LOCUS BC014479 1400 bp mRNA linear HUM 19-NOV-2003
DEFINITION Homo sapiens PX domain containing serine/threonine kinase, mRNA
(cDNA clone MGC:23156 IMAGE:4856139), complete cds.
ACCESSION BC014479
VERSION BC014479.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1400)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1400)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (17-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Louis Staudt
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield,
Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin,
Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo
Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven
Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline
Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott,
Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy,
George Yang, Scott Zuyderduyn, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: d Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 23618917.
FEATURES Location/Qualifiers
source 1..1400
/db_xref="H-InvDB:HIT000036728"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23156 IMAGE:4856139"
/tissue_type="Primary B-Cells from Tonsils"
/clone_lib="NIH_MGC_48"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1400
/gene="PXK"
/gene_synonym="FLJ20335"
/db_xref="GeneID:54899"
CDS 16..1368
/gene="PXK"
/gene_synonym="FLJ20335"
/codon_start=1
/product="PXK protein"
/protein_id="AAH14479.1"
/db_xref="GeneID:54899"
/translation="MAFMEKPPAGKVLLDDTVPLTAAIEASQSLQSHTEYIIRVQRGI
SVENSWQIVRRYSDFDLLNNSLQIAGLSLPLPPKKLIGNMDREFIAERQKGLQNYLNV
ITTNHILSNCELVKKFLDPNNYSANYTEIALQQVSMFFRSEPKWEVVEPLKDIGWRIR
KKYFLMKIKNQPKERLVLSWADLGPDKYLSDKDFQCLIKLLPSCLHPYIYRVTFATAN
ESSALLIRMFNEKGTLKDLIYKAKPKDPFLKKYCNPKKIQGLELQQIKTYGRQILEVL
KFLHDKGFPYGHLHASNVMLDGDTCRLLDLENSLLGLPSFYRSYFSQFRKINTLESVD
VHCFGHLLYEMTYGRPPDSVPVDSFPPAPSMAVVAVLESTLSCEACKNGMPTISRLLQ
MPLFSDVLLTTSEKPQFKIPTKLKEALRIAKECIEKRLIEEQKQIHQHRRLTRAQSHH
"
misc_feature 109..381
/gene="PXK"
/gene_synonym="FLJ20335"
/note="PX; Region: PX domain. PX domains bind to
phosphoinositides"
/db_xref="CDD:pfam00787"
misc_feature 607..1062
/gene="PXK"
/gene_synonym="FLJ20335"
/note="S_TKc; Region: Serine/Threonine protein kinases,
catalytic domain. Phosphotransferases of the serine or
threonine-specific kinase subfamily. The enzymatic
activity of these protein kinases is controlled by
phosphorylation of specific residues in the activation
segment of the catalytic domain, sometimes combined with
reversible conformational changes in the C-terminal
autoregulatory tail"
/db_xref="CDD:cd00180"
BASE COUNT 425 a 306 c 315 g 354 t
ORIGIN
1 gccgggcgtc ccgggatggc cttcatggag aagccgccag ccggcaaggt gctgctggac
61 gacacggtgc cgctgacagc agccatcgag gcgagccaga gcctgcagtc ccacacggaa
121 tatattattc gagtgcaaag aggaatttct gtggaaaaca gctggcagat tgttagaaga
181 tacagtgact ttgatttgct taacaacagc ttacagattg caggcctaag tctacctctt
241 cctcccaaaa aattgattgg taacatggat cgtgaattca tagctgaaag gcagaaaggt
301 cttcagaact atctcaacgt gatcacaaca aatcatatct tgtctaattg tgagctggtt
361 aagaagtttt tagatccaaa caactattcc gcaaactata ctgagattgc cttgcaacag
421 gtttccatgt tcttccgatc agaaccaaag tgggaggtgg tggaaccttt gaaagacata
481 ggttggagaa taaggaagaa atatttcttg atgaagatta aaaatcagcc aaaggaacgg
541 ctagtgttaa gctgggctga ccttggccca gacaagtatt tgtcagataa agattttcag
601 tgtctaatca aacttctgcc ttcttgtttg cacccttaca tctatcgggt tacctttgcc
661 acagctaatg aatcctcagc gttgctaatt aggatgttta acgaaaaggg aacattgaag
721 gatctgatct acaaggcaaa accaaaagac ccatttctaa agaagtactg caaccctaag
781 aagattcagg gcctggaact ccagcaaata aaaacatatg gacggcaaat attagaggta
841 ctgaagtttc ttcatgacaa gggattccct tatgggcatc ttcacgcctc caatgtgatg
901 ctcgatgggg acacttgtcg gctgctggac cttgagaatt ccttattggg cctgccttcc
961 ttctaccgat cttatttttc acaattcagg aaaatcaata cattggaaag tgtggatgtc
1021 cactgctttg gccacttact gtatgaaatg acttatggac gaccgccaga ctcggtgcct
1081 gtggactcct tccctcctgc cccgtccatg gctgtggtgg ccgtgttgga gtctacgctg
1141 tcttgtgaag cctgtaaaaa tggcatgcct accatctccc ggctcttaca gatgccatta
1201 ttcagcgatg ttttactaac cacttctgaa aaaccacagt ttaagatccc tacaaagtta
1261 aaagaggcat tgagaattgc caaagaatgt atagagaaga gactaattga ggaacagaaa
1321 cagattcacc agcatcgaag actgacaaga gctcagtccc accattgatc tgaggaggaa
1381 agaaaaaaaa aaaaaaaaaa
//