LOCUS BC015743 3309 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens mannosidase, beta A, lysosomal, mRNA (cDNA clone
MGC:23037 IMAGE:4905535), complete cds.
ACCESSION BC015743
VERSION BC015743.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3309)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3309)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: o Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 24797157.
FEATURES Location/Qualifiers
source 1..3309
/db_xref="H-InvDB:HIT000037291"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23037 IMAGE:4905535"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3309
/gene="MANBA"
/gene_synonym="MANB1"
/db_xref="GeneID:4126"
/db_xref="HGNC:HGNC:6831"
/db_xref="MIM:609489"
CDS 91..2730
/gene="MANBA"
/gene_synonym="MANB1"
/codon_start=1
/product="mannosidase, beta A, lysosomal"
/protein_id="AAH15743.1"
/db_xref="GeneID:4126"
/db_xref="HGNC:HGNC:6831"
/db_xref="MIM:609489"
/translation="MRLHLLLLLALCGAGTTAAELSYSLRGNWSICNGNGSLELPGAV
PGCVHSALFQQGLIQDSYYRFNDLNYRWVSLDNWTYSKEFKIPFEISKWQKVNLILEG
VDTVSKILFNEVTIGETDNMFNRYSFDITNVVRDVNSIELRFQSAVLYAAQQSKAHTR
YQVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNICHLNYF
TFSPIYDKSAQEWNLEIESTFDVVSSKPVGGQVIVAIPKLQTQQTYSIELQPGKRIVE
LFVNISKNITVETWWPHGHGNQTGYNMTVLFELDGGLNIEKSAKVYFRTVELIEEPIK
GSPGLSFYFKINGFPIFLKGSNWIPADSFQDRVTSELLRLLLQSVVDANMNTLRVWGG
GIYEQDEFYELCDELGIMVWQDFMFACALYPTDQGFLDSVTAEVAYQIKRLKSHPSII
IWSGNNENEEALMMNWYHISFTDRPIYIKDYVTLYVKNIRELVLAGDKSRPFITSSPT
NGAETVAEAWVSQNPNSNYFGDVHFYDYISDCWNWKVFPKARFASEYGYQSWPSFSTL
EKVSSTEDWSFNSKFSLHRQHHEGGNKQMLYQAGLHFKLPQSTDPLRTFKDTIYLTQV
MQAQCVKTETEFYRRSRSEIVDQQGHTMGALYWQLNDIWQAPSWASLEYGGKWKMLHY
FAQNFFAPLLPVGFENENMFYIYGVSDLHSDYSMTLSVRVHTWSSLEPVCSRVTERFV
MKGGEAVCLYEEPVSELLRRCGNCTRESCVVSFYLSADHELLSPTNYHFLSSPKEAVG
LCKAQITAIISQQGDIFVFDLETSAVAPFVWLDVGSIPGRFSDNGFLMTEKTRTILFY
PWEPTSKNELEQSFHVTSLTDIY"
BASE COUNT 907 a 689 c 800 g 913 t
ORIGIN
1 ctacttccga gggggtgagt ggcttcaccg ccggtccctt gcagcgctgc ctttcgatct
61 ctccacatct cggtggcgcg ggatctcaag atgcgcctcc acctgctcct gctgctcgcg
121 ctgtgcggtg caggcaccac cgccgcggag ctcagttaca gcttgcgtgg caactggagc
181 atctgcaatg ggaacggctc gctggagctg cccggggcgg tccctggctg cgtgcacagc
241 gccttgttcc agcagggcct gatccaggat tcttactaca gatttaatga ccttaactac
301 agatgggtct ctttggataa ctggacctat agcaaagaat ttaaaatccc ctttgaaatt
361 agcaaatggc aaaaagtaaa tttgattctt gagggagtgg atacggtttc aaaaatcctg
421 ttcaatgaag tcactattgg ggaaacagac aatatgttca atagatatag ctttgatatt
481 accaacgtgg tcagggacgt gaactccatt gagctgcgtt tccagtcagc ggtgttgtat
541 gcagcacagc agagcaaagc tcacactcgc taccaggttc ccccagactg ccctccactt
601 gtgcagaagg gtgaatgcca tgtcaacttt gttcggaagg agcaatgttc ctttagttgg
661 gactgggggc cttcctttcc tacccaggga atctggaaag atgttagaat tgaagcctat
721 aatatttgtc acctgaacta cttcacattt tccccaatat atgataagag tgcccaggag
781 tggaatctgg aaatagagtc tacatttgat gttgtcagct caaagccagt tggtggtcaa
841 gtgatcgtag ccatccctaa gttgcaaaca caacagacat acagcattga acttcaacct
901 gggaaaagga ttgttgagct atttgtgaac attagcaaga atattactgt agaaacttgg
961 tggcctcatg gacatggaaa ccagactggg tacaacatga ctgttctttt tgaactggat
1021 ggaggcttaa atattgaaaa atcagctaag gtttatttta ggacagtgga acttatagaa
1081 gagcctataa aagggtctcc tggtttgagt ttctatttca aaattaatgg atttcccata
1141 tttctaaaag gctcaaactg gatcccagca gattcattcc aggaccgagt aacctctgag
1201 ttgttacggc tccttttaca gtctgttgtg gatgctaata tgaatactct tcgggtttgg
1261 ggaggaggaa tttatgagca ggatgaattc tatgaactct gtgatgaact aggaataatg
1321 gtatggcagg attttatgtt tgcctgtgcc ctttatccaa ctgatcaggg cttcctggat
1381 tcagtgacag cagaagttgc ctaccagatc aagagactga aatctcatcc ttctatcatc
1441 atatggagtg gcaataatga aaatgaggag gcgctgatga tgaattggta tcatatcagt
1501 ttcactgacc ggccaatcta catcaaggac tatgtgacac tctatgtgaa aaacatcaga
1561 gagctcgtac tggcaggaga caagagtcgt ccttttatta cgtccagtcc tacaaatggg
1621 gctgaaactg ttgcagaagc ctgggtctct caaaacccta atagcaatta ttttggtgat
1681 gtacattttt atgactatat cagtgattgc tggaactgga aagttttccc aaaagctcga
1741 tttgcatctg aatatggata tcagtcctgg ccgtccttca gtacattaga aaaggtctcg
1801 tctacagagg actggtcttt caatagcaag ttttcacttc atcgacaaca tcacgaaggt
1861 ggtaacaaac aaatgcttta tcaggctgga cttcatttca aactccccca aagcacagat
1921 ccattacgca catttaaaga taccatctac cttactcagg tgatgcaggc ccagtgtgtc
1981 aaaacagaaa ctgaattcta ccgccgtagt cgcagcgaga tagtggatca gcaagggcac
2041 acgatggggg cactttattg gcagttgaat gacatctggc aagctccttc ctgggcttct
2101 cttgagtacg gaggaaagtg gaaaatgctt cattactttg ctcagaattt ctttgctcca
2161 ctgttgccag taggctttga gaatgaaaac atgttctata tctatggtgt gtcagatctt
2221 cactcggatt attcgatgac actcagtgtg agagtccata catggagctc cctggagccc
2281 gtgtgctctc gtgtgactga acgttttgtg atgaaaggag gagaggctgt ctgcctttat
2341 gaggagccag tgtctgaatt gctgaggaga tgtgggaatt gcacacggga aagctgtgtg
2401 gtttcctttt acctttcagc tgaccatgaa ctcctgagcc cgaccaacta ccacttcctg
2461 tcctcaccga aggaggccgt ggggctctgc aaggcgcaga tcactgccat catctctcag
2521 caaggtgaca tatttgtttt tgacctggag acctcagctg tcgctccctt tgtttggttg
2581 gatgtaggaa gcatcccagg gagatttagt gacaatggtt tcctcatgac tgagaagaca
2641 cgaactatat tattttaccc ttgggagccc accagcaaga atgagttgga gcaatctttt
2701 catgtgacct ccttaacaga tatttactga aggaatctag gttgtatttt cagtggacaa
2761 tgggaataaa gcatttctaa agcaccgact ggagaggaag gcaacagaga caaggagaga
2821 agccgagaga catgtctgcg tgctgccacg catctgagcg attgctctgt gaagagttgt
2881 acactgaaca ttttcagggg aggctgttta cccaggcaat gtcctcaaac aagcctgtgc
2941 cggggtgtcc tggaatctgt gccaggactg tgtttttagc ccttcacctc tcagctttag
3001 caggacatga accagttata acaagatggc cctgcagctg gttacaagaa tgtgacatgg
3061 caggatctat ggaaccaaat ggaaggtttt gaggtgatgt aggtctttca cagttagctt
3121 tggggaatac agaatactca aataaagtgc tttgttatta tttcagaggg aatggcgatt
3181 gaaatgttac aacagagatt tcttggtggt agctatttgg gtaaaggtat atggatattt
3241 ttctgtacat gtgaaattat ataaaaataa aagttatata aattacattg aaaaaaaaaa
3301 aaaaaaaaa
//