LOCUS BC014445 3517 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens EH-domain containing 2, mRNA (cDNA clone MGC:22994
IMAGE:4908085), complete cds.
ACCESSION BC014445
VERSION BC014445.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3517)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3517)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (17-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: p Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361461.
FEATURES Location/Qualifiers
source 1..3517
/db_xref="H-InvDB:HIT000036704"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:22994 IMAGE:4908085"
/tissue_type="Pancreas, epithelioid carcinoma"
/clone_lib="NIH_MGC_42"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3517
/gene="EHD2"
/db_xref="GeneID:30846"
/db_xref="HGNC:HGNC:3243"
/db_xref="MIM:605890"
CDS 162..1793
/gene="EHD2"
/codon_start=1
/product="EH-domain containing 2"
/protein_id="AAH14445.1"
/db_xref="GeneID:30846"
/db_xref="HGNC:HGNC:3243"
/db_xref="MIM:605890"
/translation="MFSWLKRGGARGQQPEAIRTVTSALKELYRTKLLPLEEHYRFGA
FHSPALEDADFDGKPMVLVAGQYSTGKTSFIQYLLEQEVPGSRVGPEPTTDCFVAVMH
GDTEGTVPGNALVVDPDKPFRKLNPFGNTFLNRFMCAQLPNQVLESISIIDTPGILSG
AKQRVSRGYDFPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGHEDKIRVVL
NKADMVETQQLMRVYGALMWALGKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQ
DLFRDIQGLPRHAALRKLNDLVKRARLVRVHAYIISYLKKEMPSVFGKENKKKQLILK
LPVIFAKIQLEHHISPGDFPDCQKMQELLMAHDFTKFHSLKPKLLEALDEMLTHDIAK
LMPLLRQEELESTEVGVQGGAFEGTHMGPFVERGPDEAMEDGEEGSDDEAEWVVTKDK
SKYDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIWKLSDVDRDGMLDDEEFAL
ASHLIEAKLEGHGLPANLPRRLVPPSKRRHKGSAE"
BASE COUNT 678 a 1184 c 956 g 699 t
ORIGIN
1 gacgggaccg cgagcacagg ccgctccgcg ggcgcttcgg atcctcgcgg gaccccaccc
61 tctcccagcc tgcccagccc gctgcagccg ccagcgcgcc ccgtcggcag ctctccatct
121 gcacgtctct ccgtgaaccc cgtgagcggt gtgcagccac catgttcagc tggctgaagc
181 ggggcggggc acggggccag cagcccgagg ccatccgcac ggtgacctcg gccctcaagg
241 agctgtaccg cacgaagctg ctgccgctgg aggagcacta ccgctttggg gccttccact
301 cgccggccct ggaggacgca gacttcgacg gcaagcccat ggtgctggtg gccggccagt
361 acagcacggg caagaccagc ttcatccagt acctgctgga gcaggaggtg cccggctccc
421 gcgtggggcc tgagcccacc accgactgct ttgtggccgt catgcacggg gacactgagg
481 gcaccgtgcc cggcaacgcc ctcgtcgtgg acccggacaa gcccttccgc aaactcaacc
541 ctttcggaaa caccttcctc aacaggttca tgtgtgccca gctccctaat caggtcctgg
601 agagcatcag catcatcgac accccgggta tcctgtcggg tgccaagcag agagtgagcc
661 gcggctacga cttcccggcc gtgctgcgct ggttcgcgga gcgcgtggac ctcatcatcc
721 tgctctttga tgcgcacaag ctggagatct cggacgagtt ctcagaggcc atcggcgcgt
781 tgcggggcca tgaggacaag atccgcgtgg tgctcaacaa ggccgacatg gtggagacgc
841 agcagctgat gcgcgtctac ggcgcgctca tgtgggcgct gggcaaggtg gtgggcacgc
901 ccgaggtgct gcgcgtctac atcggctcct tctggtccca gcccctcctc gtgcccgaca
961 accggcgcct cttcgagctg gaggagcagg acctcttccg cgacatccag ggcctgcccc
1021 ggcacgcagc cttgcgcaag ctcaacgacc tggtgaagag ggcccggctg gtgcgagttc
1081 acgcttacat catcagctac ctgaagaagg agatgccctc tgtgtttggg aaggagaaca
1141 agaagaagca gctgatcctc aaactgcccg tcatctttgc gaagattcag ctggaacatc
1201 acatctcccc tggggacttt cctgattgcc agaaaatgca ggagctgctg atggcgcatg
1261 acttcaccaa gtttcactcg ctgaagccga agctgctgga ggcactggac gagatgctga
1321 cgcacgacat cgccaagctc atgcccctgc tgcggcagga ggagctggag agcaccgagg
1381 tgggcgtgca ggggggcgct tttgagggca cccacatggg cccgtttgtg gagcggggac
1441 ctgacgaggc catggaggac ggcgaggagg gctcggacga cgaggccgag tgggtggtga
1501 ccaaggacaa gtccaaatac gacgagatct tctacaacct ggcgcctgcc gacggcaagc
1561 tgagcggctc caaggccaag acctggatgg tggggaccaa gctccccaac tcagtgctgg
1621 ggcgcatctg gaagctcagc gatgtggacc gcgacggcat gctggatgat gaagagttcg
1681 cgctggccag ccacctcatc gaggccaagc tggaaggcca cgggctgccc gccaacctgc
1741 cccgtcgcct ggtgccaccc tccaagcgac gccacaaggg ctccgccgag tgagccgggc
1801 ccccctccca tggccctgct gtggctcccc agctccagtc ggctgcacgc acacccctgc
1861 tccggctcac acacgccctg cctgccctcc ctgcccagct gtaaggaccg ggggtctccc
1921 tcctcactac cgccagacac cccggtggaa gcatttagag gggaccacgg gagggacaag
1981 gcttctctgt ccgcccttca cacctccagc ctcacgttca cttaggcaca tcacacacac
2041 actggcacac gcaggcatcc atccatccgt cattcattca aatatttatt gagcacctac
2101 tatgtgccca gccctgttct aggcactggg cattaccata gagaacaaaa tagacaaata
2161 catctgccct catggaaggt gacgttccca ggagagggca cctacacagt cacgcaaaca
2221 cacactaatt cctggcaggg cccccagccc ctcccctggc tgagcagccc tgtggctgaa
2281 atgactagca gataaacaga cccccttctg ctccgcttcc tcctgcccag ccaggcaaca
2341 ccctcaaccg gctccatcac atcctcaggt ctcgggacca tggggggctc agaggggaga
2401 cacacctact gcttcctcag atgggcccct ccgcagcccc ttcccttgct cggggaaagc
2461 ccccaattct gcccacaccc atttatttcc ttccttcctt ccttcttttc tttccttcct
2521 tccttctttt ttgtttttgc ccccaattct gcccataccc atttctttct ttccttcctt
2581 ccttcttttt tgtttttgcc cccagttctg tccacacccc ttccctttcc tgtcctgtcc
2641 tttctttctt tcttttttga tagaatcttg ctctgtcgcc caggctggag tgcagtggtg
2701 agatctcagc tcactgcaac ctccacctcc tgggttgaag tgattctcgt gcctcagcct
2761 cctgagtagc tgggactgca ggcacgcgcc accacgccca gctaattttt gtatttgagt
2821 agagacgggg tttcaccatg ttggccaggc tggtctcgaa ctccgcatct caggtgatct
2881 gctcgcctcg gcctcccaaa gtgatgggat tacaggcatg agccaccgtg cccggcttca
2941 cacccatttc tttaaaaagg atcccgtagc aggcagaaaa gccccttcca tcctgctcct
3001 ctgatactgt gcccccttgg agatatttcc gtcctccacc cacgtgtctg tggctggaac
3061 tgcccagcct gctcctggcc ccctggaagc ctccccacag ctggtaatct ggacttaagg
3121 attgctgggc caccgcctct ctgcctacca ccattccata tttaagtgga gcccctacgt
3181 agaaaggccc cggggcttta ttttagtctc cttttcaggg atgtcgtggg cgggggaggg
3241 ggttcttggt gctacagccc tctccccacc cctaaaggga cgccgacgct gtttgctgcc
3301 ttcaccacat attagtgctt gaccctggca ggggacccca tggaaaagat ggggaagagc
3361 aaaatacatg gagacgacgc accctccagg atgctcgctg ggattcccac gcccaccact
3421 gtcccccacc ccatggctgg gaggggcctc tgaacggaac agtgtcccca cagagcgaat
3481 aaagccaagg cttcttccca aaaaaaaaaa aaaaaaa
//