LOCUS BC018723 2496 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens carbohydrate (N-acetylgalactosamine 4-0)
sulfotransferase 8, mRNA (cDNA clone MGC:31814 IMAGE:4137487),
complete cds.
ACCESSION BC018723
VERSION BC018723.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2496)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2496)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 40 Row: c Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361615.
FEATURES Location/Qualifiers
source 1..2496
/db_xref="H-InvDB:HIT000038422"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:31814 IMAGE:4137487"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2496
/gene="CHST8"
/db_xref="GeneID:64377"
/db_xref="HGNC:HGNC:15993"
/db_xref="MIM:610190"
CDS 759..2033
/gene="CHST8"
/codon_start=1
/product="carbohydrate (N-acetylgalactosamine 4-0)
sulfotransferase 8"
/protein_id="AAH18723.1"
/db_xref="GeneID:64377"
/db_xref="HGNC:HGNC:15993"
/db_xref="MIM:610190"
/translation="MTLRPGTMRLACMFSSILLFGAAGLLLFISLQDPTELAPQQVPG
IKFNIRPRQPHHDLPPGGSQDGDLKEPTERVTRDLSSGAPRGRNLPAPDQPQPPLQRG
TRLRLRQRRRRLLIKKMPAAATIPANSSDAPFIRPGPGTLDGRWVSLHRSQQERKRVM
QEACAKYRASSSRRAVTPRHVSRIFVEDRHRVLYCEVPKAGCSNWKRVLMVLAGLASS
TADIQHNTVHYGSALKRLDTFDRQGILHRLSTYTKMLFVREPFERLVSAFRDKFEHPN
SYYHPVFGKAILARYRANASREALRTGSGVRFPEFVQYLLDVHRPVGMDIHWDHVSRL
CSPCLIDYDFVGKFESMEDDANFFLSLIRAPRNLTFPRFKDRHSQEARTTARIAHQYF
AQLSALQRQRTYDFYYMDYLMFNYSKPFADLY"
BASE COUNT 483 a 830 c 759 g 424 t
ORIGIN
1 gggactcgtc ccagagtttg ctgccgccgc cgccgctgcc attagagcgg ttttaattag
61 ctgcaggatt tttaatgagc tgaaatcaag aagagcccca tctcagaagt aagcgagagc
121 cgcgcgcagg gaagaactag tctgacaaaa cagaaggagg tggtgctctc gggggactgg
181 gctgtaggct cccaccgatc gatcggcgac gagcagatga gttcccgagg ccaggaggct
241 gaggagggaa gccagggctc agagaccagc gcccggtgca gagtccccgc tgctgccacc
301 gcggcagaag gcacggtgcc ggattcaagg tgacggcagt gcctggctcc attctcctca
361 agttgccata gcagcctctc tcccaacaag cagcttctcc gggagccctc agccgggagg
421 gacgtcattg ttctctcctg cccgtgtggt gcaaggagac ccaccggaca gagaactttc
481 ccgagcccgg ggtccgctga ccacgtgggt gcacccatgc tgcctggagc caggcacagg
541 gacaggtccc cacggccaca gggaatcctc tgggcagctg aggggagcgt ccaggcccag
601 aagcagctgc agccaagcgt gtccttggag ccgtccatgc gtctgtccgc ctgctgccgg
661 tcgccactgg agatctcggc ctgatggacg cctggtgtgg acgatgaggg aagaacgtgc
721 cccccacacc caagaggtga cccctgagcc agccccggat gaccctgcga cctggaacaa
781 tgcggctggc ctgcatgttc tcttccatcc tgctgttcgg agctgcaggc ctcctcctct
841 tcatcagcct gcaggaccct acggagctcg ccccccagca ggtgccagga ataaagttca
901 acatcaggcc aaggcagccc caccacgacc tcccaccagg cggctcccag gatggtgact
961 tgaaggaacc cacagagagg gtcactcggg acttatccag tggggccccg aggggccgca
1021 acctgccagc gcctgaccag cctcaacccc cgctgcagag gggaacccgt ctgcggctcc
1081 gccagcgccg tcgccgtctg ctcatcaaga aaatgccagc tgcggcgacc atcccggcca
1141 acagctcgga cgcgcccttc atccggccgg gacccgggac gctggatggc cgctgggtca
1201 gcctgcaccg gagccagcag gagcgcaagc gggtgatgca ggaggcctgc gccaagtacc
1261 gggcgagcag cagccgccgg gccgtcacgc cccgccacgt gtcccgtatc ttcgtggagg
1321 accgccaccg cgtgctctac tgcgaggtgc ccaaggccgg ctgctccaat tggaagcggg
1381 tgctcatggt gctggccggc ctggcctcgt ccactgccga catccagcac aacaccgtcc
1441 actatggcag cgctctcaag cgcctggaca ccttcgaccg ccagggtatc ttgcaccgtc
1501 tcagcaccta caccaagatg ctctttgtcc gcgagccctt cgagaggctg gtgtccgcct
1561 tccgcgacaa gtttgagcac cccaacagct actatcaccc ggtcttcggc aaggccatcc
1621 tggcccggta ccgcgccaat gcctctcggg aggccctgcg gaccggctct ggggtgcgtt
1681 ttcccgagtt cgtccagtac ctgctggacg tgcaccggcc cgtggggatg gacattcact
1741 gggaccatgt cagccggctc tgcagcccct gcctcatcga ctacgatttc gtaggcaagt
1801 tcgagagcat ggaggacgat gccaacttct tcctgagcct catccgcgcg ccgcggaacc
1861 tgaccttccc ccggttcaag gaccggcact cgcaggaggc gcggaccaca gcgaggatcg
1921 cccaccagta cttcgcccaa ctctcggccc tgcaaaggca gcgcacctac gacttctact
1981 acatggatta cctgatgttc aactattcca agccctttgc agatctgtac tgaggggcgc
2041 cgcagctggc cggggccgcc ctgccccggt cactcacctg tgctcccggg catcctcctg
2101 tccctggctc ctcatcctgg gagcaacagg gctctgagga cgtgaggagc catcgctgtg
2161 ggaggcagca ggccccgggt ggggggcaga ggcgcccagc cttggatggg gaccccagcc
2221 cctggcctgt acctgtttcc tcattccttg gctgagggag aggctgagaa ctgggcagac
2281 acccctggag ctcagccgac agttttgatg agcagggaag tctgaggccc agaggacggg
2341 gggcccagcg gtaagggatg tcccgcactc ccttagccat tgccttggac caaaccacgt
2401 ggtttgcagc ttttctacga gccagggggg aggttccctt ggattaaggt tccaaataaa
2461 gcacatggtt tccagagcaa aaaaaaaaaa aaaaaa
//