LOCUS BC014982 2479 bp mRNA linear HUM 06-MAR-2007
DEFINITION Homo sapiens histidyl-tRNA synthetase 2, mitochondrial (putative),
mRNA (cDNA clone MGC:23192 IMAGE:4859849), complete cds.
ACCESSION BC014982
VERSION BC014982.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2479)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2479)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-OCT-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: f Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: Similarity but not
identity to protein.
FEATURES Location/Qualifiers
source 1..2479
/db_xref="H-InvDB:HIT000036942"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23192 IMAGE:4859849"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2479
/gene="HARS2"
/gene_synonym="HARSR"
/gene_synonym="HO3"
/db_xref="GeneID:23438"
/db_xref="HGNC:HGNC:4817"
/db_xref="MIM:600783"
CDS 188..1708
/gene="HARS2"
/gene_synonym="HARSR"
/gene_synonym="HO3"
/codon_start=1
/product="histidyl-tRNA synthetase 2, mitochondrial
(putative)"
/protein_id="AAH14982.1"
/db_xref="GeneID:23438"
/db_xref="HGNC:HGNC:4817"
/db_xref="MIM:600783"
/translation="MPLLGLLPRRAWASLLSQLLRPPCASCTGAVRCQSQVAEAVLTS
QLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKE
TLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRE
SPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIV
DGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHG
GVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTG
VIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFY
IVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLL
TQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES"
BASE COUNT 624 a 540 c 689 g 626 t
ORIGIN
1 ctggctacta agggaacttg ggaggatccc acctcagcct tcgtgactag tgaggtgcgc
61 aaacgcccga gttttccctg gtgcgcgggt tccgcctttg cagtgccctc cacccttcct
121 ggtgtctgac ccgcctcctt cccaggcctt ttgttcctgt cccggaaagc cggcgtcctg
181 ccgcgcgatg cccctgctcg gacttcttcc caggagggcc tgggcttcgc tgctcagcca
241 gctcctgcga ccgccctgcg cttcgtgcac cggggcggtc cgttgccaaa gccaggttgc
301 agaggcagtg ttaacatccc aactgaaagc acatcaagag aaaccaaatt ttattatcaa
361 gaccccaaag ggtaccaggg atcttagtcc tcagcatatg gttgtgaggg agaaaattct
421 tgatttggtt atcagctgct ttaaacgtca tggagcaaag gggatggaca ccccagcatt
481 tgagctgaag gaaaccctga ctgagaagta tggagaggac tctgggctca tgtatgatct
541 gaaggatcaa ggtggagagc tgttgtccct ccgctatgac cttactgttc cctttgctcg
601 ttatctggcc atgaataagg tgaagaagat gaaacgttat catgttggaa aggtgtggcg
661 gcgagagagc ccaaccatag tccaaggccg ttatagggag ttctgccagt gtgattttga
721 cattgctggt cagtttgacc ctatgatccc cgatgcagag tgtttgaaga tcatgtgtga
781 aatcctaagt ggattgcagt tgggagactt tctcattaag gtaaatgacc ggcggattgt
841 ggatgggatg tttgctgtct gtggtgttcc tgaaagcaag ttccgtgcca tctgctcctc
901 catagataaa ctagacaaga tggcttggaa agatgtgaga catgagatgg tggtgaagaa
961 aggcctggct cctgaggtgg ctgatcgaat tggggactat gtccagtgtc atggtggggt
1021 atccctagta gagcaaatgt ttcaggatcc cagactatcc cagaacaagc aggccctgga
1081 gggcctggga gacctaaagc tgctatttga atacctgact ttatttggaa ttgctgataa
1141 gatctccttt gacctcagcc tggctcgggg cctagactac tatacaggag tgatctatga
1201 agcagtgctg ctgcagaccc caactcaggc tggggaggag cccctgaatg tgggcagtgt
1261 ggctgctggt gggcgctatg atgggctggt gggcatgttt gaccccaagg gccacaaggt
1321 gccatgtgtg ggactcagca ttggggttga gcgaatcttc tacattgtgg agcagaggat
1381 gaagaccaaa ggtgagaagg tgcggactac agagactcaa gtgtttgtgg ccacaccaca
1441 gaagaacttt ctccaagaac ggttgaagct tattgcagag ctttgggatt ctggaatcaa
1501 ggcagagatg ctatacaaga acaaccccaa actattaacc cagctgcact attgtgagag
1561 cacaggcatt ccactggtgg tcattattgg tgagcaagaa ctgaaagaag gggtcatcaa
1621 gatccgttca gtggccagca gagaggaggt ggccattaaa cgggaaaatt ttgtggctga
1681 aattcagaag cgactgtctg agtcttgatc cttgcctgat tcccatctgc tgctctttgt
1741 agaaaaggtt tcctctagaa ctgaattcct ctggaattga gtgatggact tcacaacaac
1801 tagccaggaa gggtgacaag taccttctgc ctcctccatt cttcctgggt gcagaactgt
1861 agaagaccct gaggactcct gggctagtgt gagcagctat tctgcatggg tgcagatggc
1921 tggatgtgaa agagacatgc tctagctgca gaggcaaatt tgaagtgcca cggaacgttg
1981 tcaagaggta gtgagattgt tgctgtgagc aaggctctgg gagagtcacc tcaggctcag
2041 gattctgaac cattgagatc agaaaccaga tacttagcct tctactgtag ctacggaacc
2101 agattctggt gaatttgtcc acaatcagcc attggcctgt ctggggagtt tttggaagac
2161 agcaaagagg ggcaatggct gctttcagct ttgaggacag atgctatcct aagggcatgg
2221 cagtacccat gttgatttga catctctcta gcccatccat tgcttacagt agaagagtgg
2281 ggctgtatgc ttgataatct tgaacattga acttaataga tgacttgaca aggtagagat
2341 ctgatatttt gagattttgg agaccatttc ctgtgccaga atcactgctc tattccatac
2401 cgtgccatgg aggctgtttt agaagtggtt ggataacatg ttaaataaaa tattaagtgt
2461 aaaaaaaaaa aaaaaaaaa
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