LOCUS BC013596 3041 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens glial fibrillary acidic protein, mRNA (cDNA clone
MGC:17031 IMAGE:4185584), complete cds.
ACCESSION BC013596
VERSION BC013596.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3041)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3041)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (04-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 12 Row: m Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 24430142.
FEATURES Location/Qualifiers
source 1..3041
/db_xref="H-InvDB:HIT000036299"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:17031 IMAGE:4185584"
/tissue_type="Brain, glioblastoma with EGFR amplification"
/clone_lib="NCI_CGAP_Brn64"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3041
/gene="GFAP"
/db_xref="GeneID:2670"
/db_xref="HGNC:HGNC:4235"
/db_xref="MIM:137780"
CDS 3..1301
/gene="GFAP"
/codon_start=1
/product="glial fibrillary acidic protein"
/protein_id="AAH13596.1"
/db_xref="GeneID:2670"
/db_xref="HGNC:HGNC:4235"
/db_xref="MIM:137780"
/translation="MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPP
PLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELN
QLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETN
LRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQ
QVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLR
QAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEE
GQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRET
SLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM"
BASE COUNT 679 a 865 c 925 g 572 t
ORIGIN
1 ggatggagag gagacgcatc acctccgctg ctcgccgctc ctacgtctcc tcaggggaga
61 tgatggtggg gggcctggct cctggccgcc gtctgggtcc tggcacccgc ctctccctgg
121 ctcgaatgcc ccctccactc ccgacccggg tggatttctc cctggctggg gcactcaatg
181 ctggcttcaa ggagacccgg gccagtgagc gggcagagat gatggagctc aatgaccgct
241 ttgccagcta catcgagaag gttcgcttcc tggaacagca aaacaaggcg ctggctgctg
301 agctgaacca gctgcgggcc aaggagccca ccaagctggc agacgtctac caggctgagc
361 tgcgagagct gcggctgcgg ctcgatcaac tcaccgccaa cagcgcccgg ctggaggttg
421 agagggacaa tctggcacag gacctggcca ctgtgaggca gaagctccag gatgaaacca
481 acctgaggct ggaagccgag aacaacctgg ctgcctatag acaggaagca gatgaagcca
541 ccctggcccg tctggatctg gagaggaaga ttgagtcgct ggaggaggag atccggttct
601 tgaggaagat ccacgaggag gaggttcggg aactccagga gcagctggcc cgacagcagg
661 tccatgtgga gcttgacgtg gccaagccag acctcaccgc agccctgaaa gagatccgca
721 cgcagtatga ggcaatggcg tccagcaaca tgcatgaagc cgaagagtgg taccgctcca
781 agtttgcaga cctgacagac gctgctgccc gcaacgcgga gctgctccgc caggccaagc
841 acgaagccaa cgactaccgg cgccagttgc agtccttgac ctgcgacctg gagtctctgc
901 gcggcacgaa cgagtccctg gagaggcaga tgcgcgagca ggaggagcgg cacgtgcggg
961 aggcggccag ttatcaggag gcgctggcgc ggctggagga agaggggcag agcctcaagg
1021 acgagatggc ccgccacttg caggagtacc aggacctgct caatgtcaag ctggccctgg
1081 acatcgagat cgccacctac aggaagctgc tagagggcga ggagaaccgg atcaccattc
1141 ccgtgcagac cttctccaac ctgcagattc gagaaaccag cctggacacc aagtctgtgt
1201 cagaaggcca cctcaagagg aacatcgtgg tgaagaccgt ggagatgcgg gatggagagg
1261 tcattaagga gtccaagcag gagcacaagg atgtgatgtg aggcaggacc cacctggtgg
1321 cctctgccgc gtctcatgag gggcccgagc agaagcagga tagttgctcc gcctctgctg
1381 gcacatttcc ccagacctga gctccccacc accccagctg ctcccctccc tcctctgtcc
1441 ctaggtcagc ttgctgccct aggctccgtc agtatcaggc ctgccagacg gcacccaccc
1501 agcacccagc aactccaact aacaagaaac tcacccccaa ggggcagtct ggaggggcat
1561 ggccagcagc ttgcgttaga atgaggagga aggagagaag gggaggaggg cggggggcac
1621 ctactacatc gccctccaca tccctgattc ctgttgttat ggaaactgtt gccagagatg
1681 gaggttctct cggagtatct gggaactgtg cctttgagtt tcctcaggct gctggaggaa
1741 aactgagact cagacaggaa agggaaggcc ccacagacaa ggtagccctg gccagaggct
1801 tgttttgtct tttggttttt atgaggtggg atatccctat gctgcctagg ctgaccttga
1861 actcctgggc tcaagcagtc tacccacctc agcctcctgt gtagctggga ttatagattg
1921 gagccaccat gcccagctca gagggttgtt ctcctagact gaccctgatc agtctaagat
1981 gggtggggac gtcctgccac ctggggcagt cacctgccca gatcccagaa ggacctcctg
2041 agcgatgact caagtgtctc agtccacctg agctgccatc cagggatgcc atctgtgggc
2101 acgctgtggg caggagggag cttgattctc agcacttggg ggatctgttg tgtacgtgga
2161 gagggatgag gtgctgggag ggatagaggg gggctgcctg gcccccagct gtgggtacag
2221 agaggtcaag cccaggagga ctgccccgtg cagactggag gggacgctgg tagagatgga
2281 ggaggaggca attgggatgg cgctaggcat acaagtaggg gttgtgggtg accagttgca
2341 cttggcctct ggattgtggg aattaaggaa gtgactcatc ctcttgaaga tgctgaaaca
2401 ggagagaaag gggatgtatc catgggggca gggcatgact ttgtcccatt tctaaaggcc
2461 tcttccttgc tgtgtcatac caggccgccc cagcctctga gcccctggga ctgctgcttc
2521 ttaaccccag taagccactg ccacacgtct gaccctctcc accccatagt gaccggctgc
2581 ttttccctaa gccaagggcc tcttgcggtc ccttcttact cacacacaaa atgtacccag
2641 tattctaggt agtgccctat tttacaattg taaaactgag gcacgagcaa agtgaagaca
2701 ctggctcata ttcctgcagc ctggaggccg ggtgctcagg gctgacacgt ccaccccagt
2761 gcacccactc tgctttgact gagcagactg gtgagcagac tggtgggatc tgtgcccaga
2821 gacgggactg ggagggccca cttcagggtt ctcctctccc ctctaaggcc gaagaagggt
2881 ccttccctct ccccaagact tggtgtcctt tccctccact ccttcctgcc acctgctgct
2941 gctgctgctg ctaatcttca gggcactgct gctgccttta gtcgctgagg aaaaataaag
3001 acaaatgctg cgcccttaaa aaaaaaaaaa aaaaaaaaaa a
//