LOCUS BC014125 1284 bp mRNA linear HUM 23-DEC-2003
DEFINITION Homo sapiens splicing factor 3b, subunit 2, 145kDa, mRNA (cDNA
clone IMAGE:4562562), partial cds.
ACCESSION BC014125
VERSION BC014125.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1284)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1284)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (10-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 19, 2003 this sequence version replaced BC014125.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 30 Row: i Column: 19.
FEATURES Location/Qualifiers
source 1..1284
/db_xref="H-InvDB:HIT000088289"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4562562"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..1284
/gene="SF3B2"
/gene_synonym="SAP145"
/gene_synonym="SF3b1"
/gene_synonym="SF3B145"
/gene_synonym="SF3b150"
/db_xref="GeneID:10992"
/db_xref="MIM:605591"
CDS <1..1116
/gene="SF3B2"
/gene_synonym="SAP145"
/gene_synonym="SF3b1"
/gene_synonym="SF3B145"
/gene_synonym="SF3b150"
/codon_start=1
/product="SF3B2 protein"
/protein_id="AAH14125.2"
/db_xref="GeneID:10992"
/db_xref="MIM:605591"
/translation="PDFIKRTGIQEMREALQEKEEQKTMKSKMREKVRPKMGKIDIDY
QKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRLKEKKPGDLSDELRISLGMPVGPNAH
KVPPPWLIAMQRYGPPPSYPNLKIPGLNSPIPESCSFGYHAGGWGKPPVDETGKPLYG
DVFGTNAAEFQTKTEEEEIDRTPWGELEPSDEESSEEEEEEESDEDKPDETGFITPAD
SGLITPGGFSSVPAGMETPELIELRKKKIEEAMDGSETPQLFTVLPEKRTATVGGAMM
GSTHIYDMSTVMSRKGPAPELQGVEVALAPEELELDPMAMTQKYEEHVREQQAQVEKE
DFSDMVAEHAAKQKQKKRKAQPQDSRGGSKKYKEFKF"
misc_feature 1..219
/gene="SF3B2"
/gene_synonym="SAP145"
/gene_synonym="SF3b1"
/gene_synonym="SF3B145"
/gene_synonym="SF3b150"
/note="DUF382; Region: Domain of unknown function
(DUF382). This domain is specific to the human splicing
factor 3b subunit 2 and it's orthologues. Splicing factor
3b subunit 2 or SAP145 is a suppressor of U2 snRNA
mutations. Pre-mRNA splicing is catalysed by a large
ribonucleoprotein complex called the spliceosome.
Spliceosomes are multi-component enzymes that catalyse
pre-mRNA splicing and form step-wise by the ordered
interaction of UsnRNPs and non-snRNP proteins with short
conserved regions of the pre-mRNA at the 5' and 3' splice
sites and branch site"
/db_xref="CDD:pfam04037"
misc_feature 229..405
/gene="SF3B2"
/gene_synonym="SAP145"
/gene_synonym="SF3b1"
/gene_synonym="SF3B145"
/gene_synonym="SF3b150"
/note="BASIC; Region: Basic domain in HLH proteins of MYOD
family"
/db_xref="CDD:smart00520"
BASE COUNT 380 a 297 c 358 g 249 t
ORIGIN
1 ccagacttca tcaaacgcac aggcatccag gagatgcgag aggccctgca ggagaaggaa
61 gaacagaaga ccatgaagtc aaaaatgcga gagaaagttc ggcctaagat gggcaaaatt
121 gacatcgact accagaaact gcatgatgcc ttcttcaagt ggcagaccaa gccaaagctg
181 accatccatg gggacctgta ctatgagggg aaggagttcg agacacgact gaaggagaag
241 aagccaggag atctgtctga tgagctaagg atttccttgg ggatgccagt aggaccaaat
301 gcccacaagg tccctccccc atggctgatt gccatgcagc gatatggacc acccccatcg
361 tatcccaacc tgaaaatccc tgggctgaac tcgcccatcc ctgagagctg ttcctttggg
421 taccatgctg gtggctgggg caaacctcca gtggatgaga ctgggaaacc gctctatggg
481 gacgtgtttg gaaccaatgc tgctgaattt cagaccaaga ctgaggaaga agagattgat
541 cggacccctt ggggggaact ggaaccatct gatgaagaat cctcagaaga agaggaagag
601 gaagaaagtg atgaagacaa accagatgag acaggcttta ttacccctgc agacagtggc
661 cttatcactc ctggaggctt ttcatcagtg cctgctggaa tggagacccc tgaactcatt
721 gagctgagga agaagaagat tgaggaggcg atggacggaa gtgagacacc tcagctcttc
781 actgtgttgc cagagaagag aacagccact gttggagggg ccatgatggg atcaacccac
841 atttatgaca tgtccacggt tatgagccgg aagggcccgg ctcctgagct gcaaggtgtg
901 gaagtggcgc tggcgcctga agagttggag ctggatccta tggccatgac ccagaagtat
961 gaggagcatg tgcgggagca gcaggctcaa gtagagaagg aggacttcag tgacatggtg
1021 gctgagcacg ctgccaaaca gaagcaaaaa aaacggaaag ctcagcccca ggacagccgt
1081 gggggcagca agaaatataa ggagttcaag ttttaggtcc cctcacacta gccctttttt
1141 tggccctacg tctggatgcc tgggcttcac acaagaacca cctctcccgc agttcccaag
1201 gacttgtcat ttcatgttct tattttagac ctgttttgta aataaagctg tttcccaagg
1261 aaaaaaaaaa aaaaaaaaaa aaaa
//