LOCUS BC018992 3137 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens glutamate-ammonia ligase (glutamine synthetase), mRNA
(cDNA clone MGC:20095 IMAGE:3352740), complete cds.
ACCESSION BC018992
VERSION BC018992.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3137)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3137)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 28 Row: b Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361767.
FEATURES Location/Qualifiers
source 1..3137
/db_xref="H-InvDB:HIT000038498"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20095 IMAGE:3352740"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_16"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3137
/gene="GLUL"
/gene_synonym="GS"
/gene_synonym="PIG43"
/db_xref="GeneID:2752"
/db_xref="HGNC:HGNC:4341"
/db_xref="MIM:138290"
CDS 461..1582
/gene="GLUL"
/gene_synonym="GS"
/gene_synonym="PIG43"
/codon_start=1
/product="glutamate-ammonia ligase (glutamine synthetase)"
/protein_id="AAH18992.1"
/db_xref="GeneID:2752"
/db_xref="HGNC:HGNC:4341"
/db_xref="MIM:138290"
/translation="MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKT
RTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVF
KYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGP
YYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHL
WVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEK
LSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKK
GYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN"
BASE COUNT 822 a 730 c 765 g 820 t
ORIGIN
1 gcggagcgtg tgagcagtac tgcggcctcc tctcctctcc taacctcgct ctcgcggcct
61 acctttaccc gcccgcctgc tcggcgacca gcggggatcc tcccccagcc gcaagtccac
121 gaagaaagca acgaatgaaa attatgaaga caacgagaag tcagactcct ccgggtcgcg
181 ctccagctgc ttcggcttcg tcgcctactc tgtgaactcc ggggagagat ctcgagtcaa
241 gattaagacc ttaacccacc aacctgcctg ttcggacacc ccccgggccg gccgctgtct
301 gtccccttct ccatcgccct ctcccagaaa gctccggtgc ttggaccagc tagagtctga
361 gaaagaggag aggcgcgaac gccactccaa aaagagaagg gttaaagagg gcaaccctaa
421 cgatacgctt gactttctgt ggctgggaac accttccacc atgaccacct cagcaagttc
481 ccacttaaat aaaggcatca agcaggtgta catgtccctg cctcagggtg agaaagtcca
541 ggccatgtat atctggatcg atggtactgg agaaggactg cgctgcaaga cccggaccct
601 ggacagtgag cccaagtgtg tggaagagtt gcctgagtgg aatttcgatg gctccagtac
661 tttacagtct gagggttcca acagtgacat gtatctcgtg cctgctgcca tgtttcggga
721 ccccttccgt aaggacccta acaagctggt gttatgtgaa gttttcaagt acaatcgaag
781 gcctgcagag accaatttga ggcacacctg taaacggata atggacatgg tgagcaacca
841 gcacccctgg tttggcatgg agcaggagta taccctcatg gggacagatg ggcacccctt
901 tggttggcct tccaacggct tcccagggcc ccagggtcca tattactgtg gtgtgggagc
961 agacagagcc tatggcaggg acatcgtgga ggcccattac cgggcctgct tgtatgctgg
1021 agtcaagatt gcggggacta atgccgaggt catgcctgcc cagtgggaat ttcagattgg
1081 accttgtgaa ggaatcagca tgggagatca tctctgggtg gcccgtttca tcttgcatcg
1141 tgtgtgtgaa gactttggag tgatagcaac ctttgatcct aagcccattc ctgggaactg
1201 gaatggtgca ggctgccata ccaacttcag caccaaggcc atgcgggagg agaatggtct
1261 gaagtacatc gaggaggcca ttgagaaact aagcaagcgg caccagtacc acatccgtgc
1321 ctatgatccc aagggaggcc tggacaatgc ccgacgtcta actggattcc atgaaacctc
1381 caacatcaac gacttttctg ctggtgtagc caatcgtagc gccagcatac gcattccccg
1441 gactgttggc caggagaaga agggttactt tgaagatcgt cgcccctctg ccaactgcga
1501 ccccttttcg gtgacagaag ccctcatccg cacgtgtctt ctcaatgaaa ccggcgatga
1561 gcccttccag tacaaaaatt aagtggacta gacctccagc tgttgagccc ctcctagttc
1621 ttcatcccac tccaactctt ccccctctcc cagttgtccc gattgtaact caaagggtgg
1681 aatatcaagg tcgttttttt cattccatgt gcccagttaa tcttgctttc tttgtttggc
1741 tgggatagag gggtcaagtt attaatttct tcacacctac cctccttttt ttccctatca
1801 ctgaagcttt ttagtgcatt agtggggagg agggtgggga gacataacca ctgcttccat
1861 ttaatggggt gcacctgtcc aataggcgta gctatccgga cagagcacgt ttgcagaagg
1921 gggactcttc ttccaggtag ctgaaagggg aagacctgac gtactctggt taggttagga
1981 cttgccctcg tggtggaaac ttttcttaaa aagttataac caacttttct attaaaagtg
2041 ggaattagga gagaaggtag gggttgggaa tcagagagaa tggctttggt ctcttgcttg
2101 tgggactagc ctggcttggg actaaatgcc ctgctctgaa cacgaagctt agtataaact
2161 gatggatatc cctaccttga aagaagaaaa ggttcttact gcttggtcct tgatttatca
2221 cacaaagcag aatagtattt ttatatttaa atgtaaagac aaaaaactat atgtatggtt
2281 ttgtggatta tgtgtgtttt gctaaaggaa aaaaccatcc aggtcacggg gcaccaaatt
2341 tgagacaaat agtcggatta gaaataaagc atctcatttt gagtagagag caagggaagt
2401 ggttcttaga tggtgatctg ggattaggcc ctcaagaccc ttttgggttt ctgccctgcc
2461 caccctctgg agaaggtggg cactggatta gttaacagac gacacgttac tagcagtcac
2521 ttgatctccg tggctttggt ttaaaagaca cacttgtcca cataggttta gagataagag
2581 ttggctgttc aacttgagca tgttactgac agagggggta ttggggttat tttctggtag
2641 gaatagcatg tcactaaagc aggccttttg atattaaatt ttttaaaaag caaaattata
2701 gaagtttaga ttttaatcaa atttgtaggg tttctaggta atttttacag aattgcttgt
2761 ttgcttcaac tgtctcctac ctctgctctt ggaggagatg gggacagggc tggagtcaaa
2821 acacttgtaa ttttgtatct tgatgtcttt gttaagactg ctgaagaatt attttttttc
2881 ttttataata aggaataaac cccaccttta ttccttcatt tcatctacca ttttctggtt
2941 cttgtgttgg ctgtggcagg ccagctgtgg ttttcttttg ccatgacaac ttctaattgc
3001 catgtacagt atgttcaaag tcaaataact cctcattgta aacaaactgt gtaactgccc
3061 aaagcagcac ttataaatca gcctaacata aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3121 aaaaaaaaaa aaaaaaa
//