LOCUS BC025925 1312 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, mRNA (cDNA
clone MGC:15497 IMAGE:2989348), complete cds.
ACCESSION BC025925
VERSION BC025925.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1312)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1312)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (18-MAR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Louis M. Staudt, M.D., Ph.D.
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 22 Row: e Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..1312
/db_xref="H-InvDB:HIT000040030"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15497 IMAGE:2989348"
/tissue_type="Lymph, Burkitt lymphoma"
/clone_lib="NIH_MGC_8"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1312
/gene="GAPDH"
/gene_synonym="G3PD"
/db_xref="GeneID:2597"
/db_xref="HGNC:HGNC:4141"
/db_xref="MIM:138400"
CDS 58..1065
/gene="GAPDH"
/gene_synonym="G3PD"
/codon_start=1
/product="glyceraldehyde-3-phosphate dehydrogenase"
/protein_id="AAH25925.1"
/db_xref="GeneID:2597"
/db_xref="HGNC:HGNC:4141"
/db_xref="MIM:138400"
/translation="MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMV
YMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVF
TTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPL
AKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVG
KVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGY
TEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASK
E"
BASE COUNT 344 a 381 c 326 g 261 t
ORIGIN
1 cgacagttag ccgcatcttc ttttgcgtcg ccagccgagc cacatcgctg agacaccatg
61 gggaaggtga aggtcggagt caacggattt ggtcgtattg ggcgcctggt caccagggct
121 gcttttaact ctggtaaagt ggatattgtt gccatcaatg accccttcat tgacctcaac
181 tacatggttt acatgttcca atatgattcc acccatggca aattccatgg caccgtcaag
241 gctgagaacg ggaagcttgt catcaatgga aatcccatca ccatcttcca ggagcgagat
301 ccctccaaaa tcaagtgggg cgatgctggc gctgagtacg tcgtggagtc cactggcgtc
361 ttcaccacca tggagaaggc tggggctcat ttgcaggggg gagccaaaag ggtcatcatc
421 tctgccccct ctgctgatgc ccccatgttc gtcatgggtg tgaaccatga gaagtatgac
481 aacagcctca agatcatcag caatgcctcc tgcaccacca actgcttagc acccctggcc
541 aaggtcatcc atgacaactt tggtatcgtg gaaggactca tgaccacagt ccatgccatc
601 actgccaccc agaagactgt ggatggcccc tccgggaaac tgtggcgtga tggccgcggg
661 gctctccaga acatcatccc tgcctctact ggcgctgcca aggctgtggg caaggtcatc
721 cctgagctga acgggaagct cactggcatg gccttccgtg tccccactgc caacgtgtca
781 gtggtggacc tgacctgccg tctagaaaaa cctgccaaat atgatgacat caagaaggtg
841 gtgaagcagg cgtcggaggg ccccctcaag ggcatcctgg gctacactga gcaccaggtg
901 gtctcctctg acttcaacag cgacacccac tcctccacct ttgacgctgg ggctggcatt
961 gccctcaacg accactttgt caagctcatt tcctggtatg acaacgaatt tggctacagc
1021 aacagggtgg tggacctcat ggcccacatg gcctccaagg agtaagaccc ctggaccacc
1081 agccccagca agagcacaag aggaagagag agaccctcac tgctggggag tccctgccac
1141 actcagtccc ccaccacact gaatctcccc tcctcacagt tgccatgtag accccttgaa
1201 gaggggaggg gcctagggag ccgcaccttg tcatgtacca tcaataaagt accctgtgct
1261 caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
//