LOCUS BC021576 2005 bp mRNA linear HUM 15-FEB-2005
DEFINITION Homo sapiens Sfi1 homolog, spindle assembly associated (yeast),
mRNA (cDNA clone IMAGE:3936711), partial cds.
ACCESSION BC021576
VERSION BC021576.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2005)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2005)
AUTHORS Director MGC Project.
TITLE Direct Submission
JOURNAL Submitted (14-JAN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 40 Row: b Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..2005
/db_xref="H-InvDB:HIT000090426"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3936711"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2005
/gene="SFI1"
/gene_synonym="hSfi1p"
/gene_synonym="PISD"
/db_xref="GeneID:9814"
CDS <1..1825
/gene="SFI1"
/gene_synonym="hSfi1p"
/gene_synonym="PISD"
/codon_start=2
/product="SFI1 protein"
/protein_id="AAH21576.1"
/db_xref="GeneID:9814"
/translation="RQKLMRADLHHQHSVLHRALQAWVTYQGRVRSILREVAARESQH
NRQLLRGALRRWKENTMARVDEAKKTFQASTHYRRTICSKVLVQWREAVSVQMYYRQQ
EDCAIWEAQKVLDRGCLRTWFQRWWDCSRRSAQQRLQLERAVQHHHRQLLLEGLARWK
THHLQCVRKRLLHRQSTQLLAQRLSRTCFRQWRQQLAARRQEQRATVRALWFWAFSLQ
AKVWATWLAFVLERRRKKARLQWALQAYQGQLLQEGATRLLRFAASMKASRQQLQAQQ
QVQAAHSLHRAVRRCATLWKQKVLGRGGKPQPLAAIAPSRKVTFEGPLLNRIAAGAGD
GTLETKRPQASRPLGALGRLAAEEPHALELNTAHSARKQPRRPHFLLEPAQSQRPQKP
QEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLL
PLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLST
AGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQ
VQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC"
BASE COUNT 452 a 610 c 633 g 310 t
ORIGIN
1 gcggcagaag ctgatgcgag cagacctgca ccaccagcac agcgtgctgc acagggcgct
61 gcaggcatgg gtgacttacc agggcagggt gcgaagcatc ctccgggagg tggcagccag
121 ggagagccag cacaacaggc agctgctgcg cggggcatta cgtcgctgga aagagaacac
181 catggcccga gtggatgaag ccaaaaaaac ctttcaagca agtactcatt acagaaggac
241 catatgttcc aaggtcctgg tccagtggcg ggaagctgtg tcagtgcaga tgtattaccg
301 acagcaggag gactgtgcca tctgggaggc ccagaaggtg ctggacaggg gctgtctgcg
361 gacctggttt cagcgctggt gggactgcag ccggaggtca gcccagcaga gactgcagct
421 ggagagggca gtgcaacacc accaccggca gctgctgctg gaggggctgg cccggtggaa
481 gacgcaccat ctgcagtgtg tcaggaagag gctcctgcac aggcagagca cccaactgct
541 ggcacagaga ctcagccgga cctgcttccg ccagtggaga caacagctgg cagccaggag
601 gcaggagcag cgggcgacag tgcgggccct gtggttctgg gccttctcgc tgcaggcaaa
661 ggtgtgggcc acgtggctgg cctttgtact ggaaaggagg agaaagaagg cgcggctgca
721 gtgggcgctc caggcctacc aggggcagct cctccaggag ggtgccacgc ggctcctgcg
781 ctttgcagcc agcatgaagg cctcccggca gcagctgcag gcccagcagc aggtccaggc
841 ggctcacagt ctccatcgtg ccgtccgccg ctgtgccacg ctctggaaac agaaagtgct
901 gggccggggc gggaagcctc agcccctggc agccatcgca cccagcagga aagtgacgtt
961 tgagggtccc cttctcaacc gcattgctgc tggggctggg gatggcaccc ttgagaccaa
1021 gaggccacag gcttctcggc ctctgggagc tctgggccgc ctggctgctg aggagcccca
1081 cgccctggag ctcaacactg cccactcagc gaggaagcag ccgcgacgcc cacacttcct
1141 gttggagcct gcgcagagcc agaggcctca gaagccacag gaacatggcc taggcatggc
1201 tcagccagca gccccctccc tgacgcggcc cttcctggca gaggccccga cagcactggt
1261 cccacacagc cccctgcctg gggccctgtc aagcgcccct ggcccgaagc agcccccgac
1321 ggcaagcaca ggcccggagc tgctgctgct gcctctttcc tccttcatgc cctgcggggc
1381 ggctgcacca gccagggtgt cagcacagcg ggctactcct agggataagc ccccggtccc
1441 ctcatccctg gccagtgtcc ctgaccccca tctactcctt cctggggact tctcagccac
1501 cagggctggg cctggacttt caactgcagg cagcctggac cttgaggctg aacttgagga
1561 gatccagcag caactactgc actaccagac caccaagcag aacctctggt cctgtcggcg
1621 gcaagcgagc agcctgcgca ggtggctgga gctgaacaga gaggagccgg ggcctgagga
1681 ccaggaagta gagcagcagg tgcagaaaga gctggaacag gtggaaatgc agatccagct
1741 gctggcagag gagctccagg ctcagcgcca gcccattggc gcctgcgttg cccgcatcca
1801 ggccctgcgg caggccctgt gctagcgtgt tcgcaccagg aacgcaggtg ctgggctgtc
1861 ggggaggcct caggccacct ccaggaacag aacacagttt taagtttgat tttttttatt
1921 tcaaaatgct ttgcaattaa atgaattact gttcaaaaaa aaaaaaaaaa aaaaaaaaaa
1981 aaaaaaaaaa aaaaaaaaaa aaaaa
//