LOCUS BC022804 976 bp mRNA linear HUM 10-DEC-2007
DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2K (UBC1 homolog,
yeast), mRNA (cDNA clone MGC:39129 IMAGE:4893778), complete cds.
ACCESSION BC022804
VERSION BC022804.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 976)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 976)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (04-FEB-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 42 Row: j Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21536483.
FEATURES Location/Qualifiers
source 1..976
/db_xref="H-InvDB:HIT000039642"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:39129 IMAGE:4893778"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..976
/gene="UBE2K"
/gene_synonym="E2-25K"
/gene_synonym="HIP2"
/gene_synonym="HYPG"
/gene_synonym="LIG"
/db_xref="GeneID:3093"
/db_xref="HGNC:HGNC:4914"
/db_xref="MIM:602846"
CDS 161..763
/gene="UBE2K"
/gene_synonym="E2-25K"
/gene_synonym="HIP2"
/gene_synonym="HYPG"
/gene_synonym="LIG"
/codon_start=1
/product="ubiquitin-conjugating enzyme E2K (UBC1 homolog,
yeast)"
/protein_id="AAH22804.1"
/db_xref="GeneID:3093"
/db_xref="HGNC:HGNC:4914"
/db_xref="MIM:602846"
/translation="MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIA
GPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAA
AMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSP
EYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN"
BASE COUNT 310 a 184 c 256 g 226 t
ORIGIN
1 cacactgagg cgagcgcggc ggccggggtg gtagtggcag tgttcgtgtg ctcaggtctg
61 aatcgccgag ggaggaggcg gtggaggaag aggtggcggc ggtggcggtg gtcgtagcgg
121 tggcggagga ggcgggtacg aatcagctgc gggcggagac atggccaaca tcgcggtgca
181 gcgaatcaag cgggagttca aggaggtgct gaagagcgag gagacgagca aaaatcaaat
241 taaagtagat cttgtagatg agaattttac agaattaaga ggagaaatag caggacctcc
301 agacacacca tatgaaggag gaagatacca actagagata aaaataccag aaacataccc
361 atttaatccc cctaaggtcc ggtttatcac taaaatatgg catcctaata ttagttccgt
421 cacaggggct atttgtttgg atatcctgaa agatcaatgg gcagctgcaa tgactctccg
481 cacggtatta ttgtcattgc aagcactatt ggcagctgca gagccagatg atccacagga
541 tgctgtagta gcaaatcagt acaaacaaaa tcccgaaatg ttcaaacaga cagctcgact
601 ttgggcacat gtgtatgctg gagcaccagt ttctagtcca gaatacacca aaaaaataga
661 aaacctatgt gctatgggct ttgataggaa tgcagtaata gtggccttgt cttcaaaatc
721 atgggatgta gagactgcaa cagaattgct tctgagtaac tgaggcatag agagctgctg
781 atatagtcaa gcttgcctct tcttgaggag caccaacatc tgttattttt aggattctgc
841 atagatttct tttaaactgg cattcttgcc taatgatgtt atctaggcac cattggagac
901 tgaaaaaaaa aaatccctgc tctgtaaata aagctaatta aacgtctgtg taaatttaaa
961 aaaaaaaaaa aaaaaa
//