LOCUS BC013919 1569 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens thymidylate synthetase, mRNA (cDNA clone MGC:22884
IMAGE:4048625), complete cds.
ACCESSION BC013919
VERSION BC013919.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1569)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1569)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 31 Row: o Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: Similarity but not
identity to protein.
FEATURES Location/Qualifiers
source 1..1569
/db_xref="H-InvDB:HIT000036407"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:22884 IMAGE:4048625"
/tissue_type="Bone marrow, chronic myelogenous leukemia"
/clone_lib="NIH_MGC_54"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..1569
/gene="TYMS"
/gene_synonym="HsT422"
/gene_synonym="TMS"
/gene_synonym="TSase"
/db_xref="GeneID:7298"
/db_xref="HGNC:HGNC:12441"
/db_xref="MIM:188350"
CDS 96..1037
/gene="TYMS"
/gene_synonym="HsT422"
/gene_synonym="TMS"
/gene_synonym="TSase"
/codon_start=1
/product="thymidylate synthetase"
/protein_id="AAH13919.1"
/db_xref="GeneID:7298"
/db_xref="HGNC:HGNC:12441"
/db_xref="MIM:188350"
/translation="MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCG
VRKDDRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELS
SKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQ
LQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGD
MGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPF
PKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV"
BASE COUNT 425 a 368 c 398 g 378 t
ORIGIN
1 gggggcgcgc ggaaggggtc ctgccaccgc gccacttggc ctgcctccgt cccgccgcgc
61 cacttcgcct gcctccgtcc cccgcccgcc gcgccatgcc tgtggccggc tcggagctgc
121 cgcgccggcc cttgcccccc gccgcacagg agcgggacgc cgagccgcgt ccgccgcacg
181 gggagctgca gtacctgggg cagatccaac acatcctccg ctgcggcgtc aggaaggacg
241 accgcacggg caccggcacc ctgtcggtat tcggcatgca ggcgcgctac agcctgagag
301 atgaattccc tctgctgaca accaaacgtg tgttctggaa gggtgttttg gaggagttgc
361 tgtggtttat caagggatcc acaaatgcta aagagctgtc ttccaaggga gtgaaaatct
421 gggatgccaa tggatcccga gactttttgg acagcctggg attctccacc agagaagaag
481 gggacttggg cccagtttat ggcttccagt ggaggcattt tggggcagaa tacagagata
541 tggaatcaga ttattcagga cagggagttg accaactgca aagagtgatt gacaccatca
601 aaaccaaccc tgacgacaga agaatcatca tgtgcgcttg gaatccaaga gatcttcctc
661 tgatggcgct gcctccatgc catgccctct gccagttcta tgtggtgaac agtgagctgt
721 cctgccagct gtaccagaga tcgggagaca tgggcctcgg tgtgcctttc aacatcgcca
781 gctacgccct gctcacgtac atgattgcgc acatcacggg cctgaagcca ggtgacttta
841 tacacacttt gggagatgca catatttacc tgaatcacat cgagccactg aaaattcagc
901 ttcagcgaga acccagacct ttcccaaagc tcaggattct tcgaaaagtt gagaaaattg
961 atgacttcaa agctgaagac tttcagattg aagggtacaa tccgcatcca actattaaaa
1021 tggaaatggc tgtttagggt gctttcaaag gagctcgaag gatattgtca gtctttaggg
1081 gttgggctgg atgccgaggt aaaagttctt tttgctctaa aagaaaaagg aactaggtca
1141 aaaatctgtc cgtgacctat cagttattaa tttttaagga tgttgccact ggcaaatgta
1201 actgtgccag ttctttccat aataaaaggc tttgagttaa ctcactgagg gtatctgaca
1261 atgctgaggt tatgaacaaa gtgaggagaa tgaaatgtat gtgctcttag caaaaacatg
1321 tatgtgcatt tcaatcccac gtacttataa agaaggttgg tgaatttcac aagctatttt
1381 tggaatattt ttagaatatt ttaagaattt cacaagctat tccctcaaat ctgagggagc
1441 tgagtaacac catcgatcat gatgtagagt gtggttatga actttaaagt tatagttgtt
1501 ttatatgttg ctataataaa gaagtgttct gcattcgcca aaaaaaaaaa aaaaaaaaaa
1561 aaaaaaaaa
//