LOCUS BC023558 2143 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens sparc/osteonectin, cwcv and kazal-like domains
proteoglycan (testican) 2, mRNA (cDNA clone MGC:19496
IMAGE:4137728), complete cds.
ACCESSION BC023558
VERSION BC023558.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2143)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2143)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC023558.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 27 Row: n Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7662035.
FEATURES Location/Qualifiers
source 1..2143
/db_xref="H-InvDB:HIT000050828"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:19496 IMAGE:4137728"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2143
/gene="SPOCK2"
/gene_synonym="testican-2"
/db_xref="GeneID:9806"
/db_xref="HGNC:HGNC:13564"
/db_xref="MIM:607988"
CDS 440..1714
/gene="SPOCK2"
/gene_synonym="testican-2"
/codon_start=1
/product="SPOCK2 protein"
/protein_id="AAH23558.1"
/db_xref="GeneID:9806"
/db_xref="HGNC:HGNC:13564"
/db_xref="MIM:607988"
/translation="MRAPGCGRLVLPLLLLAAAALAEGDAKGLKEGETPGNFMEDEQW
LSSISQYSGKIKHWNRFRDEVEDDYIKSWEDNQQGDEALDTTKDPCQKVKCSRHKVCI
AQGYQRAMCISRKKLEHRIKQPTVKLHGNKDSICKPCHMAQLASVCGSDGHTYSSVCK
LEQQACLSSKQLAVRCEGPCPCPTEQAATSTADGKPETCTGQDLADLGDRLRDWFQLL
HENSKQNGSASSVAGPASGLDKSLGASCKDSIGWMFSKLDTSADLFLDQTELAAINLD
KYEVCIRPFFNSCDTYKDGRVSTAEWCFCFWREKPPCLAELERIQIQEAAKKKPGIFI
PSCDEDGYYRKMQCDQSSGDCWCVDQLGLELTGTRTHGSPDCDDIVGFSGDFGSGVGW
EDEEEKETEEAGEEAEEEEGEAGEADDGGYIW"
BASE COUNT 506 a 584 c 665 g 388 t
ORIGIN
1 cagggacatt tcgggattct ggatttcttg gtctgcaact ccctgtgtgc caggcggggc
61 tgggccccgc tgcggctggc tggcgtttgg cgctctcatc gagggagcgc ttcgctttga
121 aatctaccca ctcctttttt tttttttaaa ccaggtatga tgatgtcaaa cgtgatgctg
181 atgctacagt tacagcccct gctggcgcag cctctctgat tctctctccc tctccgcgtc
241 cagtgctggg ctttttcaga caagtgcatc tcctaaccag gtcacatttc agccgcgacc
301 cactctccgc cagtcaccgg aggcagaccg cgggaggaga gctgaggaca gccgcgtgcg
361 cttcgccagc agcggggtgg gaggaaggac attaaaatac tgcagaagtc aagacccccc
421 aggtcgaacc cagaccacga tgcgcgcccc aggctgcggg cggctggtgc tgccgctgct
481 gctcctggcc gcggcagccc tggccgaagg cgacgccaag gggctcaagg agggcgagac
541 ccccggcaat ttcatggagg acgagcaatg gctgtcgtcc atctcgcagt acagcggcaa
601 gatcaagcac tggaaccgct tccgagacga agtggaggat gactatatca agagctggga
661 ggacaatcag caaggagatg aagccctgga taccaccaag gacccctgcc agaaggtgaa
721 gtgcagccgc cacaaggtgt gcattgccca gggctaccag cgggccatgt gcatcagtcg
781 caagaagctg gagcacagga tcaagcagcc gaccgtgaaa ctccatggaa acaaagactc
841 catctgcaag ccctgccaca tggcccagct tgcctctgtc tgcggctcag atggccacac
901 ttacagctct gtgtgtaagc tggagcaaca ggcgtgcctg agcagcaagc agctggcggt
961 gcgatgcgag ggcccctgcc cctgccccac ggagcaggct gccacctcca ccgccgatgg
1021 caaaccagag acttgcaccg gtcaggacct ggctgacctg ggagatcggc tgcgggactg
1081 gttccagctc cttcatgaga actccaagca gaatggctca gccagcagtg tagccggccc
1141 ggccagcggg ctggacaaga gcctgggggc cagctgcaag gactccattg gctggatgtt
1201 ctccaagctg gacaccagtg ctgacctctt cctggaccag acggagctgg ccgccatcaa
1261 cctggacaag tacgaggtct gcatccgtcc cttcttcaac tcctgtgaca cctacaagga
1321 tggccgggtc tctactgctg agtggtgctt ctgcttctgg agggagaagc ccccctgcct
1381 ggcagagctg gagcgcatcc agatccagga ggccgccaag aagaagccag gcatcttcat
1441 cccgagctgc gacgaggatg gctactaccg gaagatgcag tgtgaccaga gcagcggtga
1501 ctgctggtgt gtggaccagc tgggcctgga gctgactggc acgcgcacgc atgggagccc
1561 cgactgcgat gacatcgtgg gcttctcggg ggactttgga agcggtgtcg gctgggagga
1621 tgaggaggag aaggagacgg aggaagcagg cgaggaggcc gaggaggagg agggcgaggc
1681 aggcgaggct gacgacgggg gctacatctg gtagacgccc tcaggagccg gctgccgggg
1741 gggactcaac agcagagctc tgagcagcag caggcaactt cgagaacgga tccagaaatg
1801 cagtcagaag gaccctgctc cacctggggg gactgggagt gtgagtgtgc atggcatgtg
1861 tgtggcacag atggctggga cgggtgacgg tgtgagtgca tgtgtgcatg catgtgtgta
1921 tgtgtgtgtg tgtgtggcat gcgctgacaa atgtgtcctt gatccacact gctcctggca
1981 gagtgagtca cccaaaggcc ccttcggcct ccttgtagct gttttctttc cttttgttgt
2041 tggttttaaa atacattcac acacaaatac aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2101 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//