LOCUS BC018645 1692 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens mitogen-activated protein kinase kinase 2, mRNA (cDNA
clone MGC:16530 IMAGE:4025987), complete cds.
ACCESSION BC018645
VERSION BC018645.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1692)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1692)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC018645.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 25 Row: n Column: 20.
FEATURES Location/Qualifiers
source 1..1692
/db_xref="H-InvDB:HIT000038381"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:16530 IMAGE:4025987"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1692
/gene="MAP2K2"
/gene_synonym="MAPKK2"
/gene_synonym="MEK2"
/gene_synonym="MKK2"
/db_xref="GeneID:5605"
/db_xref="HGNC:HGNC:6842"
/db_xref="MIM:601263"
CDS 197..1399
/gene="MAP2K2"
/gene_synonym="MAPKK2"
/gene_synonym="MEK2"
/gene_synonym="MKK2"
/codon_start=1
/product="mitogen-activated protein kinase kinase 2"
/protein_id="AAH18645.1"
/db_xref="GeneID:5605"
/db_xref="HGNC:HGNC:6842"
/db_xref="MIM:601263"
/translation="MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEEL
ELDEQQKKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMARKL
IHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLK
EAKRIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQ
LIDSMANSFVGTRSYMAPERLQGTHYSVQSDIWSMGLSLVELAVGRYPIPPPDAKELE
AIFGRPVVDGEEGEPHSISPRPRPPGRPVSGHGMDSRPAMAIFELLDYIVNEPPPKLP
NGVFTPDFQEFVNKCLIKNPAERADLKMLTNHTFIKRSEVEEVDFAGWLCKTLRLNQP
GTPTRTAV"
BASE COUNT 340 a 573 c 519 g 260 t
ORIGIN
1 gcggctcgct cgcctcagcc ccagcgcccc tcggctaccc tcggcccagg cccgcagcgc
61 cgcccgccct cggccgcccc gacgccggcc tgggccgcgg ccgcagcccc gggctcgcgt
121 aggcgccgac cgctcccggc ccgcccccta tgggccccgg ctagaggcgc cgccgccgcc
181 ggcccgcgga gccccgatgc tggcccggag gaagccggtg ctgccggcgc tcaccatcaa
241 ccctaccatc gccgagggcc catcccctac cagcgagggc gcctccgagg caaacctggt
301 ggacctgcag aagaagctgg aggagctgga acttgacgag cagcagaaga agcggctgga
361 agcctttctc acccagaaag ccaaggtcgg cgaactcaaa gacgatgact tcgaaaggat
421 ctcagagctg ggcgcgggca acggcggggt ggtcaccaaa gtccagcaca gaccctcggg
481 cctcatcatg gccaggaagc tgatccacct tgagatcaag ccggccatcc ggaaccagat
541 catccgcgag ctgcaggtcc tgcacgaatg caactcgccg tacatcgtgg gcttctacgg
601 ggccttctac agtgacgggg agatcagcat ttgcatggaa cacatggatg gcggctccct
661 ggaccaggtg ctgaaagagg ccaagaggat tcccgaggag atcctgggga aagtcagcat
721 cgcggttctc cggggcttgg cgtacctccg agagaagcac cagatcatgc accgagatgt
781 gaagccctcc aacatcctcg tgaactctag aggggagatc aagctgtgtg acttcggggt
841 gagcggccag ctcatagact ccatggccaa ctccttcgtg ggcacgcgct cctacatggc
901 tccggagcgg ttgcagggca cacattactc ggtgcagtcg gacatctgga gcatgggcct
961 gtccctggtg gagctggccg tcggaaggta ccccatcccc ccgcccgacg ccaaagagct
1021 ggaggccatc tttggccggc ccgtggtcga cggggaagaa ggagagcctc acagcatctc
1081 gcctcggccg aggccccccg ggcgccccgt cagcggtcac gggatggata gccggcctgc
1141 catggccatc tttgaactcc tggactatat tgtgaacgag ccacctccta agctgcccaa
1201 cggtgtgttc acccccgact tccaggagtt tgtcaataaa tgcctcatca agaacccagc
1261 ggagcgggcg gacctgaaga tgctcacaaa ccacaccttc atcaagcggt ccgaggtgga
1321 agaagtggat tttgccggct ggttgtgtaa aaccctgcgg ctgaaccagc ccggcacacc
1381 cacgcgcacc gccgtgtgac agtggccggg ctccctgcgt cccgctggtg acctgcccac
1441 cgtccctgtc catgccccgc ccttccagct gaggacaggc tggcgcctcc acccaccctc
1501 ctgcctcacc cctgcggaga gcaccgtggc ggggcgacag cgcatgcagg aacgggggtc
1561 tcctctcctg cccgtcctgg ccggggtgcc tctggggacg ggcgacgctg ctgtgtgtgg
1621 tctcagaggc tctgcttcct taggttacaa aacaaaacag ggagagaaaa agcaaaaaaa
1681 aaaaaaaaaa aa
//