LOCUS BC014942 2509 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ras homolog gene family, member T2, mRNA (cDNA clone
MGC:22917 IMAGE:4859240), complete cds.
ACCESSION BC014942
VERSION BC014942.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2509)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2509)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (01-OCT-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: f Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 20270302.
FEATURES Location/Qualifiers
source 1..2509
/db_xref="H-InvDB:HIT000036908"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:22917 IMAGE:4859240"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2509
/gene="RHOT2"
/gene_synonym="MIRO-2"
/gene_synonym="RASL"
/db_xref="GeneID:89941"
/db_xref="HGNC:HGNC:21169"
CDS 68..1924
/gene="RHOT2"
/gene_synonym="MIRO-2"
/gene_synonym="RASL"
/codon_start=1
/product="ras homolog gene family, member T2"
/protein_id="AAH14942.1"
/db_xref="GeneID:89941"
/db_xref="HGNC:HGNC:21169"
/translation="MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPPRAEEITIPA
DVTPEKVPTHIVDYSEAEQTDEELREEIHKANVVCVVYDVSEEATIEKIRTKWIPLVN
GGTTQGPRVPIILVGNKSDLRSGSSMEAVLPIMSQFPEIETCVECSAKNLRNISELFY
YAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDLDQALSDEELNAFQKSCFG
HPLAPQALEDVKTVVCRNVAGGVREDRLTLDGFLFLNTLFIQRGRHETTWTILRRFGY
SDALELTADYLSPLIHVPPGCSTELNHLGYQFVQRVFEKHDQDRDGALSPVELQSLFS
VFPAAPWGPELPRTVRTEAGRLPLHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQD
QAHAITVTREKRLDQEKGQTQRSVLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQP
PGYAIDTVQVNGQEKYLILCEVGTDGLLATSLDATCDVACLMFDGSDPKSFAHCASVY
KHHYMDGQTPCLFVSSKADLPEGVAVSGPSPAEFCRKHRLPAPVPFSCAGPAEPSTTI
FTQLATMAAFPHLVHAELHPSSFWLRGLLGVVGAAVAAVLSFSLYRVLVKSQ"
BASE COUNT 453 a 791 c 797 g 468 t
ORIGIN
1 gagcgaaagg cttgaggacc aggtcggggc cgggttccgg gtcggggagc ggctccgggc
61 ggcagctatg aggcgggacg tgcgcatcct gttactgggc gaggcccagg tggggaagac
121 gtcgctgatc ctgtccctgg tgggcgagga gttccccgag gaggtccctc cccgcgcgga
181 ggagatcacg atccccgcgg acgtcacccc ggagaaggtg cccacccaca tcgtggacta
241 ctcagaagcc gagcagacgg acgaggagct gcgggaggag atccacaagg caaacgtggt
301 gtgtgtggtg tatgacgtct ctgaggaggc caccattgag aagattcgaa ctaagtggat
361 cccactggtg aatgggggga ccacgcaggg gcccagggtg cccatcatcc tagtgggcaa
421 caagtcagac ctgcggtcgg ggagctccat ggaggccgtg ctccccatca tgagccagtt
481 tcccgagatt gagacctgcg tggagtgttc ggccaagaac ctgaggaaca tctcagagct
541 gttctactac gcccagaagg ccgtcctgca tcccacagcc cccctctatg accctgaggc
601 caagcagttg aggcccgcgt gcgcccaggc gctgacgcgc atcttcaggc tctcagatca
661 ggacctggac caggcgctca gtgacgaaga gctcaacgct ttccagaaat cctgctttgg
721 gcaccccctg gccccgcagg ccctggagga cgtgaagacg gtggtgtgca ggaacgtggc
781 gggcggcgtg cgggaggacc ggctgaccct ggatggtttc ctcttcctga acacgctctt
841 catccagcgc ggccggcacg agaccacctg gaccatcctg cggcgcttcg gctacagcga
901 tgccctggag ctgactgcgg actatctctc ccctctgatc cacgtgcccc ccggctgcag
961 cacggagctc aaccaccttg gctaccagtt tgtgcagaga gtgtttgaga agcacgacca
1021 ggaccgcgac ggcgccctct cgcccgtgga gctgcaaagc cttttcagtg tgttcccagc
1081 agcgccctgg ggccccgagc tcccacgcac agtccgcaca gaggccggcc ggttgcccct
1141 gcacggatac ctctgccagt ggaccctggt gacctacctg gacgtccgga gctgccttgg
1201 acacctaggc tacctgggct accccaccct ctgtgagcag gaccaggccc atgccatcac
1261 agtcactcgt gagaagaggc tggaccagga gaagggacag acgcagcgga gcgtcctcct
1321 gtgcaaggtg gtaggggccc gtggagtggg caagtctgcc ttcctgcagg cttttctcgg
1381 ccgcggcctg gggcaccagg acacgaggga gcagcctccc ggctacgcca tcgacacggt
1441 gcaggtcaat ggacaggaga agtacttgat cctctgtgag gtgggcacag atggtctgct
1501 ggccacatcg ctggacgcca cctgtgacgt tgcctgcttg atgtttgatg gcagtgaccc
1561 aaagtccttt gcacattgtg ccagcgtcta caagcaccat tacatggacg ggcagacccc
1621 ctgcctcttt gtctcctcca aggccgacct gcccgaaggt gtcgcggtgt ctggcccatc
1681 accggccgag ttttgccgca agcaccggct acccgctccc gtgccgttct cctgtgctgg
1741 cccagccgag cccagcacca ccatcttcac ccagctcgcc accatggccg ccttcccaca
1801 tttggtccac gcagagctgc atccctcttc cttctggctc cgggggctgc tgggggttgt
1861 cggggccgcc gtggccgcag tcctcagctt ctcactctac agggtcctgg tgaagagcca
1921 gtgaggcccc tggtacccaa gccccctccc ctgacctggg tgtgcctcgc tgctggggct
1981 ctgcaggggc agcacagctg gggtgcaggc caggctgcca ctccgggaac gcctttgcgc
2041 cgggactttt tgtttctgaa ggcagtcgat ctgcagcggg gccttatgct gccatgcact
2101 gccctggctc ctgccggacc cccagggtgg gccgtggcag gtggctgagc aggagctccc
2161 aagtgccggc caccgctgtc agggattgcc cacccctggg catcatgtgt gtggggccgg
2221 ggagcacagg tgtgggagct ggtgacccca gacccagaat tctcagggct ctacccccct
2281 ttcctggtcc taggtggcca gtgggtatga ggagggctgg aaggcagagc tttgggccaa
2341 aagcaggcgt tggggggtcc cccctcaagt ttggagccgt ttccgtggtt gtagcagagg
2401 accggaggtt gggttcctga ttaaacttca ctgtgtgttt tctatctcgg atcccagtct
2461 ctgaagacaa cttgctttga ttcaacctaa aaaaaaaaaa aaaaaaaaa
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