcat tttttgtttt tcttgttttg ttttgttttg
3181 ttttgttctt cttggtgtgc tttttggatg ggaccagaac ttaacatttt cctgaggact
3241 aggaagctca tcagacactg gaatgcagtg attcttctaa tgatggtcaa ctgcaaggag
3301 aaactgttta catccttccc actacacagt tgctatgata tgtaacaagt cacacatgta
3361 tatatcacac aagtcttacc aattctgcgt cactaggaag ctatagcatg gtcgtgaaag
3421 ctctcctaat acttacttaa atagccatgg aagaaaatta tgcttgtccc ttctgactgg
3481 gttaagccat ctttcttaag attcctgaag tagaaatagc aattaagaaa ttaattcaag
3541 ttttgacggt taacatttag cagaaaaaca aaccattgaa taagctacta ggtgaaatgc
3601 aaaacaacca tcaatttttt taaactcaat aattttgtga aaaaaaaaac caccaccaaa
3661 cttttggttg taaaagagaa cccttgttcc cttcctaaga aactgccttc cacaattaaa
3721 gaatactcat agaaagaaaa taatcataaa tcatatttga ttaacatttc atttaagctt
3781 ttaaaatatc aattttttaa atagctccca tttacccaaa ataactagtt atgggtctat
3841 ttcttccatg attagaggag agagaacttg attcaagata ctgaaaaata gagctgggac
3901 tgagcctgtg aatgacagga atgatctatt accaggtgac tagtcggaaa ctacacagac
3961 gtgcactggt ctgcatttgg gggctaaagt gtatatattc catttaaaat ggaatttgtt
4021 tgtatttggg gcagtcttca gtaaagcctt tctcctttct tttctccatt gaggacagtt
4081 ctgctcagca aaattgttga gtacctgttc tgggcaggtc ccacgctata tgcacaaagt
4141 taagaaaaac ttggtcttag cccttgggag ctgacagccc atgggcatta aggcaaagta
4201 gttccagtga tttaaaatac ggttccaaat acgctaaaac caacttgtgc caaccagatt
4261 tacagattgg aaatactgca gatgatgtga agttatcagt tggaggagct gtgattaagc
4321 tggataataa gagaacgtgc catctgtaaa gcactcagaa ggcagccatc cctagatgtt
4381 ggtttcatgt atattacact atctactact atccataaat gcaataatat gcatgttaac
4441 aacattaaaa acagcaaaca gcaatctaag tacagaaaag ctttttgtgt gtttaaaaaa
4501 attgaagaaa attcaggaag aaacgtgtta ataaacattg tactgttctt aaaaaaaaaa
4561 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
4621 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//