LOCUS BC015641 1780 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens enolase 1, (alpha), mRNA (cDNA clone MGC:23319
IMAGE:4643088), complete cds.
ACCESSION BC015641
VERSION BC015641.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1780)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1780)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (04-OCT-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC015641.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 33 Row: e Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1780
/db_xref="H-InvDB:HIT000037234"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23319 IMAGE:4643088"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1780
/gene="ENO1"
/gene_synonym="MBP-1"
/gene_synonym="NNE"
/gene_synonym="PPH"
/db_xref="GeneID:2023"
/db_xref="HGNC:HGNC:3350"
/db_xref="MIM:172430"
CDS 100..1404
/gene="ENO1"
/gene_synonym="MBP-1"
/gene_synonym="NNE"
/gene_synonym="PPH"
/codon_start=1
/product="enolase 1, (alpha)"
/protein_id="AAH15641.1"
/db_xref="GeneID:2023"
/db_xref="HGNC:HGNC:3350"
/db_xref="MIM:172430"
/translation="MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIY
EALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTEN
KSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAG
NKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNIL
ENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQL
ADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNE
KSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQ
IKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK"
BASE COUNT 428 a 474 c 485 g 393 t
ORIGIN
1 ggagcacgga gatctcgccg gctttacgtt cacctcggtg tctgcagcac cctccgcttc
61 ctctcctagg cgacgagacc cagtggctag aagttcacca tgtctattct caagatccat
121 gccagggaga tctttgactc tcgcgggaat cccactgttg aggttgatct cttcacctca
181 aaaggtctct tcagagctgc tgtgcccagt ggtgcttcaa ctggtatcta tgaggcccta
241 gagctccggg acaatgataa gactcgctat atggggaagg gtgtctcaaa ggctgttgag
301 cacatcaata aaactattgc gcctgccctg gttagcaaga aactgaacgt cacagaacaa
361 gagaagattg acaaactgat gatcgagatg gatggaacag aaaataaatc taagtttggt
421 gcgaacgcca ttctgggggt gtcccttgcc gtctgcaaag ctggtgccgt tgagaagggg
481 gtccccctgt accgccacat cgctgacttg gctggcaact ctgaagtcat cctgccagtc
541 ccggcgttca atgtcatcaa tggcggttct catgctggca acaagctggc catgcaggag
601 ttcatgatcc tcccagtcgg tgcagcaaac ttcagggaag ccatgcgcat tggagcagag
661 gtttaccaca acctgaagaa tgtcatcaag gagaaatatg ggaaagatgc caccaatgtg
721 ggggatgaag gcgggtttgc tcccaacatc ctggagaata aagaaggcct ggagctgctg
781 aagactgcta ttgggaaagc tggctacact gataaggtgg tcatcggcat ggacgtagcg
841 gcctccgagt tcttcaggtc tgggaagtat gacctggact tcaagtctcc cgatgacccc
901 agcaggtaca tctcgcctga ccagctggct gacctgtaca agtccttcat caaggactac
961 ccagtggtgt ctatcgaaga tccctttgac caggatgact ggggagcttg gcagaagttc
1021 acagccagtg caggaatcca ggtagtgggg gatgatctca cagtgaccaa cccaaagagg
1081 atcgccaagg ccgtgaacga gaagtcctgc aactgcctcc tgctcaaagt caaccagatt
1141 ggctccgtga ccgagtctct tcaggcgtgc aagctggccc aggccaatgg ttggggcgtc
1201 atggtgtctc atcgttcggg ggagactgaa gataccttca tcgctgacct ggttgtgggg
1261 ctgtgcactg ggcagatcaa gactggtgcc ccttgccgat ctgagcgctt ggccaagtac
1321 aaccagctcc tcagaattga agaggagctg ggcagcaagg ctaagtttgc cggcaggaac
1381 ttcagaaacc ccttggccaa gtaagctgtg ggcaggcaag cccttcggtc acctgttggc
1441 tacacagacc cctcccctcg tgtcagctca ggcagctcga ggcccccgac caacacttgc
1501 aggggtccct gctagttagc gccccaccgc cgtggagttc gtaccgcttc cttagaactt
1561 ctacagaagc caagctccct ggagccctgt tggcagctct agctttgcag tcgtgtaatt
1621 ggcccaagtc attgtttttc tcgcctcact ttccaccaag tgtctagagt catgtgagcc
1681 tcgtgtcatc tccggggtgg ccacaggcta gatccccggt ggttttgtgc tcaaaataaa
1741 aagcctcagt gacccatgaa aaaaaaaaaa aaaaaaaaaa
//