LOCUS BC023635 1287 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens lipoic acid synthetase, mRNA (cDNA clone MGC:23245
IMAGE:4841470), complete cds.
ACCESSION BC023635
VERSION BC023635.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1287)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1287)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 19, 2003 this sequence version replaced BC023635.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 33 Row: n Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 37577165.
FEATURES Location/Qualifiers
source 1..1287
/db_xref="H-InvDB:HIT000050856"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23245 IMAGE:4841470"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1287
/gene="LIAS"
/gene_synonym="HUSSY-01"
/gene_synonym="LAS"
/gene_synonym="LIP1"
/gene_synonym="MGC23245"
/db_xref="GeneID:11019"
/db_xref="HGNC:HGNC:16429"
/db_xref="MIM:607031"
CDS 40..1158
/gene="LIAS"
/gene_synonym="HUSSY-01"
/gene_synonym="LAS"
/gene_synonym="LIP1"
/gene_synonym="MGC23245"
/codon_start=1
/product="lipoic acid synthetase"
/protein_id="AAH23635.1"
/db_xref="GeneID:11019"
/db_xref="HGNC:HGNC:16429"
/db_xref="MIM:607031"
/translation="MSLRCGDAARTLGPRVFGRYFCSPVRPLSSLPDKKKELLQNGPD
LQDFVSGDLADRSTWDEYKGNLKRQKGERLRLPPWLKTEIPMGKNYNKLKNTLRNLNL
HTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDASEP
YNTAKAIAEWGLDYVVLTSVDRDDMPDGGAEHIAKTVSYLKERNPKILVECLTPDFRG
DLKAIEKVALSGLDVYAHNVETVPELQSKVRDPRVNFDQSLRVLKHAKKVQPDVISKT
SIMLGLGENDEQVYATMKALREADVDCLTLGQYMQPTRRHLKVEEYITPEKFKYWEKV
GNELGFHYTASGPLVRSSYKAGEFFLKNLVAKRKTKDL"
BASE COUNT 420 a 248 c 295 g 324 t
ORIGIN
1 atccctcaac ccctgcactg cgctagtcct aaagaggaaa tgtctctacg ctgcggggat
61 gcagcccgca ccctggggcc ccgggtattt gggagatatt tttgcagccc agtcagaccg
121 ttaagctcct tgccagataa aaaaaaggaa ctcctacaga atggaccaga ccttcaagat
181 tttgtatctg gtgatcttgc agacaggagc acctgggatg aatataaagg aaacctaaaa
241 cgccagaaag gagaaaggtt aagactacct ccatggctaa agacagagat tcccatgggg
301 aaaaattaca ataaactgaa aaatactttg cggaatttaa atctccatac agtatgtgag
361 gaagctcgat gtcccaatat tggagagtgt tggggaggtg gagaatatgc caccgccaca
421 gccacgatca tgttgatggg tgacacatgt acaagaggtt gcagattttg ttctgttaag
481 actgcaagaa atcctcctcc actggatgcc agtgagccct acaatactgc aaaggcaatt
541 gcagaatggg gtctggatta tgttgtcctg acatctgtgg atcgagatga tatgcctgat
601 gggggagctg aacacattgc aaagaccgta tcatatttaa aggaaaggaa tccaaaaatc
661 cttgtggagt gtcttactcc tgattttcga ggtgatctca aagcaataga aaaagttgct
721 ctgtcaggat tagatgtgta tgcacataat gtagaaacag tcccggaatt acagagtaag
781 gttcgtgatc ctcgggtcaa ttttgatcag tccctacgtg tactgaaaca tgccaagaag
841 gttcagcctg atgttatttc taaaacatct ataatgttgg gtttaggcga gaatgatgag
901 caagtatatg caacaatgaa agcacttcgt gaggcagatg tagactgctt gactttagga
961 caatatatgc agccaacaag gcgtcacctt aaggttgaag aatatattac tcctgaaaaa
1021 ttcaaatact gggaaaaagt aggaaatgaa cttggatttc attatactgc aagtggccct
1081 ttggtgcgtt cttcatataa agcaggtgaa tttttcctga aaaatctagt ggctaaaaga
1141 aaaacaaaag acctctaaaa cttcaacaag accttcaaga tcacagaaat ttttaaaatt
1201 tgattccagt taataacaga ggtggtgcca gaatgcctgg actgcagtgg atgtacccca
1261 cctctttgct taaaaaaaaa aaaaaaa
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