LOCUS BC025737 2169 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pyruvate kinase, liver and RBC, mRNA (cDNA clone
MGC:34243 IMAGE:5221697), complete cds.
ACCESSION BC025737
VERSION BC025737.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2169)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2169)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (06-MAR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 49 Row: k Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 32967595.
FEATURES Location/Qualifiers
source 1..2169
/db_xref="H-InvDB:HIT000039996"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:34243 IMAGE:5221697"
/tissue_type="Pancreas, Spleen, adult pooled"
/clone_lib="NIH_MGC_120"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2169
/gene="PKLR"
/gene_synonym="PK1"
/gene_synonym="PKL"
/gene_synonym="RPK"
/db_xref="GeneID:5313"
/db_xref="HGNC:HGNC:9020"
/db_xref="MIM:609712"
CDS 26..1750
/gene="PKLR"
/gene_synonym="PK1"
/gene_synonym="PKL"
/gene_synonym="RPK"
/codon_start=1
/product="pyruvate kinase, liver and RBC"
/protein_id="AAH25737.1"
/db_xref="GeneID:5313"
/db_xref="HGNC:HGNC:9020"
/db_xref="MIM:609712"
/translation="MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASV
AQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVE
RLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGP
EIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIY
IDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGV
EHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIM
VARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVA
NAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRD
PTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHL
CRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTN
IMRVLSIS"
BASE COUNT 512 a 635 c 607 g 415 t
ORIGIN
1 gcagccccag gcccacactg aaagcatgtc gatccaggag aacatatcat ccctgcagct
61 tcggtcatgg gtctctaagt cccaaagaga cttagcaaag tccatcctga ttggggctcc
121 aggagggcca gcggggtatc tgcggcgggc cagtgtggcc caactgaccc aggagctggg
181 cactgccttc ttccagcagc agcagctgcc agctgctatg gcagacacct tcctggaaca
241 cctctgccta ctggacattg actccgagcc cgtggctgct cgcagtacca gcatcattgc
301 caccatcggg ccagcatctc gctccgtgga gcgcctcaag gagatgatca aggccgggat
361 gaacattgcg cgactcaact tctcccacgg ctcccacgag taccatgctg agtccatcgc
421 caacgtccgg gaggcggtgg agagctttgc aggttcccca ctcagctacc ggcccgtggc
481 catcgccctg gacaccaagg gaccggagat ccgcactggg atcctgcagg ggggtccaga
541 gtcggaagtg gagctggtga agggctccca ggtgctggtg actgtggacc ccgcgttccg
601 gacgcggggg aacgcgaaca ccgtgtgggt ggactacccc aatattgtcc gggtcgtgcc
661 ggtggggggc cgcatctaca ttgacgacgg gctcatctcc ctagtggtcc agaaaatcgg
721 cccagaggga ctggtgaccc aagtggagaa cggcggcgtc ctgggcagcc ggaagggcgt
781 gaacttgcca ggggcccagg tggacttgcc cgggctgtcc gagcaggacg tccgagacct
841 gcgcttcggg gtggagcatg gggtggacat cgtctttgcc tcctttgtgc ggaaagccag
901 cgacgtggct gccgtcaggg ctgctctggg tccggaagga cacggcatca agatcatcag
961 caaaattgag aaccacgaag gcgtgaagag gtttgatgaa atcctggagg tgagcgacgg
1021 catcatggtg gcacgggggg acctaggcat cgagatccca gcagagaagg ttttcctggc
1081 tcagaagatg atgattgggc gctgcaactt ggcgggcaag cctgttgtct gtgccacaca
1141 gatgctggag agcatgatta ccaagccccg gccaacgagg gcagagacaa gcgatgtcgc
1201 caatgctgtg ctggatgggg ctgactgcat catgctgtca ggggagactg ccaagggcaa
1261 cttccctgtg gaagcggtga agatgcagca tgcgattgcc cgggaggcag aggccgcagt
1321 gtaccaccgg cagctgtttg aggagctacg tcgggcagcg ccactaagcc gtgatcccac
1381 tgaggtcacc gccattggtg ctgtggaggc tgccttcaag tgctgtgctg ctgccatcat
1441 tgtgctgacc acaactggcc gctcagccca gcttctgtct cggtaccgac ctcgggcagc
1501 agtcattgct gtcacccgct ctgcccaggc tgcccgccag gtccacttat gccgaggagt
1561 cttccccttg ctttaccgtg aacctccaga agccatctgg gcagatgatg tagatcgccg
1621 ggtgcaattt ggcattgaaa gtggaaagct ccgtggcttc ctccgtgttg gagacctggt
1681 gattgtggtg acaggctggc gacctggctc cggctacacc aacatcatga gggtgctaag
1741 catatcctga gacgcccctc ccccctctgg cccagcctac ccttgtaccc catcccttcc
1801 tccccagtct acgttctcca gcccacaccc ctccaaagcc ccacctttaa gtcctctctt
1861 ctctattcct gaccctccct acctgaggcc tatctgagac tataactgtc atctagcccc
1921 ttcgaggttg ccccttcccc atctccattt cacacaggtc ctgaaagtct gtgtccaatt
1981 atgcactggc cacccaacag caccaattgt acattctctg catccaatct gctcagcagg
2041 ccctaagatg ccttgagtct ttaatcccaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2101 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2161 aaaaaaaaa
//