LOCUS BC019864 2429 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphodiesterase 4A, cAMP-specific (phosphodiesterase
E2 dunce homolog, Drosophila), mRNA (cDNA clone MGC:30168
IMAGE:4942336), complete cds.
ACCESSION BC019864
VERSION BC019864.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2429)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2429)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (19-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 42 Row: i Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..2429
/db_xref="H-InvDB:HIT000038699"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:30168 IMAGE:4942336"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2429
/gene="PDE4A"
/gene_synonym="PDE4"
/db_xref="GeneID:5141"
/db_xref="HGNC:HGNC:8780"
/db_xref="MIM:600126"
CDS 185..2128
/gene="PDE4A"
/gene_synonym="PDE4"
/codon_start=1
/product="phosphodiesterase 4A, cAMP-specific
(phosphodiesterase E2 dunce homolog, Drosophila)"
/protein_id="AAH19864.1"
/db_xref="GeneID:5141"
/db_xref="HGNC:HGNC:8780"
/db_xref="MIM:600126"
/translation="MPLVDFFCETCSKPWLVGWWDQFKRMLNRELTHLSEMSRSGNQV
SEYISTTFLDKQNEVEIPSPTMKEREKQQAPRPRPSQPPPPPVPHLQPMSQITGLKKL
MHSNSLNNSNIPRFGVKTDQEELLAQELENLNKWGLNIFCVSDYAGGRSLTCIMYMIF
QERDLLKKFRIPVDTMVTYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAV
FTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQ
EDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLL
DNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMC
DKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSP
PPEEESRGPGHPPLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGLSGVEEALD
ATIAWEASPAQESLEVMAQEASLEAELEAVYLTQQAQSTGSAPVAPDEFSSREEFVVA
VSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGLPSTAAEVEAQREHQAAKRACSACA
GTFGEDTSALPAPGGGGSGGDPT"
BASE COUNT 550 a 753 c 659 g 467 t
ORIGIN
1 ggctgctgct gggagcgcgg aggggaaggg agcccccagc cctgctgggc cggcccaggc
61 ccctccgcgg ctcccccttc cactacccac ctgcccggca ccccctcccc agtggttgtt
121 aaccccggga ctccccaagc ccagcctctg tgtgcagcag ccccaggcgg gctaagtctc
181 caagatgccc ttggtggatt tcttctgcga gacctgctct aagccttggc tggtgggctg
241 gtgggaccag ttcaaaagga tgttgaaccg tgagctcaca cacctgtcag aaatgagcag
301 gtccggaaac caggtctcag agtacatttc cacaacattc ctggacaaac agaatgaagt
361 ggagatccca tcacccacga tgaaggaacg agaaaaacag caagcgccgc gaccaagacc
421 ctcccagccg cccccgcccc ctgtaccaca cttacagccc atgtcccaaa tcacagggtt
481 gaaaaagttg atgcatagta acagcctgaa caactctaac attccccgat ttggggtgaa
541 gaccgatcaa gaagagctcc tggcccaaga actggagaac ctgaacaagt ggggcctgaa
601 catcttttgc gtgtcggatt acgctggagg ccgctcactc acctgcatca tgtacatgat
661 attccaggag cgggacctgc tgaagaaatt ccgcatccct gtggacacga tggtgacata
721 catgctgacg ctggaggatc actaccacgc tgacgtggcc taccataaca gcctgcacgc
781 agctgacgtg ctgcagtcca cccacgtact gctggccacg cctgcactag atgcagtgtt
841 cacggacctg gagattctcg ccgccctctt cgcggctgcc atccacgatg tggatcaccc
901 tggggtctcc aaccagttcc tcatcaacac caattcggag ctggcgctca tgtacaacga
961 tgagtcggtg ctcgagaatc accacctggc cgtgggcttc aagctgctgc aggaggacaa
1021 ctgcgacatc ttccagaacc tcagcaagcg ccagcggcag agcctacgca agatggtcat
1081 cgacatggtg ctggccacgg acatgtccaa gcacatgacc ctcctggctg acctgaagac
1141 catggtggag accaagaaag tgaccagctc aggggtcctc ctgctagata actactccga
1201 ccgcatccag gtcctccgga acatggtgca ctgtgccgac ctcagcaacc ccaccaagcc
1261 gctggagctg taccgccagt ggacagaccg catcatggcc gagttcttcc agcagggtga
1321 ccgagagcgc gagcgtggca tggaaatcag ccccatgtgt gacaagcaca ctgcctccgt
1381 ggagaagtct caggtgggtt ttattgacta cattgtgcac ccattgtggg agacctgggc
1441 ggaccttgtc cacccagatg cccaggagat cttggacact ttggaggaca accgggactg
1501 gtactacagc gccatccggc agagcccatc tccgccaccc gaggaggagt caagggggcc
1561 aggccaccca cccctgcctg acaagttcca gtttgagctg acgctggagg aggaagagga
1621 ggaagaaata tcaatggccc agataccgtg cacagcccaa gaggcattga ctgcgcaggg
1681 attgtcagga gtcgaggaag ctctggatgc aaccatagcc tgggaggcat ccccggccca
1741 ggagtcgttg gaagttatgg cacaggaagc atccctggag gccgagctgg aggcagtgta
1801 tttgacacag caggcacagt ccacaggcag tgcacctgtg gctccggatg agttctcgtc
1861 ccgggaggaa ttcgtggttg ctgtaagcca cagcagcccc tctgccctgg ctcttcaaag
1921 cccccttctc cctgcttgga ggaccctgtc tgtttcagag catgccccgg gcctcccggg
1981 cctcccctcc acggcggccg aggtggaggc ccaacgagag caccaggctg ccaagagggc
2041 ttgcagtgcc tgcgcaggga catttgggga ggacacatcc gcactcccag ctcctggtgg
2101 cggggggtca ggtggagacc ctacctgatc cccagacctc tgtccctgtt cccctccact
2161 cctcccctca ctcccctgct cccccgacca cctcctcctc tgcctcaaag actcttgtcc
2221 tcttgtccct cctgagaaaa aaagaaaacg aaaagtgggg tttttttctg ttttcttttt
2281 ttcccctttc cccctgcccc cacccacggg gccttttttt ggaggtgggg gctggggaat
2341 gaggggctga ggtcccggaa gggattttat ttttttgaat tttaattgta acatttttag
2401 aaaaagaaca aaaaaaaaaa aaaaaaaaa
//