LOCUS BC019027 2447 bp mRNA linear HUM 04-AUG-2008
DEFINITION Homo sapiens X-ray repair complementing defective repair in Chinese
hamster cells 5 (double-strand-break rejoining), mRNA (cDNA clone
MGC:20677 IMAGE:4555162), complete cds.
ACCESSION BC019027
VERSION BC019027.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2447)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2447)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 19, 2003 this sequence version replaced BC019027.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 30 Row: h Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 12408650.
FEATURES Location/Qualifiers
source 1..2447
/db_xref="H-InvDB:HIT000038511"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20677 IMAGE:4555162"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_46"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2447
/gene="XRCC5"
/gene_synonym="KARP-1"
/gene_synonym="KARP1"
/gene_synonym="KU80"
/gene_synonym="Ku86"
/gene_synonym="KUB2"
/gene_synonym="NFIV"
/db_xref="GeneID:7520"
/db_xref="HGNC:HGNC:12833"
/db_xref="MIM:194364"
CDS 9..2207
/gene="XRCC5"
/gene_synonym="KARP-1"
/gene_synonym="KARP1"
/gene_synonym="KU80"
/gene_synonym="Ku86"
/gene_synonym="KUB2"
/gene_synonym="NFIV"
/codon_start=1
/product="X-ray repair complementing defective repair in
Chinese hamster cells 5 (double-strand-break rejoining)"
/protein_id="AAH19027.1"
/db_xref="GeneID:7520"
/db_xref="HGNC:HGNC:12833"
/db_xref="MIM:194364"
/translation="MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITMFVQR
QVFAENKDEIALVLFGTDGTDNPLSGGDQYQNITVHRHLMLPDFDLLEDIESKIQPGS
QQADFLDALIVSMDVIQHETIGKKFEKRHIEIFTDLSSRFSKSQLDIIIHSLKKCDIS
LQFFLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKGITEQQKEGLEIVKMVMISLEGE
DGLDEIYSFSESLRKLCVFKKIERHSIHWPCRLTIGSNLSIRIAAYKSILQERVKKTW
TVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQGFRYGSDIVPFSKVDEEQMKYK
SEGKCFSVLGFCKSSQVQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAI
VRYAYDKRANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTEAQ
LNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLPPI
QQHIWNMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQVTAQEIFQDNHEDGPTAKK
LKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFL
DTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDG
ITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI"
BASE COUNT 774 a 467 c 570 g 636 t
ORIGIN
1 ccggcaacat ggtgcggtcg gggaataagg cagctgttgt gctgtgtatg gacgtgggct
61 ttaccatgag taactccatt cctggtatag aatccccatt tgaacaagca aagaaggtga
121 taaccatgtt tgtacagcga caggtgtttg ctgagaacaa ggatgagatt gctttagtcc
181 tgtttggtac agatggcact gacaatcccc tttctggtgg ggatcagtat cagaacatca
241 cagtgcacag acatctgatg ctaccagatt ttgatttgct ggaggacatt gaaagcaaaa
301 tccaaccagg ttctcaacag gctgacttcc tggatgcact aatcgtgagc atggatgtga
361 ttcaacatga aacaatagga aagaagtttg agaagaggca tattgaaata ttcactgacc
421 tcagcagccg attcagcaaa agtcagctgg atattataat tcatagcttg aagaaatgtg
481 acatctccct gcaattcttc ttgcctttct cacttggcaa ggaagatgga agtggggaca
541 gaggagatgg cccctttcgc ttaggtggcc atgggccttc ctttccacta aaaggaatta
601 ccgaacagca aaaagaaggt cttgagatag tgaaaatggt gatgatatct ttagaaggtg
661 aagatgggtt ggatgaaatt tattcattca gtgagagtct gagaaaactg tgcgtcttca
721 agaaaattga gaggcattcc attcactggc cctgccgact gaccattggc tccaatttgt
781 ctataaggat tgcagcctat aaatcgattc tacaggagag agttaaaaag acttggacag
841 ttgtggatgc aaaaacccta aaaaaagaag atatacaaaa agaaacagtt tattgcttaa
901 atgatgatga tgaaactgaa gttttaaaag aggatattat tcaagggttc cgctatggaa
961 gtgatatagt tcctttctct aaagtggatg aggaacaaat gaaatataaa tcggagggga
1021 agtgcttctc tgttttggga ttttgtaaat cttctcaggt tcagagaaga ttcttcatgg
1081 gaaatcaagt tctaaaggtc tttgcagcaa gagatgatga ggcagctgca gttgcacttt
1141 cctccctgat tcatgctttg gatgacttag acatggtggc catagttcga tatgcttatg
1201 acaaaagagc taatcctcaa gtcggcgtgg cttttcctca tatcaagcat aactatgagt
1261 gtttagtgta tgtgcagctg cctttcatgg aagacttgcg gcaatacatg ttttcatcct
1321 tgaaaaacag taagaaatat gctcccaccg aggcacagtt gaatgctgtt gatgctttga
1381 ttgactccat gagcttggca aagaaagatg agaagacaga cacccttgaa gacttgtttc
1441 caaccaccaa aatcccaaat cctcgatttc agagattatt tcagtgtctg ctgcacagag
1501 ctttacatcc ccgggagcct ctacccccaa ttcagcagca tatttggaat atgctgaatc
1561 ctcccgctga ggtgacaacg aaaagtcaga ttcctctctc taaaataaag accctttttc
1621 ctctgattga agccaagaaa aaggatcaag tgactgctca ggaaattttc caagacaacc
1681 atgaagatgg acctacagct aaaaaattaa agactgagca agggggagcc cacttcagcg
1741 tctccagtct ggctgaaggc agtgtcacct ctgttggaag tgtgaatcct gctgaaaact
1801 tccgtgttct agtgaaacag aagaaggcca gctttgagga agcgagtaac cagctcataa
1861 atcacatcga acagtttttg gatactaatg aaacaccgta ttttatgaag agcatagact
1921 gcatccgagc cttccgggaa gaagccatta agttttcaga agagcagcgc tttaacaact
1981 tcctgaaagc ccttcaagag aaagtggaaa ttaaacaatt aaatcatttc tgggaaattg
2041 ttgtccagga tggaattact ctgatcacca aagaggaagc ctctggaagt tctgtcacag
2101 ctgaggaagc caaaaagttt ctggccccca aagacaaacc aagtggagac acagcagctg
2161 tatttgaaga aggtggtgat gtggacgatt tattggacat gatataggtc gtggatgtat
2221 ggggaatcta agagagctgc catcgctgtg atgctgggag ttctaacaaa acaagttgga
2281 tgcggccatt caaggggagc caaaatctca agaaattccc agcaggttac ctggaggcgg
2341 atcatctaat tctctgtgga atgaatacac acatatatat tacaagggat aatttagacc
2401 ccatacaagt ttataaagag tcattgttaa aaaaaaaaaa aaaaaaa
//