LOCUS BC023523 2681 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens diacylglycerol kinase, alpha 80kDa, mRNA (cDNA clone
MGC:12821 IMAGE:4304187), complete cds.
ACCESSION BC023523
VERSION BC023523.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2681)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2681)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC023523.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 17 Row: p Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41872493.
FEATURES Location/Qualifiers
source 1..2681
/db_xref="H-InvDB:HIT000050809"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:12821 IMAGE:4304187"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_46"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2681
/gene="DGKA"
/gene_synonym="DGK-alpha"
/gene_synonym="MGC12821"
/gene_synonym="MGC42356"
/db_xref="GeneID:1606"
/db_xref="HGNC:HGNC:2849"
/db_xref="MIM:125855"
CDS 201..2408
/gene="DGKA"
/gene_synonym="DGK-alpha"
/gene_synonym="MGC12821"
/gene_synonym="MGC42356"
/codon_start=1
/product="diacylglycerol kinase, alpha 80kDa"
/protein_id="AAH23523.1"
/db_xref="GeneID:1606"
/db_xref="HGNC:HGNC:2849"
/db_xref="MIM:125855"
/translation="MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQ
GDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCY
FSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPIL
QEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVY
CNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGG
CESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSI
YPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQR
VLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANL
PVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQ
TEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSES
IFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYG
INQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKC
SEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS"
BASE COUNT 721 a 669 c 665 g 626 t
ORIGIN
1 gcgagccctc tcaagcaaga tataacttcc ccaagtcaca cagtggtatc agagctaaga
61 atgggaccca gatatgactg atctagttct gttccaaaac cgtgctgtat tatattaacg
121 cctaccctct gaagaggtcc aagcaacgga agtactacta cgaagctgcc tttctggcca
181 tccttgagaa aaatagacag atggccaagg agaggggcct aataagcccc agtgattttg
241 cccagctgca aaaatacatg gaatactcca ccaaaaaggt cagtgatgtc ctaaagctct
301 tcgaggatgg cgagatggct aaatatgtcc aaggagatgc cattgggtac gagggattcc
361 agcaattcct gaaaatctat ctcgaagtgg ataatgttcc cagacaccta agcctggcac
421 tgtttcaatc ctttgagact ggtcactgct taaatgagac aaatgtgaca aaagatgtgg
481 tgtgtctcaa tgatgtttcc tgctactttt cccttctgga gggtggtcgg ccagaagaca
541 agttagaatt caccttcaag ctgtacgaca cggacagaaa tgggatcctg gacagctcag
601 aagtggacaa aattatccta cagatgatgc gagtggctga atacctggat tgggatgtgt
661 ctgagctgag gccgattctt caggagatga tgaaagagat tgactatgat ggcagtggct
721 ctgtctctca agctgagtgg gtccgggctg gggccaccac cgtgccactg ctagtgctgc
781 tgggtctgga gatgactctg aaggacgacg gacagcacat gtggaggccc aagaggttcc
841 ccagaccagt ctactgcaat ctgtgcgagt caagcattgg tcttggcaaa cagggactga
901 gctgtaacct ctgtaagtac actgttcacg accagtgtgc catgaaagcc ctgccttgtg
961 aagtcagcac ctatgccaag tctcggaagg acattggtgt ccaatcacat gtgtgggtgc
1021 gaggaggctg tgagtccggg cgctgcgacc gctgtcagaa aaagatccgg atctaccaca
1081 gtctgaccgg gctgcattgt gtatggtgcc acctagagat ccacgatgac tgcctgcaag
1141 cggtgggcca tgagtgtgac tgtgggctgc tccgggatca catcctgcct ccatcttcca
1201 tctatcccag tgtcctggcc tctggaccgg atcgtaaaaa tagcaaaaca agccagaaga
1261 ccatggatga tttaaatttg agcacctctg aggctctgcg gattgaccct gttcctaaca
1321 cccacccact tctcgtcttt gtcaatccta agagtggcgg gaagcagggg cagagggtgc
1381 tctggaagtt ccagtatata ttaaaccctc gacaggtgtt caacctccta aaggatggtc
1441 ctgagatagg gctccgatta ttcaaggatg ttcctgatag ccggattttg gtgtgtggtg
1501 gagacggcac agtaggctgg attctagaga ccattgacaa agctaacttg ccagttttgc
1561 ctcctgttgc tgtgttgccc ctgggtactg gaaatgatct ggctcgatgc ctaagatggg
1621 gaggaggtta tgaaggacag aatctggcaa agatcctcaa ggatttagag atgagtaaag
1681 tggtacatat ggatcgatgg tctgtggagg tgatacctca acaaactgaa gaaaaaagtg
1741 acccagtccc ctttcaaatc atcaataact acttctctat tggcgtggat gcctctattg
1801 ctcatcgatt ccacatcatg cgagagaaat atccggagaa gttcaacagc agaatgaaga
1861 acaagctatg gtacttcgaa tttgccacat ctgaatccat cttctcaaca tgcaaaaagc
1921 tggaggagtc tttgacagtt gagatctgtg ggaaaccgct ggatctgagc aacctgtccc
1981 tagaaggcat cgcagtgcta aacatcccta gcatgcatgg tggctccaac ctctggggtg
2041 ataccaggag accccatggg gatatctatg ggatcaacca ggccttaggt gctacagcta
2101 aagtcatcac cgaccctgat atcctgaaaa cctgtgtacc agacctaagt gacaagagac
2161 tggaagtggt tgggctggag ggtgcaattg agatgggcca aatctatacc aagctcaaga
2221 atgctggacg tcggctggcc aagtgctctg agatcacctt ccacaccaca aaaacccttc
2281 ccatgcaaat tgacggagaa ccctggatgc agacgccctg tacaatcaag atcacccaca
2341 agaaccagat gcccatgctc atgggcccac ccccccgctc caccaatttc tttggcttct
2401 tgagctaagg gggacaccct tggcctccaa gccagccttg aacccacctc cctgtccctg
2461 gactctactc ccgaggctct gtacattgct gccacatact cctgccagct tgggggagtg
2521 ttccttcacc ctcacagtat ttattatcct gcaccacctc actgttcccc atgcgcacac
2581 acatacacac accccaaaac acatacattg aaagtgcctc atctgaataa aatgacttgt
2641 gtttcccctt tgggatctgc taaaaaaaaa aaaaaaaaaa a
//