LOCUS BC019265 1744 bp mRNA linear HUM 16-SEP-2003
DEFINITION Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone
IMAGE:3503708), partial cds.
ACCESSION BC019265
VERSION BC019265.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1744)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1744)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (13-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Sep 16, 2003 this sequence version replaced BC019265.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield,
Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin,
Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo
Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven
Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline
Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott,
Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy,
George Yang, Scott Zuyderduyn, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 6 Row: j Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1744
/db_xref="H-InvDB:HIT000089895"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3503708"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..1744
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="MGC3932"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/db_xref="GeneID:5315"
/db_xref="MIM:179050"
CDS <1..1032
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="MGC3932"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/codon_start=1
/product="PKM2 protein"
/protein_id="AAH19265.2"
/db_xref="GeneID:5315"
/db_xref="MIM:179050"
/translation="GADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFG
VEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGI
MVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDV
ANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITS
DPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAH
LYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFT
NTMRVVPVP"
misc_feature 13..1026
/gene="PKM2"
/gene_synonym="CTHBP"
/gene_synonym="MGC3932"
/gene_synonym="OIP3"
/gene_synonym="PK3"
/gene_synonym="PKM"
/gene_synonym="TCB"
/gene_synonym="THBP1"
/note="PykF; Region: Pyruvate kinase [Carbohydrate
transport and metabolism]"
/db_xref="CDD:COG0469"
BASE COUNT 435 a 473 c 478 g 358 t
ORIGIN
1 ggtgccgact tcctggtgac ggaggtggaa aatggtggct ccttgggcag caagaagggt
61 gtgaaccttc ctggggctgc tgtggacttg cctgctgtgt cggagaagga catccaggat
121 ctgaagtttg gggtcgagca ggatgttgat atggtgtttg cgtcattcat ccgcaaggca
181 tctgatgtcc atgaagttag gaaggtcctg ggagagaagg gaaagaacat caagattatc
241 agcaaaatcg agaatcatga gggggttcgg aggtttgatg aaatcctgga ggccagtgat
301 gggatcatgg tggctcgtgg tgatctaggc attgagattc ctgcagagaa ggtcttcctt
361 gctcagaaga tgatgattgg acggtgcaac cgagctggga agcctgtcat ctgtgctact
421 cagatgctgg agagcatgat caagaagccc cgccccactc gggctgaagg cagtgatgtg
481 gccaatgcag tcctggatgg agccgactgc atcatgctgt ctggagaaac agccaaaggg
541 gactatcctc tggaggctgt gcgcatgcag cacctgattg cccgtgaggc agaggctgcc
601 atctaccact tgcaattatt tgaggaactc cgccgcctgg cgcccattac cagcgacccc
661 acagaagcca ccgccgtggg tgccgtggag gcctccttca agtgctgcag tggggccata
721 atcgtcctca ccaagtctgg caggtctgct caccaggtgg ccagataccg cccacgtgcc
781 cccatcattg ctgtgacccg gaatccccag acagctcgtc aggcccacct gtaccgtggc
841 atcttccctg tgctgtgcaa ggacccagtc caggaggcct gggctgagga cgtggacctc
901 cgggtgaact ttgccatgaa tgttggcaag gcccgaggct tcttcaagaa gggagatgtg
961 gtcattgtgc tgaccggatg gcgccctggc tccggcttca ccaacaccat gcgtgttgtt
1021 cctgtgccgt gatggacccc agagcccctc ctccagcccc tgtcccaccc ccttccccca
1081 gcccatccat taggccagca acgcttgtag aactcactct gggctgtaac gtggcactgg
1141 taggttggga caccagggaa gaagatcaac gcctcactga aacatggctg tgtttgcagc
1201 ctgctctagt gggacagccc agagcctggc tgcccatcat gtggccccac ccaatcaagg
1261 gaagaaggag gaatgctgga ctggaggccc ctggagccag atggcaagag ggtgacagct
1321 tcctttcctg tgtgtactct gtccagttcc tttagaaaaa atggatgccc agaggactcc
1381 caaccctggc ttggggtcaa gaaacagcca gcaagagtta ggggccttag ggcactgggc
1441 tgttgttcca ttgaagccga ctctggccct ggcccttact tgcttctcta gctctctagg
1501 cctctccagt ttgcacctgt ccccaccctc cactcagctg tcctgcagca aacactccac
1561 cctccacctt ccattttccc ccactactgc agcacctcca ggcctgttgc tatagagcct
1621 acctgtatgt caataaacaa cagctgaagc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1681 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaccctgg
1741 gggc
//