LOCUS BC018445 2271 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens lactation elevated 1, mRNA (cDNA clone MGC:9915
IMAGE:3871205), complete cds.
ACCESSION BC018445
VERSION BC018445.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2271)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2271)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 13 Row: k Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 62241031.
FEATURES Location/Qualifiers
source 1..2271
/db_xref="H-InvDB:HIT000038347"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9915 IMAGE:3871205"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2271
/gene="LACE1"
/db_xref="GeneID:246269"
/db_xref="HGNC:HGNC:16411"
CDS 187..1632
/gene="LACE1"
/codon_start=1
/product="lactation elevated 1"
/protein_id="AAH18445.2"
/db_xref="GeneID:246269"
/db_xref="HGNC:HGNC:16411"
/translation="MAASWSLLVTLRPLAQSPLRGRCVGCGAWAAALAPLATAPGKPF
WKAYTVQTSESMTPTATSETYLKALAVCHGPLDHYDFLIKAHELKDDEHQRRVIQCLQ
KLHEDLKGYNIEAEGLFSKLFSRSKPPRGLYVYGDVGTGKTMVMDMFYAYVEMKRKKR
VHFHGFMLDVHKRIHRLKQSLPKRKPGFMAKSYDPIAPIAEEISEEACLLCFDEFQVT
DIADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQ
LDSGIDYRKRELPAAGKLYYLTSEADVEAVMDKLFDELAQKQNDLTRPRILKVQGREL
RLNKACGTVADCTFEELCERPLGASDYLELSKNFDTIFLRNIPQFTLANRTQGRRFIT
LIDNFYDLKVRIICSASTPISSLFLHQHHDSELEQSRILMDDLGLSQDSAEGLSMFTG
EEEIFAFQRTISRLTEMQTEQYWNEGDRTKK"
BASE COUNT 672 a 462 c 508 g 629 t
ORIGIN
1 cggaaggacg ggagggcacg ggagtgcagc ccgcccatgt ggctactgga ggtcacgttc
61 cctaactgat cccttggttc tctcgggtgg agccttcagc gtgcacggcg gggtttgact
121 ttgccaccgt ctctcttctg ggttccaata aagttttcct cttcctctcc tcgtacggag
181 ttcaagatgg cggcctcctg gtcgctcttg gttaccctgc gccccttagc acagagcccg
241 ctgagaggga gatgtgttgg gtgcggggcc tgggccgccg ctctcgctcc tctggccacc
301 gcccctggga agcccttttg gaaagcctat acggttcaga catccgagag catgacccca
361 actgccactt cagagactta tttgaaagct ttggccgttt gccatggacc tctggaccac
421 tatgattttc tgatcaaagc tcatgagcta aaggatgatg aacatcaaag aagagtcata
481 cagtgtttgc agaaattaca cgaggacctt aaaggataca atatagaggc agaaggcctt
541 ttttcaaagc ttttttcaag gagcaaacct ccaaggggcc tgtatgttta tggagatgtt
601 ggtacaggaa aaacaatggt gatggacatg ttttatgctt atgtggaaat gaagaggaaa
661 aaacgggttc attttcatgg tttcatgcta gatgtgcaca aaagaataca tcgccttaaa
721 cagagtttgc caaaaaggaa accaggattc atggctaaat catatgaccc aatagctccc
781 atagccgaag aaatcagcga agaagcatgt ctcctatgtt ttgatgaatt tcaggtcact
841 gacattgctg atgccatgat tctgaaacag ctttttgaaa atctgttcaa aaacggggtc
901 gtcgttgtgg caacatccaa caggccaccg gaagatctct ataaaaatgg actccaaaga
961 gctaactttg taccattcat agcagtcttg aaggaatatt gtaatacagt ccagctagat
1021 tctgggatag attaccggaa aagggaactt cctgctgcag gaaaactcta ctacctcaca
1081 agtgaagctg atgtggaggc tgtcatggat aagttgtttg atgagctggc tcagaaacaa
1141 aatgatttaa ctagaccaag gattctaaaa gtgcaaggca gagagctgcg cctgaataaa
1201 gcctgtggaa ccgttgccga ctgcacattt gaagagctgt gtgagagacc acttggagcc
1261 agtgactatt tggaactatc aaagaatttt gatacaatat ttttacgaaa cattccgcaa
1321 tttactctgg caaacaggac tcaaggtcga agattcataa ctctcatcga taacttttat
1381 gatctcaagg tgcgtataat ttgctctgcg tcgactccta tatcaagctt atttttgcat
1441 caacatcatg acagtgagtt ggagcaaagc agaatactga tggatgattt ggggctgagc
1501 caggattcag cagaaggact ctccatgttt accggagaag aggaaatctt tgcatttcag
1561 cgcacaattt cccgactcac ggaaatgcag actgaacagt actggaatga aggagacaga
1621 accaagaagt aactgccact tttgcataaa taaaactcta gacaaatggt taacgcaggc
1681 agaactcctt attgtgggac ttgaaggaat cattttctca tcattaatta tgcactttgt
1741 cctctggacc attttaatct aaaattgctc tcaaggatct agtggattag taagttaaac
1801 acttaacatt ttttaggtct gctttttctt atttggcctt atttctgcac catgaaatta
1861 aaatcatctc attcctgaag atgaatgtag gaccagacat tttagcttca cattaaacca
1921 cctgaatgaa ccttgaatgc acaagtaaat gcaagacgtc tggatacctt caagctttgg
1981 ctgcatggtg gtggcatgtt agaaatacat cttttataat gggggaagaa tgccctatgt
2041 ttcaattcaa aacaaagaca gttttccaat ctgatataaa ctaaaaggga taatatttta
2101 gaatatgggc ttatatgtgg tttttttttt ttggttatga attacagtaa atctcccagg
2161 ccttcagatt tctacataat ggaagaatat gtttttaacc ctgagatcaa gtttagtagg
2221 caggacttga tctcaagtgg tcacaaagtt ttctaaaaaa aaaaaaaaaa a
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