LOCUS BC017843 1411 bp mRNA linear HUM 30-JAN-2008
DEFINITION Homo sapiens cholesterol 25-hydroxylase, mRNA (cDNA clone MGC:22529
IMAGE:4619357), complete cds.
ACCESSION BC017843
VERSION BC017843.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1411)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1411)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 36 Row: h Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31542304.
FEATURES Location/Qualifiers
source 1..1411
/db_xref="H-InvDB:HIT000038162"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:22529 IMAGE:4619357"
/tissue_type="Lung"
/clone_lib="NIH_MGC_77"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1411
/gene="CH25H"
/gene_synonym="C25H"
/db_xref="GeneID:9023"
/db_xref="HGNC:HGNC:1907"
/db_xref="MIM:604551"
CDS 23..841
/gene="CH25H"
/gene_synonym="C25H"
/codon_start=1
/product="cholesterol 25-hydroxylase"
/protein_id="AAH17843.1"
/db_xref="GeneID:9023"
/db_xref="HGNC:HGNC:1907"
/db_xref="MIM:604551"
/translation="MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFS
ITTYVGFCLPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPV
TLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVH
HQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGY
NFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVPAR"
BASE COUNT 337 a 384 c 299 g 391 t
ORIGIN
1 agctgccttg cacagcctcg caatgagctg ccacaactgc tccgaccccc aggtcctttg
61 cagctccggg cagctgttcc tgcagcccct ctgggaccac ctgaggagct gggaggccct
121 cctacagtcg cccttcttcc cggtcatctt ctccatcacc acatacgtgg gcttttgcct
181 gcccttcgtg gtcctggata tcctgtgctc ctgggtgccc gccctgcggc gctacaagat
241 ccaccctgac ttctcgccat ccgcgcagca gctgctacct tgcctggggc agaccctcta
301 ccagcatgtg atgtttgtgt tccccgtgac gctgctgcat tgggcccgca gcccggccct
361 cctgccccac gaagctcccg agctgctcct gctgctgcac cacatcctgt tctgcctgct
421 actcttcgac atggagttct tcgtgtggca cctgctgcac cacaaggtgc cctggctgta
481 ccgcaccttc cacaaggtgc accaccagaa ctcgtcctcg ttcgcgctgg caacgcagta
541 tatgagcgtc tgggaactgt tttctttggg cttcttcgac atgatgaacg tcacactgct
601 cgggtgccac ccgctcacca ccctgacctt ccacgtggtc aacatctggc tttccgtgga
661 ggaccactcc ggctacaact tcccttggtc cactcacaga ctggtgccct tcgggtggta
721 cgggggtgtg gtgcaccacg acctgcatca ctctcacttt aactgcaact tcgctccgta
781 ctttacacac tgggacaaaa tactgggaac gctgcggact gcatctgtcc cagcgcggtg
841 atgtggctgc ggtgggtgcc cctaagactc gggactgctg tgcctttcac acttgaatga
901 agagaaacac ctgagctata tattttttta aagcaactaa cttattgctt tatgtttatc
961 tatgaaaacc atagataaaa tctgatgcat ttttgtaatc tgacaaagta atttacatac
1021 tgtttgtgta tcaatacaat tttgtgttct tggtattctt agtctagctc acctcaatag
1081 ccttgaatcc tgcatatgaa ttagacattc atcactggca tatttagaat atctctaaaa
1141 ggacttgttt gtagaataag gaattttcta tgtttcaaag tgttctaaaa cctggctaaa
1201 agaatgtatt tttgtggatg gtgttgactt ctgactctaa aagcaatcaa acatgtttct
1261 gctggacagt gaccaagaat tatagtacct tcttatattt ttttatagaa ctgtatattt
1321 attttgaaag aaatgttatt cgtgctttaa aaaggaaaaa aaaccatgaa tcaaataaaa
1381 gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
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