LOCUS BC014621 1959 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens asparagine synthetase, mRNA (cDNA clone MGC:4148
IMAGE:3010719), complete cds.
ACCESSION BC014621
VERSION BC014621.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1959)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1959)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (24-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC014621.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 10 Row: i Column: 22
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19718771.
FEATURES Location/Qualifiers
source 1..1959
/db_xref="H-InvDB:HIT000036817"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4148 IMAGE:3010719"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1959
/gene="ASNS"
/gene_synonym="TS11"
/db_xref="GeneID:440"
/db_xref="HGNC:HGNC:753"
/db_xref="MIM:108370"
CDS 128..1813
/gene="ASNS"
/gene_synonym="TS11"
/codon_start=1
/product="asparagine synthetase"
/protein_id="AAH14621.1"
/db_xref="GeneID:440"
/db_xref="HGNC:HGNC:753"
/db_xref="MIM:108370"
/translation="MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCC
FGFHRLAVVDPLFGMQPIRVKKYPYLWLCYNGEIYNHKKMQQHFEFEYQTKVDGEIIL
HLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSE
AKGLVTLKHSATPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDN
VEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKE
AQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETY
DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESER
LLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRE
TFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQKFPFN
TPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA"
BASE COUNT 565 a 395 c 448 g 551 t
ORIGIN
1 gccgcccgtc aagctgtcca catccctggc ctcagcccgc cacatcaccc tgacctgctt
61 acgcccagat tttcttcaat cacatctgaa taaatcactt gaagaaagct tatagcttca
121 ttgcaccatg tgtggcattt gggcgctgtt tggcagtgat gattgccttt ctgttcagtg
181 tctgagtgct atgaagattg cacacagagg tccagatgca ttccgttttg agaatgtcaa
241 tggatacacc aactgctgct ttggatttca ccggttggcg gtagttgacc cgctgtttgg
301 aatgcagcca attcgagtga agaaatatcc gtatttgtgg ctctgttaca atggtgaaat
361 ctacaaccat aagaagatgc aacagcattt tgaatttgaa taccagacca aagtggatgg
421 tgagataatc cttcatcttt atgacaaagg aggaattgag caaacaattt gtatgttgga
481 tggtgtgttt gcatttgttt tactggatac tgccaataag aaagtgttcc tgggtagaga
541 tacatatgga gtcagacctt tgtttaaagc aatgacagaa gatggatttt tggctgtatg
601 ttcagaagct aaaggtcttg ttacattgaa gcactccgcg actccctttt taaaagtgga
661 gccttttctt cctggacact atgaagtttt ggatttaaag ccaaatggca aagttgcatc
721 cgtggaaatg gttaaatatc atcactgtcg ggatgtaccc ctgcacgccc tctatgacaa
781 tgtggagaaa ctctttccag gttttgagat agaaactgtg aagaacaacc tcaggatcct
841 ttttaataat gctgtaaaga aacgtttgat gacagacaga aggattggct gccttttatc
901 agggggcttg gactccagct tggttgctgc cactctgttg aagcagctga aagaagccca
961 agtacagtat cctctccaga catttgcaat tggcatggaa gacagccccg atttactggc
1021 tgctagaaag gtggcagatc atattggaag tgaacattat gaagtccttt ttaactctga
1081 ggaaggcatt caggctctgg atgaagtcat attttccttg gaaacttatg acattacaac
1141 agttcgtgct tcagtaggta tgtatttaat ttccaagtat attcggaaga acacagatag
1201 cgtggtgatc ttctctggag aaggatcaga tgaacttacg cagggttaca tatattttca
1261 caaggctcct tctcctgaaa aagccgagga ggagagtgag aggcttctga gggaactcta
1321 tttgtttgat gttctccgcg cagatcgaac tactgctgcc catggtcttg aactgagagt
1381 cccatttcta gatcatcgat tttcttccta ttacttgtct ctgccaccag aaatgagaat
1441 tccaaagaat gggatagaaa aacatctcct gagagagacg tttgaggatt ccaatctgat
1501 acccaaagag attctctggc gaccaaaaga agccttcagt gatggaataa cttcagttaa
1561 gaattcctgg tttaagattt tacaggaata cgttgaacat caggttgatg atgcaatgat
1621 ggcaaatgca gcccagaaat ttcccttcaa tactcctaaa accaaagaag gatattacta
1681 ccgtcaagtc tttgaacgcc attacccagg ccgggctgac tggctgagcc attactggat
1741 gcccaagtgg atcaatgcca ctgacccttc tgcccgcacg ctgacccact acaagtcagc
1801 tgtcaaagct taggtggtct ttatgctgta atgtgaaagc aaatatttct tcgtgttgga
1861 tggggactgt gggtagatag gggaacaatg agagtcaact caggctaact tgggtgtgaa
1921 aaaaataaaa gtcctaaatc taaaaaaaaa aaaaaaaaa
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