LOCUS BC018295 2255 bp mRNA linear HUM 04-JUN-2004
DEFINITION Homo sapiens phosphofructokinase, liver, mRNA (cDNA clone
IMAGE:3458877), partial cds.
ACCESSION BC018295
VERSION BC018295.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2255)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2255)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (03-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 19 Row: b Column: 13.
FEATURES Location/Qualifiers
source 1..2255
/db_xref="H-InvDB:HIT000038326"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3458877"
/tissue_type="Cervix, carcinoma"
/clone_lib="NIH_MGC_12"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2255
/gene="PFKL"
/db_xref="GeneID:5211"
/db_xref="MIM:171860"
CDS <1..1709
/gene="PFKL"
/codon_start=3
/product="PFKL protein"
/protein_id="AAH18295.2"
/db_xref="GeneID:5211"
/db_xref="MIM:171860"
/translation="GYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSR
LNIIIIAEGAIDRNGKPISSSYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSS
KMGMEAVMALLEATPDTPACVVTLSGNQSVRLPLMECVQMTKEVQKAMDDKRFDEATQ
LRGGSFENNWNIYKLLAHQKPPKEKSNFSLAILNVGAPAAGMNAAVRSAVRTGISHGH
TVYVVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKGQLESIVENIRIYG
IHALLVVGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVN
AAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDL
KVNVEHMTEKMKTDIQRGLVLRNEKCHDYYTTEFLYNLYSSEGKGVFDCRTNVLGHLQ
QGGAPTPFDRNYGTKLGVKAMLWLSEKLREVYRKGRVFANAPDSACVIGLKKKAVAFS
PVTELKKDTDFEHRMPREQWWLSLRLMLKMLAQYRISMAAYVSGELEHVTRRTLSMDK
GF"
BASE COUNT 446 a 675 c 721 g 413 t
ORIGIN
1 gcgggtacct ggcgctggta tctgcactgg cctcaggggc cgactggctg ttcatccccg
61 aggctccacc cgaggacggc tgggagaact tcatgtgtga gaggctgggt gagactcgga
121 gccgtgggtc ccgactgaac atcatcatca tcgctgaggg tgccattgac cgcaacggga
181 agcccatctc gtccagctac gtgaaggacc tggtggttca gaggctgggc ttcgacaccc
241 gtgtaactgt gctgggccac gtgcagcggg gagggacgcc ctctgccttc gaccggatcc
301 tgagcagcaa gatgggcatg gaggcggtga tggcgctgct ggaagccacg cctgacacgc
361 cggcctgcgt ggtcaccctc tcggggaacc agtcagtgcg gctgcccctc atggagtgcg
421 tgcagatgac caaggaagtg cagaaagcca tggatgacaa gaggtttgac gaggccaccc
481 agctccgtgg tgggagcttc gagaacaact ggaacattta caagctcctc gcccaccaga
541 agccccccaa ggagaagtct aacttctccc tggccatcct gaatgtgggg gccccggcgg
601 ctggcatgaa tgcggccgtg cgctcggcgg tgcggaccgg catctcccat ggacacacag
661 tatacgtggt gcacgatggc ttcgaaggcc tagccaaggg tcaggtgcaa gaagtaggct
721 ggcacgacgt ggccggctgg ttggggcgtg gtggctccat gctggggacc aagaggaccc
781 tgcccaaggg ccagctggag tccattgtgg agaacatccg catctatggt attcacgccc
841 tgctggtggt cggtgggttt gaggcctatg aaggggtgct gcagctggtg gaggctcgcg
901 ggcgctacga ggagctctgc atcgtcatgt gtgtcatccc agccaccatc agcaacaacg
961 tccctggcac cgacttcagc ctgggctccg acactgctgt aaatgccgcc atggagagct
1021 gtgaccgcat caaacagtct gcctcgggga ccaagcgccg tgtgttcatc gtggagacca
1081 tggggggtta ctgtggctac ctggccaccg tgactggcat tgctgtgggg gccgacgccg
1141 cctacgtctt cgaggaccct ttcaacatcc acgacttaaa ggtcaacgtg gagcacatga
1201 cggagaagat gaagacagac attcagaggg gcctggtgct gcggaacgag aagtgccatg
1261 actactacac cacggagttc ctgtacaacc tgtactcatc agagggcaag ggcgtcttcg
1321 actgcaggac caatgtcctg ggccacctgc agcagggtgg cgctccaacc ccctttgacc
1381 ggaactatgg gaccaagctg ggggtgaagg ccatgctgtg gttgtcggag aagctgcgcg
1441 aggtttaccg caagggacgg gtgttcgcca atgccccaga ctcggcctgc gtgatcggcc
1501 tgaagaagaa ggcggtggcc ttcagccccg tcactgagct caagaaagac actgatttcg
1561 agcaccgcat gccacgggag cagtggtggc tgagcctgcg gctcatgctg aagatgctgg
1621 cacaataccg catcagtatg gccgcctacg tgtcagggga gctggagcac gtgacccgcc
1681 gcaccctgag catggacaag ggcttctgag gccagccatg cccacgcccc tccccagccc
1741 ccacccatgc cagcgcagcg ccagggctca gatggggcct gggctgttgt gtctggagcc
1801 tgcaggcagg tgggggctgc gtccctgctc agcccatccc ctgcctctat ccctggccac
1861 ctgccaggcc tccctccggc tggtgtcttg agaccagcct gccaggccct ccagcaggag
1921 gacagagtgc cctggggcat ccaccttcct gcccagggga cgtggcgctg tcggtgtttg
1981 gaggctgctg ccccctggct ttggcgcccc atgggccctc agcgtctccc catgctgggc
2041 tcactacatg ggccagccct tgctctacct ggccggtagg ctgctggcgc ctaggttgtg
2101 ttgagagggg gatgcccctg gccctgcctc actgtgacct gctcctgccc acgtgcagca
2161 cctgtcacct tttctagaaa taaaatcacc ctgactgtgg ggtgcatcgg tctccggaga
2221 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa
//