LOCUS BC014217 1474 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens leucine carboxyl methyltransferase 1, mRNA (cDNA clone
MGC:20616 IMAGE:3959936), complete cds.
ACCESSION BC014217
VERSION BC014217.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1474)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1474)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC014217.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 30 Row: b Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7705579
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1474
/db_xref="H-InvDB:HIT000036582"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20616 IMAGE:3959936"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1474
/gene="LCMT1"
/gene_synonym="CGI-68"
/gene_synonym="LCMT"
/gene_synonym="PPMT1"
/db_xref="GeneID:51451"
/db_xref="HGNC:HGNC:17557"
/db_xref="MIM:610286"
CDS 178..1251
/gene="LCMT1"
/gene_synonym="CGI-68"
/gene_synonym="LCMT"
/gene_synonym="PPMT1"
/codon_start=1
/product="LCMT1 protein"
/protein_id="AAH14217.1"
/db_xref="GeneID:51451"
/db_xref="HGNC:HGNC:17557"
/db_xref="MIM:610286"
/translation="MLPCARELPSAAPDSTLLLTAQHWVANLFILATLIGWTVRSFFL
GRMETCCSHLGLRSGRRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGYFARVHGVSQ
LIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCK
PPLSSPILELHSEDTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLL
IAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLA
GVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLEFLDEMELLEQL
MRHYCLCWATKGGNELGLKEITY"
BASE COUNT 388 a 376 c 379 g 331 t
ORIGIN
1 ctgtggctcg cgccgtcccc cgccgcccgt cgaccccgct tccatgtccc tggcggacac
61 agctcccagg aacctccacg cccatggcca ctaggcagag ggaatcctct atcacctcct
121 gctgttccac ctcgagctgc gacgcagacg acgagggcgt gcgcggcacc tgcgaagatg
181 cttccctgtg caagagaact cccatcagca gcgcccgaca gcactctcct cctcactgcc
241 caacactggg tggcaaatct tttcatcttg gccactctga taggctggac agtgaggtcc
301 ttcttcctag ggcgcatgga aacctgttgc agtcacctgg gtttgcggag tgggaggagg
361 tttgcagtaa gcattggcta ctggcatgac ccttacatac agcactttgt gagactgtct
421 aaagagagga aagcccctga aatcaacaga ggatattttg ctcgagtcca tggtgtcagt
481 cagcttataa aggcatttct acggaagaca gaatgtcatt gtcaaattgt caaccttggg
541 gcaggcatgg ataccacctt ctggagatta aaggatgaag atcttctccc aagtaaatat
601 tttgaggttg actttccaat gattgtcacg agaaagctgc acagtatcaa atgcaagcct
661 cccctatcca gccccattct agaactgcat tcagaggaca cacttcagat ggatggacac
721 atactggatt caaagagata tgccgttatt ggagcagatc tccgagacct gtctgaactg
781 gaagagaagc taaagaaatg taacatgaat acacaattgc caacactcct gatagctgaa
841 tgtgtgctgg tttacatgac tccagagcag tccgcaaacc tcctgaagtg ggcagccaac
901 agttttgaga gagccatgtt cataaactac gaacaggtga acatgggtga tcggtttggg
961 cagatcatga ttgaaaacct gcggagacgc cagtgtgacc tggcgggagt ggagacctgc
1021 aagtcattag agtcacagaa agaacggctc ctgtcgaatg ggtgggaaac agcatcggcc
1081 gtcgacatga tggagttgta caacaggtta cctcgagctg aagtgagcag gatagaatca
1141 cttgaattcc tggatgaaat ggagctgctg gagcagctca tgcggcatta ctgcctttgc
1201 tgggcaacca aaggaggaaa tgagcttggg ctgaaggaga taacttatta atctgtcgaa
1261 ggcttatgcc gagccagaag ccgaagccac ttgccctcct ggaggagacc tgcaagctcc
1321 ctgagcggtg ggcgggcctc gtccgcaggt ctcatcccac actcttgaga agccttggtc
1381 actacagtgg tcgcacatgt tcctcttcct gttcctgttg acatgtcgtt gtttaaataa
1441 atctcacttg ccaccaaaaa aaaaaaaaaa aaaa
//