LOCUS BC020861 1728 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens amylase, alpha 2B (pancreatic), mRNA (cDNA clone
MGC:24057 IMAGE:4551745), complete cds.
ACCESSION BC020861
VERSION BC020861.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1728)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1728)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-JAN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 36 Row: e Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21359830.
FEATURES Location/Qualifiers
source 1..1728
/db_xref="H-InvDB:HIT000039006"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:24057 IMAGE:4551745"
/tissue_type="Kidney"
/clone_lib="NIH_MGC_75"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1728
/gene="AMY2B"
/db_xref="GeneID:280"
/db_xref="HGNC:HGNC:478"
/db_xref="MIM:104660"
CDS 137..1672
/gene="AMY2B"
/codon_start=1
/product="amylase, alpha 2B (pancreatic)"
/protein_id="AAH20861.1"
/db_xref="GeneID:280"
/db_xref="HGNC:HGNC:478"
/db_xref="MIM:104660"
/translation="MKFFLLLFTIGFCWAQYSPNTQQGRTSIVHLFEWRWVDIALECE
RYLAPKGFGGVQVSPPNENVAIHNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCN
NVGVRIYVDAVINHMSGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGS
GDIENYNDATQVRDCRLVGLLDLALEKDYVRSKIAEYMNHLIDIGVAGFRLDASKHMW
PGDIKAILDKLHNLNSNWFPAGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAK
LGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDAR
LYKMAVGFMLAHPYGFTRVMSSYRWPRQFQNGNDVNDWVGPPNNNGVIKEVTINPDTT
CGNDWVCEHRWRQIRNMVNFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWT
FSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAE
SKL"
BASE COUNT 532 a 300 c 399 g 497 t
ORIGIN
1 cacatcactc aactactatt agctggcgag tcaactcacg ttgttggaat gactagggac
61 aactagactt caagtgatct ttcatcccag gaatataaat agtttctgga aaggacactg
121 acaacttcaa agcaaaatga agttctttct gttgcttttc accattgggt tctgctgggc
181 tcagtattcc ccaaatacac aacaaggacg gacatctatt gttcatctgt ttgaatggcg
241 atgggttgat attgctcttg aatgtgagcg atatttagct cccaagggat ttggaggggt
301 tcaggtctct ccaccaaatg aaaatgttgc aattcacaac cctttcagac cttggtggga
361 aagataccaa ccagttagct ataaattatg cacaagatct ggaaatgaag atgaatttag
421 aaacatggtg actagatgta acaatgttgg ggttcgtatt tatgtggatg ctgtaattaa
481 tcatatgtct ggtaatgctg tgagtgcagg aacaagcagt acctgtggaa gttacttcaa
541 ccctggaagt agggactttc cagcagtccc atattctgga tgggatttta atgatggtaa
601 atgtaaaact ggaagtggag atatcgagaa ctacaatgat gctactcagg tcagagattg
661 tcgtctggtt ggtcttcttg atcttgcact ggagaaagat tatgtgcgtt ccaagattgc
721 cgaatatatg aaccatctca ttgacattgg tgttgcaggg ttcagacttg atgcttccaa
781 gcacatgtgg cctggagaca taaaggcaat tttggacaaa ctgcataatc taaacagtaa
841 ctggttccct gcaggaagta aacctttcat ttaccaggag gtaattgatc tgggtggtga
901 gccaattaaa agcagtgact actttggaaa tggccgggtg acagaattca agtatggtgc
961 aaaactcggc acagttattc gcaagtggaa tggagagaag atgtcttacc taaagaactg
1021 gggagaaggt tggggtttca tgccttctga cagagcactt gtctttgtgg ataaccatga
1081 caatcaacga ggacatgggg ctggaggagc ctctattctt accttctggg atgctaggct
1141 gtataaaatg gcagttggat ttatgcttgc tcatccttat ggttttacac gagtaatgtc
1201 aagctaccgt tggccaagac agtttcaaaa tggaaacgat gttaatgatt gggttgggcc
1261 accaaataat aatggagtaa ttaaagaagt tactattaat ccagacacta cttgtggcaa
1321 tgactgggtc tgtgaacatc gatggcgcca aataaggaac atggttaatt tccgcaatgt
1381 agtggatggc cagcctttta caaactggta tgataatggg agcaaccaag tggcttttgg
1441 gagaggaaac agaggattca ttgttttcaa caatgatgac tggacatttt ctttaacttt
1501 gcaaactggt cttcctgctg gcacatactg tgatgtcatt tctggagata aaattaatgg
1561 caattgcaca ggcattaaaa tctacgtttc tgacgatggc aaagctcatt tttctattag
1621 taactctgct gaggatccat ttattgcaat tcatgctgaa tctaaattat aaaatttaaa
1681 attaaatgca tatcctcaag aaaaaaaaaa aaaaaaaaaa aaaaaaaa
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