LOCUS BC014036 4510 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphorylase kinase, alpha 2 (liver), mRNA (cDNA
clone MGC:20154 IMAGE:4547507), complete cds.
ACCESSION BC014036
VERSION BC014036.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4510)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4510)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC014036.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 28 Row: p Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4505780.
FEATURES Location/Qualifiers
source 1..4510
/db_xref="H-InvDB:HIT000036481"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20154 IMAGE:4547507"
/tissue_type="Colon, adenocarcinoma"
/clone_lib="NIH_MGC_15"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..4510
/gene="PHKA2"
/gene_synonym="PYKL"
/gene_synonym="XLG"
/gene_synonym="XLG2"
/db_xref="GeneID:5256"
/db_xref="HGNC:HGNC:8926"
/db_xref="MIM:306000"
CDS 218..3925
/gene="PHKA2"
/gene_synonym="PYKL"
/gene_synonym="XLG"
/gene_synonym="XLG2"
/codon_start=1
/product="phosphorylase kinase, alpha 2 (liver)"
/protein_id="AAH14036.1"
/db_xref="GeneID:5256"
/db_xref="HGNC:HGNC:8926"
/db_xref="MIM:306000"
/translation="MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHEQKDAWV
RDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVAKVEKF
KHTQSTKDSLHAKYNTATCGTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLD
EVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLF
GAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLV
NVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTY
FIIDGVFSGDAVQVQEYREALEGILIRGKNGIRLVPELYAVPPNKVDEEYKNPHTVDR
VPMGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNH
IKDLLRKHGVNVQSIADIHPIQVQPGRILSHIYAKLGRNKNMNLSGRPYRHIGVLGTS
KLYVIRNQIFTFTPQFTDEHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPI
SRTMLTNDGSDIHSAVLSTIRKLEDGYFGGARVKLGNLSEFLTTSFYTYLTFLDPDCD
EKLFDNASEGTFSPDSDSDLVGYLEDTCNQESQDELDHYINHLLQSTSLRSYLPPLCK
NTEDRHVFSAIHSTRDILSVMAKAKGLEVPFVPMTLPTKVLSAHRKSLNLVDSPQPLL
EKVPESDFQWPRDDHSDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQ
HGVTVQNLLGELYGKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLP
PEPREKIISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLR
LRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSVRP
IHSSTSSPTISIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQFFSVGQAASSS
AHSSKSARSSTPSSPTGTSSSDSGGHHIGWGERQGQWLRRRRLDGAINRVPVGFYQRV
WKILQKCHGLSIDGYVLPSSTTREMTPHEIKFAVHVESVLNRVPQPEYRQLLVEAIMV
LTLLSDTEMTSIGGIIHVDQIVQMASQLFLQDQVSIGAMDTLEKDQATGICHFFYDSA
PSGAYGTMTYLTRAVASYLQELLPNSGCQMQ"
BASE COUNT 1160 a 1151 c 1172 g 1027 t
ORIGIN
1 cgcgcctccc cgccgcgcgg agctctggtt ggcttgcttt ccaaccggac tttggggcta
61 gcgtttagaa aagtctcgac ctcctccggc ccggtcccat cccaagaacc gactaaggct
121 gtgagtgtcc gggaaccaga cccgcttgga ggccacagcc ccgacgtccc gcgcccacgc
181 ggcagatcgg gcgctgcggc ctgggagcct cggggagatg cggagcagga gcaattccgg
241 ggtccgcttg gacgggtacg cgcggctggt gcagcaaacc atcctgtgtt accagaatcc
301 cgtcacgggg ctgctgtcag ccagccatga gcagaaggat gcctgggtgc gggataacat
361 ctacagtatc ctggccgtgt ggggcctggg catggcctac cgtaagaatg cagaccgcga
421 tgaggacaag gccaaggcct acgagctgga gcagaacgtg gtgaagctga tgcgaggtct
481 tctccagtgc atgatgagac aggtggccaa agtggagaag ttcaaacaca ctcagagcac
541 caaggacagc ctgcacgcca agtacaacac cgccacctgt ggcacggtgg tgggcgacga
601 ccagtggggc cacctccagg tggatgccac ctctctcttc ctcctgttcc tggcccagat
661 gaccgcctca ggcttacgta tcattttcac tctcgatgag gtggccttca tacagaatct
721 tgtcttttac atagaagctg catataaagt cgctgattat ggaatgtggg agcgtggaga
781 taagactaat cagggcatcc cggaattgaa tgcaagctcc gtaggaatgg ccaaggcagc
841 tcttgaggca attgatgaac tggacctttt tggagcccat ggaggacgca agtcagtgat
901 tcatgttctg ccagatgagg tcgagcactg ccagtctatt ctgttctcca tgctgccaag
961 agcgtcgaca tctaaagaaa ttgatgctgg acttctttcc attatttcct tcccggcctt
1021 tgcagtggaa gatgtaaacc ttgtaaatgt gaccaaaaat gaaattattt ctaagctcca
1081 ggggcgttat ggatgctgtc gcttccttcg agatggttat aaaactccaa gagaggaccc
1141 taatcgactg cattatgacc ctgctgaact caagctcttc gaaaacattg aatgtgagtg
1201 gcctgtgttt tggacatatt ttataataga tggagtcttc agtggtgatg ctgttcaggt
1261 ccaagaatac cgagaggccc tggagggaat actcatcaga ggcaagaatg ggatccgcct
1321 ggtgcctgaa ctctacgctg tcccgcctaa caaggtagat gaagagtaca agaatcctca
1381 cacagtagac cgagttccta tggggaaggt gcctcatctg tggggccaat ccttgtacat
1441 cctcagctcg ctgttggcag agggattcct tgccgctggt gaaatcgatc ccttaaatag
1501 aagattttcc acttcagtca aacctgatgt tgtagtacaa gttactgttt tggcagaaaa
1561 caatcacatt aaggacttat tgaggaaaca cggggtgaac gtccagagta tcgcggacat
1621 tcatccaatt caagtccagc cgggccggat tcttagtcac atatatgcca agcttggacg
1681 gaataagaat atgaatttga gtgggcgacc gtatcgacat attggtgtcc ttggaacctc
1741 taaactatat gtgattagga accaaatctt tacttttaca ccccagttca ccgacgagca
1801 tcacttctac ctggccctcg acaatgagat gatcgtggag atgctaagga tcgagctggc
1861 ctacctgtgc acctgctgga ggatgacggg cagacccaca ctcaccttcc ccatcagtcg
1921 caccatgctc acaaatgatg gctcagacat tcattctgct gtgctctcca caattagaaa
1981 actagaggat ggatattttg gaggagccag agtaaaatta gggaaccttt cggaatttct
2041 caccacatcg ttctacacat atctgacttt tctggatcca gactgtgatg agaagttgtt
2101 tgacaatgcc agcgaaggga ctttcagtcc tgatagtgat tcagatttgg taggatatct
2161 ggaagacacc tgtaatcaag aaagccaaga cgaacttgac cattatatca accaccttct
2221 gcaaagcaca tcgttgaggt cctatctgcc tcctctttgt aagaacacag aagaccgcca
2281 tgtcttcagt gctatccact ccacgcggga catactttct gtgatggcaa aagcaaaggg
2341 tttggaagtt ccatttgttc ccatgacttt gccgactaaa gttctaagtg cccaccgtaa
2401 atcactgaat cttgttgatt ctcctcagcc actcctagaa aaggttcctg aaagtgactt
2461 tcagtggccc agagatgacc atagtgacgt ggactgtgag aagctggttg agcagctaaa
2521 agattgttcg aacctacagg accaagcaga cattctgtac attctttatg tcataaaggg
2581 tcccagctgg gacacaaatc tctctggaca gcacggggtc accgttcaaa accttcttgg
2641 tgagctctat gggaaagccg gcttgaacca ggagtggggt ctgattcgct acatctcagg
2701 ccttctcagg aagaaagtgg aggtcctggc tgaggcctgc acagacctgc tttcgcacca
2761 gaagcagctc accgtgggcc tgccgcccga gccccgggag aagatcatct ctgcgcccct
2821 tcccccagag gagctcacaa aactcatcta cgaggccagt gggcaggaca tcagcattgc
2881 cgtcctcacg caggagattg tggtttacct ggccatgtat gtcagggcgc agcccagcct
2941 ctttgtggag atgctgagac tccggattgg actgatcatt caggtgatgg ccacggagct
3001 ggcacggagc ctgaactgct caggagaaga ggcttctgaa agtttgatga acctcagccc
3061 tttcgatatg aaaaatctcc tgcaccatat tctaagtggg aaagagtttg gcgttgaaag
3121 aagtgtgcgc cctatccact cctccacatc cagccctacc atctccatcc acgaggtggg
3181 ccataccgga gtcaccaaaa ctgagaggag tggcattaac agactgagga gtgaaatgaa
3241 acagatgact aggcggttta gtgctgatga acagttcttt tctgtgggcc aggccgcgtc
3301 cagcagtgcg cattcctcca agtctgcgag gtccagcacc ccatcctcgc ccactggcac
3361 gtcatcctca gactcgggag gacatcacat cggctggggt gagcggcagg gccagtggct
3421 gcgcaggaga aggctggatg gggccatcaa cagggtcccc gtgggattct accagagggt
3481 gtggaagatc ctccagaagt gccacggtct ctccatcgat ggttatgtcc tcccatcctc
3541 gacgacccga gagatgaccc cgcatgagat caagtttgct gtccatgtcg aatcggtgct
3601 gaaccgcgtg ccgcagcccg agtaccggca gctgctggtg gaagccatca tggtgctgac
3661 gctgctctcg gacacggaga tgaccagcat cgggggcatc atccacgtgg accagatcgt
3721 gcagatggcc agtcagctgt tcttgcagga ccaggtgtca attggtgcca tggacaccct
3781 ggagaaagac caagccacag gaatctgcca cttcttttat gacagcgctc cgagtggggc
3841 ttatgggacg atgacctacc taacaagagc agtggcttct tatttgcagg aattgttgcc
3901 caattcgggc tgccagatgc aatagggtct cacctggaaa catgatcaca ctctcaatct
3961 gtcacgtgcc ccctagcctt actgggaacc ttctgtcccc caagatcccc tgtgctatca
4021 ggaaagcatg tcccatcaga aacactctcg gggggcaatg gtagcactca ccctgaaact
4081 gatgtatgtt aaagccacag agatagagct gaggagtcct gtgttccccc gcaaggagca
4141 ccccgggatc attttctagg ttcatttctc tggaacattt gctgtagcat ctggtctcac
4201 ggactctgag gaggaattgg aaattggtct cttttgagtg cagagggaac tgagacgcca
4261 gcttaaattg gctcttgcag agagttacag aaatagtttc gatgagctag tgacacatcc
4321 taaagatgca aagatcctcc tggcggcagt agccttgaca agggccacct cttcacagga
4381 tgcagtctgt ctgtgcacca aactcttcac caaatagaac acttgtgtct ctctgtgaag
4441 aagaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
4501 aaaaaaaaaa
//