LOCUS BC014062 2242 bp mRNA linear HUM 23-DEC-2003
DEFINITION Homo sapiens DnaJ (Hsp40) homolog, subfamily A, member 3, mRNA
(cDNA clone IMAGE:3845144), partial cds.
ACCESSION BC014062
VERSION BC014062.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2242)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2242)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (10-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 19, 2003 this sequence version replaced BC014062.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 28 Row: f Column: 12.
FEATURES Location/Qualifiers
source 1..2242
/db_xref="H-InvDB:HIT000088269"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3845144"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2242
/gene="DNAJA3"
/gene_synonym="hTid-1"
/gene_synonym="TID1"
/db_xref="GeneID:9093"
CDS <1..1355
/gene="DNAJA3"
/gene_synonym="hTid-1"
/gene_synonym="TID1"
/codon_start=3
/product="DNAJA3 protein"
/protein_id="AAH14062.1"
/db_xref="GeneID:9093"
/translation="RCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPA
FASSLTSCGPRALLTLRPGVSLTGTKHYPFICTASFHTSAPLAKEDYYQILGVPRNAS
QKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFD
PGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAA
KGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCG
GRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSP
VFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPR
INSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGKRSTGN
"
misc_feature 270..467
/gene="DNAJA3"
/gene_synonym="hTid-1"
/gene_synonym="TID1"
/note="DnaJ; Region: DnaJ domain. DnaJ domains (J-domains)
are associated with hsp70 heat-shock system and it is
thought that this domain mediates the interaction.
DnaJ-domain is therefore part of a chaperone (protein
folding) system. The T-antigens are confirmed as DnaJ
containing domains from literature"
/db_xref="CDD:pfam00226"
misc_feature 663..896
/gene="DNAJA3"
/gene_synonym="hTid-1"
/gene_synonym="TID1"
/note="DnaJ_CXXCXGXG; Region: DnaJ central domain (4
repeats). The central cysteine-rich (CR) domain of DnaJ
proteins contains four repeats of the motif CXXCXGXG where
X is any amino acid. The isolated cysteine rich domain
folds in zinc dependent fashion. Each set of two repeats
binds one unit of zinc. Although this domain has been
implicated in substrate binding, no evidence of specific
interaction between the isolated DNAJ cysteine rich domain
and various hydrophobic peptides has been found"
/db_xref="CDD:pfam00684"
misc_feature 912..1277
/gene="DNAJA3"
/gene_synonym="hTid-1"
/gene_synonym="TID1"
/note="DnaJ_C; Region: DnaJ C terminal region. This family
consists of the C terminal region form the DnaJ protein.
Although the function of this region is unknown, it is
always found associated with pfam00226 and pfam00684. DnaJ
is a chaperone associated with the Hsp70 heat-shock system
involved in protein folding and renaturation after stress"
/db_xref="CDD:pfam01556"
BASE COUNT 516 a 605 c 650 g 471 t
ORIGIN
1 cgcggtgctc cacacgctgg ttgctggtgg ttgtggggac cccgcggctg ccggctatat
61 cgggtagagg ggcccggccg cccagggagg gcgtggtggg ggcatggctg agccgcaagc
121 tgagcgtccc cgcctttgcg tcttccctga cctcttgcgg cccccgagcg ctgctgacat
181 tgagacctgg tgtcagcctc acaggaacaa aacattaccc tttcatttgt actgcctcct
241 tccacacgag tgcccctttg gccaaagaag attattatca gatattagga gtgcctcgaa
301 atgccagcca gaaagagatc aagaaagcct attatcagct tgccaagaag tatcaccctg
361 acacaaataa ggatgatccc aaagccaagg agaagttctc ccagctggca gaagcctatg
421 aggttttgag tgatgaggtg aagaggaagc agtacgatgc ctacggctct gcaggcttcg
481 atcctggggc cagcggctcc cagcatagct actggaaggg aggccccact gtggaccccg
541 aggagctgtt caggaagatc tttggcgagt tctcatcctc ttcatttgga gatttccaga
601 ccgtgtttga tcagcctcag gaatacttca tggagttgac attcaatcaa gctgcaaagg
661 gggtcaacaa ggagttcacc gtgaacatca tggacacgtg tgagcgctgc aacggcaagg
721 ggaacgagcc cggcaccaag gtgcagcatt gccactactg tggcggctcc ggcatggaaa
781 ccatcaacac aggccctttt gtgatgcgtt ccacgtgtag gagatgtggt ggccgcggct
841 ccatcatcat atcgccctgt gtggtctgca ggggagcagg acaagccaag cagaaaaagc
901 gagtgatgat ccctgtgcct gcaggagtcg aggatggcca gaccgtgagg atgcctgtgg
961 gaaaaaggga aattttcatt acgttcaggg tgcagaaaag ccctgtgttc cggagggacg
1021 gcgcagacat ccactccgac ctctttattt ctatagctca ggctcttctt gggggaacag
1081 ccagagccca gggcctgtac gagacgatca acgtgacgat cccccctggg actcagacag
1141 accagaagat tcggatgggt gggaaaggca tcccccggat taacagctac ggctacggag
1201 accactacat ccacatcaag atacgagttc caaagaggct aacgagccgg cagcagagcc
1261 tgatcctgag ctacgccgag gacgagacag atgtggaggg gacggtgaac ggcgtcaccc
1321 tcaccagctc tggaaaaaga tccactggaa actaggccgg gaagcagcag cccctccaag
1381 ggccagggca cctgggagac gggaggattc cagaacagca gcactgagct cccacccgca
1441 gagcctctgg acggccttgg caacagcaaa atcatgggac aacacctctc tccacggaaa
1501 ggtcacagtg gacagcccgg gcagtaggat gcagccccag aggctggtgg cagtttcctg
1561 tccattggta ggtgacggcc cctggctcag gcagagggag atggttagac tcttgcaggg
1621 ctaaaactct aatttggaat tgaatattgt ggatatctta gttaaaggcc atgcttacag
1681 cttagaaatg aagccttaag ctgcatcaag ttacgaagtg attaatttcc ttctcagcaa
1741 acctccggga ggttccagaa tgagttcttc ctgacaggtt gtcttcactg ggagcgtggg
1801 gcccccaggc cccaccagca ccgtcctccc ctaatgaggg gccctgccga ggcatcagct
1861 gctctgctca gttagttttt attcccgggg taccaagcag ctgcacagtc ggtgcctggg
1921 aggcacgtag aggcccagag agtccctggg ggttctgctc tgaccgtgtg ggtggtgatc
1981 cttgtcagga tgtacagtcc ttgctcccac cccatccagg atggccgcct gtccctgact
2041 attgagtcct gttgttgtaa gccaggcatg gagggctcct gcccttctgc tgagccacag
2101 cccattgcag cactgtgctg gccagacttc agctgccttg ggaactgaag ccctgccact
2161 gttgctagtc aggggcttgg ttctcccact tacactgttg acatctattt tctgaagtgt
2221 gtttaaatta ttcagtgcta at
//