LOCUS BC013975 2816 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens sema domain, immunoglobulin domain (Ig), short basic
domain, secreted, (semaphorin) 3B, mRNA (cDNA clone MGC:12697
IMAGE:4121913), complete cds.
ACCESSION BC013975
VERSION BC013975.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2816)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2816)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC013975.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 17 Row: f Column: 20
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 54607087.
FEATURES Location/Qualifiers
source 1..2816
/db_xref="H-InvDB:HIT000036439"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:12697 IMAGE:4121913"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2816
/gene="SEMA3B"
/gene_synonym="LUCA-1"
/gene_synonym="SemA"
/gene_synonym="SEMA5"
/gene_synonym="semaV"
/db_xref="GeneID:7869"
/db_xref="HGNC:HGNC:10724"
/db_xref="MIM:601281"
CDS 73..2319
/gene="SEMA3B"
/gene_synonym="LUCA-1"
/gene_synonym="SemA"
/gene_synonym="SEMA5"
/gene_synonym="semaV"
/codon_start=1
/product="sema domain, immunoglobulin domain (Ig), short
basic domain, secreted, (semaphorin) 3B"
/protein_id="AAH13975.1"
/db_xref="GeneID:7869"
/db_xref="HGNC:HGNC:10724"
/db_xref="MIM:601281"
/translation="MGRAGAAAVIPGLALLWAVGLGSAAPSPPRLRLSFQELQAWHGL
QTFSLERTCCYQALLVDEERGRLFVGAENHVASLNLDNISKRAKKLAWPAPVEWREEC
NWAGKDIGTECMNFVKLLHAYNRTHLLACGTGAFHPTCAFVEVGHRAEEPVLRLDPGR
IEDGKGKSPYDPRHRAASVLVGEELYSGVAADLMGRDFTIFRSLGQRPSLRTEPHDSR
WLNEPKFVKVFWIPESENPDDDKIYFFFRETAVEAAPALGRLSVSRVGQICRNDVGGQ
RSLVNKWTTFLKARLVCSVPGVEGDTHFDQLQDVFLLSSRDHRTPLLYAVFSTSSIFQ
GSAVCVYSMNDVRRAFLGPFAHKEGPMHQWVSYQGRVPYPRPGMCPSKTFGTFSSTKD
FPDDVIQFARNHPLMYNSVLPTGGRPLFLQVGANYTFTQIAADRVAAADGHYDVLFIG
TDVGTVLKVISVPKGSRPSAEGLLLEELHVFEDSAAVTSMQISSKRHQLYVASRSAVA
QIALHRCAAHGRVCTECCLARDPYCAWDGVACTRFQPSAKRRFRRQDVRNGDPSTLCS
GDSSRPALLEHKVFGVEGSSAFLECEPRSLQARVEWTFQRAGVTAHTQVLAEERTERT
ARGLLLRRLRRRDSGVYLCAAVEQGFTQPLRRLSLHVLSATQAERLARAEEAAPAAPP
GPKLWYRDFLQLVEPGGGGSANSLRMCRPQPALQSLPLESRRKGRNRRTHAPEPRAER
GPRSATHW"
BASE COUNT 572 a 865 c 919 g 460 t
ORIGIN
1 gcagagtcca gggcagctca aggctcctcc acacacacac ccgctgaacc ctgagcaccc
61 tgagctgctg agatggggcg ggccggggct gccgccgtga tcccgggcct ggccctgctc
121 tgggcagtgg ggctggggag tgccgccccc agccccccac gccttcggct ctccttccaa
181 gagctccagg cctggcatgg tctccagact ttcagcctgg agcgaacctg ctgctaccag
241 gccttgctgg tggatgagga gcgtggacgc ctgtttgtgg gtgccgagaa ccatgtggcc
301 tccctcaacc tggacaacat cagcaagcgg gccaagaagc tggcctggcc ggcccctgtg
361 gaatggcgag aggagtgcaa ctgggcaggg aaggacattg gtactgagtg catgaacttc
421 gtgaagttgc tgcatgccta caaccgcacc catttgctgg cctgtggcac gggagccttc
481 cacccaacct gtgcctttgt ggaagtgggc caccgggcag aggagcccgt cctccggctg
541 gacccaggaa ggatagagga tggcaagggg aagagtcctt atgaccccag gcatcgggct
601 gcctccgtgc tggtggggga ggagctatac tcaggggtgg cagcagacct catgggacga
661 gactttacca tctttcgcag cctagggcaa cgtccaagtc tccgaacaga gccacacgac
721 tcccgctggc tcaatgagcc caagtttgtc aaggtatttt ggatcccgga gagcgagaac
781 ccagacgacg acaaaatcta cttcttcttt cgtgagacgg cggtagaggc ggcgccggca
841 ctgggacgcc tgtccgtgtc ccgcgttggc cagatctgcc ggaacgacgt gggcggccag
901 cgcagcctgg tcaacaagtg gacgacgttc ctgaaggcgc ggctggtgtg ctcggtgccc
961 ggcgtcgagg gcgacaccca cttcgatcag ctccaggatg tgtttctgtt gtcctcgcgg
1021 gaccaccgga ccccgctgct ctatgccgtc ttctccacgt ccagcatctt ccagggctct
1081 gcggtgtgcg tgtacagcat gaacgacgtg cgccgggcct tcttgggacc ctttgcacac
1141 aaggaggggc ccatgcacca gtgggtgtca taccagggtc gcgtccccta cccgcggcca
1201 ggcatgtgcc ccagcaagac ctttggcacc ttcagttcca ccaaggactt cccagacgat
1261 gtcatccagt ttgcgcggaa ccaccccctc atgtacaact ctgtcctgcc cactgggggg
1321 cgccctcttt tcctacaagt tggagccaat tacaccttca ctcaaattgc cgcggaccgg
1381 gttgcagccg ctgacggaca ctatgacgtc ctcttcattg gcacagacgt tggcacggtg
1441 ctgaaggtga tctcggtccc caagggcagt aggcccagcg cagaggggct gctcctggag
1501 gagctgcacg tgtttgagga ctcggccgct gtcaccagca tgcaaatttc ttccaagagg
1561 caccagctgt acgtagcctc gcggagcgcg gtggcccaga tcgcgttgca ccgctgcgct
1621 gcccacggcc gcgtctgcac cgaatgctgt ctggcgcgtg acccctactg cgcctgggac
1681 ggggtcgcgt gcacgcgctt ccagcccagt gccaagaggc ggttccggcg gcaagacgta
1741 aggaatggcg accccagcac gttgtgctcc ggagactcgt ctcgtcccgc gctgctggaa
1801 cacaaggtgt tcggcgtgga gggcagcagc gcctttctgg agtgtgagcc ccgctcgctg
1861 caggcgcgcg tggagtggac tttccagcgc gcaggggtga cagcccacac ccaggtgctg
1921 gcagaggagc gcaccgagcg caccgcccgg ggactactgc tgcgcaggct gcggcgccgg
1981 gactcgggcg tgtacttgtg cgccgccgtc gagcagggct ttacgcaacc gctgcgtcgc
2041 ctgtcgctgc acgtgttgag tgctacgcag gccgaacgac tggcgcgggc cgaggaggct
2101 gcgcccgccg cgccgccggg ccccaaactc tggtaccggg actttctgca gctggtggag
2161 ccgggcggag gtggcagcgc gaactccctg cgcatgtgcc gcccgcagcc tgcgctgcag
2221 tcactgcccc tggagtcgcg gagaaagggc cgtaaccgga ggacccacgc ccctgagcct
2281 cgcgctgagc gggggccgcg cagcgcaacg cactggtgac cagactgtcc ccacgccggg
2341 aaccaagcag gagacgacag gcgagagagg agccagacag accctgaaaa gaaggacggg
2401 ttggggccgg gcacattggg ggtcaccggc cgatggagac accaaccgac aggccctggc
2461 tgagggcagc tgcgcgggct tatttattaa caggataacc cttgaatgta gcagccccgg
2521 gagggcggca caggtcgggc gcaggattca gccggaggga agggacgggg aagccgagct
2581 ccagagcaac gaccagggcc gaggaggtgc ctggagtgcc caccctggga gacagacccc
2641 acctccttgg gtagtgagca gtgagcagaa agctgtgaac aggctgggct gctggaggtg
2701 gggcgaggca ggccgactgt actaaagtaa cgcaataaac gcattatcag ccaaaaaaaa
2761 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa
//