LOCUS BC023234 1747 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphoglycerate kinase 1, mRNA (cDNA clone MGC:8947
IMAGE:3917985), complete cds.
ACCESSION BC023234
VERSION BC023234.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1747)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1747)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (04-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 26 Row: a Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 5453993.
FEATURES Location/Qualifiers
source 1..1747
/db_xref="H-InvDB:HIT000090746"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8947 IMAGE:3917985"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1747
/gene="PGK1"
/gene_synonym="MIG10"
/gene_synonym="PGKA"
/db_xref="GeneID:5230"
/db_xref="HGNC:HGNC:8896"
/db_xref="MIM:311800"
CDS 37..1290
/gene="PGK1"
/gene_synonym="MIG10"
/gene_synonym="PGKA"
/codon_start=1
/product="phosphoglycerate kinase 1"
/protein_id="AAH23234.1"
/db_xref="GeneID:5230"
/db_xref="HGNC:HGNC:8896"
/db_xref="MIM:311800"
/translation="MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAV
PSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGP
EVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDV
YVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVA
DKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKN
GVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQI
VWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHV
STGGGASLELLEGKVLPGVDALSNI"
BASE COUNT 473 a 374 c 444 g 456 t
ORIGIN
1 gaatcaccga cctctctccc cagctgtatt tccaaaatgt cgctttctaa caagctgacg
61 ctggacaagc tggacgttaa agggaagcgg gtcgttatga gagtcgactt caatgttcct
121 atgaagaaca accagataac aaacaaccag aggattaagg ctgctgtccc aagcatcaaa
181 ttctgcttgg acaatggagc caagtcggta gtccttatga gccacctagg ccggcctgat
241 ggtgtgccca tgcctgacaa gtactcctta gagccagttg ctgtagaact caaatctctg
301 ctgggcaagg atgttctgtt cttgaaggac tgtgtaggcc cagaagtgga gaaagcctgt
361 gccaacccag ctgctgggtc tgtcatcctg ctggagaacc tccgctttca tgtggaggaa
421 gaagggaagg gaaaagatgc ttctgggaac aaggttaaag ccgagccagc caaaatagaa
481 gctttccgag cttcactttc caagctaggg gatgtctatg tcaatgatgc ttttggcact
541 gctcacagag cccacagctc catggtagga gtcaatctgc cacagaaggc tggtgggttt
601 ttgatgaaga aggagctgaa ctactttgca aaggccttgg agagcccaga gcgacccttc
661 ctggccatcc tgggcggagc taaagttgca gacaagatcc agctcatcaa taatatgctg
721 gacaaagtca atgagatgat tattggtggt ggaatggctt ttaccttcct taaggtgctc
781 aacaacatgg agattggcac ttctctgttt gatgaagagg gagccaagat tgtcaaagac
841 ctaatgtcca aagctgagaa gaatggtgtg aagattacct tgcctgttga ctttgtcact
901 gctgacaagt ttgatgagaa tgccaagact ggccaagcca ctgtggcttc tggcatacct
961 gctggctgga tgggcttgga ctgtggtcct gaaagcagca agaagtatgc tgaggctgtc
1021 actcgggcta agcagattgt gtggaatggt cctgtggggg tatttgaatg ggaagctttt
1081 gcccggggaa ccaaagctct catggatgag gtggtgaaag ccacttctag gggctgcatc
1141 accatcatag gtggtggaga cactgccact tgctgtgcca aatggaacac ggaggataaa
1201 gtcagccatg tgagcactgg gggtggtgcc agtttggagc tcctggaagg taaagtcctt
1261 cctggggtgg atgctctcag caatatttag tactttcctg ccttttagtt cctgtgcaca
1321 gcccctaagt caacttagca ttttctgcat ctccacttgg cattagctaa aaccttccat
1381 gtcaagattc agctagtggc caagagatgc agtgccagga acccttaaac agttgcacag
1441 catctcagct catcttcact gcaccctgga tttgcataca ttcttcaaga tcccatttga
1501 attttttagt gactaaacca ttgtgcattc tagagtgcat atatttatat tttgcctgtt
1561 aaaaagaaag tgagcagtgt tagcttagtt ctcttttgat gtaggttatt atgattagct
1621 ttgtcactgt ttcactactc agcatggaaa caagatgaaa ttccatttgt aggtagtgag
1681 acaaaattga tgatccatta agtaaacaat aaaagtgtcc attgaaaccg tgaaaaaaaa
1741 aaaaaaa
//