LOCUS BC014184 1685 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens paraspeckle component 1, mRNA (cDNA clone MGC:20640
IMAGE:4623069), complete cds.
ACCESSION BC014184
VERSION BC014184.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1685)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1685)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC014184.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 30 Row: m Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 8922788
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1685
/db_xref="H-InvDB:HIT000036566"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20640 IMAGE:4623069"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1685
/gene="PSPC1"
/gene_synonym="PSP1"
/db_xref="GeneID:55269"
/db_xref="HGNC:HGNC:20320"
/db_xref="MIM:612408"
CDS 156..1337
/gene="PSPC1"
/gene_synonym="PSP1"
/codon_start=1
/product="PSPC1 protein"
/protein_id="AAH14184.2"
/db_xref="GeneID:55269"
/db_xref="HGNC:HGNC:20320"
/db_xref="MIM:612408"
/translation="MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGE
PAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLF
ERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTV
KNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGD
GAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYA
SRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRL
EELRNQELQKRKQIQLRHEEEHRRREEEMIRHREQEELRRQQEGFKPNYMENGDKRKC
G"
BASE COUNT 520 a 365 c 446 g 354 t
ORIGIN
1 gttgggggag gacgggttgc cgactcgcct acctagcggt ctcttgattg tcgatatttt
61 gttggcatag gtttatgtag agacgtatac atatatatag acacactgtc tttaaatcta
121 ggcctgtatc cggtgtccga ggcgaactca gtaagatgat gttaagagga aacctgaagc
181 aagtgcgcat tgagaaaaac ccggcccgcc ttcgcgccct ggagtccgcg gtgggcgaga
241 gcgagccggc ggccgcggca gccatggcgc tcgctcttgc cggggagccg gcaccgcccg
301 cgcccgcgcc tccagaggac cacccggacg aggagatggg gttcactatc gacatcaaga
361 gtttcctcaa gccgggcgag aagacgtaca cgcagcgctg ccgcctcttc gtgggaaatc
421 tgcccaccga catcacggag gaggacttca agaggctctt cgaacgctat ggcgagccca
481 gcgaagtctt catcaaccgg gaccgtggct tcggcttcat ccgcttggaa tccagaaccc
541 tggctgaaat tgcaaaagca gagctggacg gcaccattct caagagcaga cctctacgga
601 ttcgcttcgc tacacatgga gcagccttga ctgtcaagaa cctttctcca gttgtttcca
661 atgagctgct agagcaagca ttttctcagt ttggtccagt agagaaagct gttgtggttg
721 tggatgatcg cggtagagct acaggaaaag gttttgtaga gtttgcagca aaacctcctg
781 cacgaaaggc tctggaaaga tgtggtgatg gggcattctt gctaacaacg acccctcgtc
841 cagtcattgt ggaacccatg gagcagtttg atgatgaaga tggcttgcca gagaagctga
901 tgcagaaaac tcaacaatat cataaggaaa gagaacaacc accacgtttt gctcaacctg
961 ggacatttga atttgagtat gcatctcgat ggaaggctct tgatgaaatg gaaaagcagc
1021 agcgtgagca ggttgataga aacatcagag aagccaaaga gaaactggag gcagaaatgg
1081 aagcagctag gcatgaacac caattaatgc taatgaggca agatctaatg aggcgtcaag
1141 aagaactcag acgcttggaa gaactcagaa accaagagtt gcaaaaacgg aagcaaatac
1201 aactaagaca tgaagaggag catcggcggc gtgaggaaga aatgatccga cacagagaac
1261 aggaggaact gaggcgacag caagagggct ttaagccaaa ctacatggaa aatggtgata
1321 aaagaaaatg tggctgaagt tacccgaagt tcagcttgca gtgtaattca gaagagttaa
1381 gaccagaagt actggaatca tacttccttt cagtcttgcc acttgcctag tgaggctttt
1441 ccgaacctgg aaggagacgt ctagaaatcc caactttgct gtgtaaggac cattagctgc
1501 aagtcagtgg aagtctatag aaagcagtgt gaattccata gtggtcttga cttcttagca
1561 aggatttggg ataagtctaa aacttcagac ttaaagcttt gaagcatgtt aactgcatga
1621 aatattaaac atttcgaact tcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1681 aaaaa
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