LOCUS BC013082 2323 bp mRNA linear HUM 19-JAN-2018
DEFINITION Homo sapiens seven in absentia homolog 2 (Drosophila), mRNA (cDNA
clone MGC:17169 IMAGE:4153095), complete cds.
ACCESSION BC013082
VERSION BC013082.3
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2323)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2323)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (27-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
REFERENCE 3 (bases 1 to 2323)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (19-JAN-2018) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK Sequence update by database staff to remove vector contamination
COMMENT On Jan 19, 2018 this sequence version replaced BC013082.2.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 19 Row: l Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 55925659.
FEATURES Location/Qualifiers
source 1..2323
/db_xref="H-InvDB:HIT000036115"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:17169 IMAGE:4153095"
/tissue_type="Brain, glioblastoma with EGFR amplification"
/clone_lib="NCI_CGAP_Brn64"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2323
/gene="SIAH2"
/gene_synonym="hSiah2"
/db_xref="GeneID:6478"
/db_xref="HGNC:HGNC:10858"
/db_xref="MIM:602213"
CDS 306..1280
/gene="SIAH2"
/gene_synonym="hSiah2"
/codon_start=1
/product="seven in absentia homolog 2 (Drosophila)"
/protein_id="AAH13082.1"
/db_xref="GeneID:6478"
/db_xref="HGNC:HGNC:10858"
/db_xref="MIM:602213"
/translation="MSRPSSTGPSANKPCSKQPPPQPQHTPSPAAPPAAATISAAGPG
SSAVPAAAAVISGPGGGGGAGPVSPQHHELTSLFECPVCFDYVLPPILQCQAGHLVCN
QCRQKLSCCPTCRGALTPSIRNLAMEKVASAVLFPCKYATTGCSLTLHHTEKPEHEDI
CEYRPYSCPCPGASCKWQGSLEAVMSHLMHAHKSITTLQGEDIVFLATDINLPGAVDW
VMMQSCFGHHFMLVLEKQEKYEGHQQFFAIVLLIGTRKQAENFAYRLELNGNRRRLTW
EATPRSIHDGVAAAIMNSDCLVFDTAIAHLFADNGNLGINVTISTCCP"
BASE COUNT 504 a 629 c 612 g 578 t
ORIGIN
1 tgttccagaa gcgggtgggc cagggctctg cgcaccgctg gggttcgggg cccgggacgc
61 cgccgggagg agggcaccgc gcggggtccg acgcggaggc gtgctcggaa cgccgggggc
121 tgcggagtgc atcagcgcgg tccagccctc cgcctgccgg gcgccgagcg tctccgccgc
181 ccggacctgg gctgggcgcc gtggcgttgc ctcggagctc gctgcccgcg gggcgcgcac
241 cgccttgacc cgggcggccc cgcggcaggc aggcgcccgc agttccatgg ttggttcgga
301 gcgcgatgag ccgcccgtcc tccaccggcc ccagcgctaa taaaccctgc agcaagcagc
361 cgccgccgca gccccagcac actccgtccc cggctgcgcc cccggccgcc gccaccatct
421 cggctgcggg ccccggctcg tccgcggtgc ccgccgcggc ggcggtgatc tcgggccccg
481 gcggcggcgg cggggccggc ccggtgtccc cgcagcacca cgagctgacc tcgctcttcg
541 agtgtccggt ctgctttgac tatgtcctgc ctcctattct gcagtgccag gccgggcacc
601 tggtgtgtaa ccaatgccgc cagaagttga gctgctgccc gacgtgcagg ggcgccctga
661 cgcccagcat caggaacctg gctatggaga aggtggcctc ggcagtcctg tttccctgta
721 agtatgccac cacgggctgt tccctgaccc tgcaccatac ggagaaacca gaacatgaag
781 acatatgtga ataccgtccc tactcctgcc catgtcctgg tgcttcctgc aagtggcagg
841 ggtccctgga agctgtgatg tcccatctca tgcacgccca caagagcatt accacccttc
901 agggagaaga catcgtcttt ctagctacag acattaactt gccaggggct gtcgactggg
961 tgatgatgca gtcatgtttt ggccatcact tcatgctggt gctggagaaa caagagaagt
1021 acgaaggcca ccagcagttt tttgccatcg tcctgctcat tggcacccgc aagcaagccg
1081 agaactttgc ctacagactg gagttgaatg ggaaccggcg gagattgacc tgggaggcca
1141 cgccccgttc gattcatgac ggtgtggctg cggccatcat gaacagcgac tgccttgttt
1201 tcgacacagc catagcacat ctttttgcag ataatgggaa ccttggaatc aatgttacta
1261 tttctacatg ttgtccatga tgtgactttc gtaaaccttc aaaattattt gggcatagtg
1321 ctctatgttt aataaaggtt tttatagatg ttttattcca tatgtcttca caagtcagga
1381 cccacaatta cccgtgtttt gtttgaacag cagtgtccca tctggcttcg acccaacaaa
1441 gttcattaac ctgggatgaa tggggttggc ctgttggtga tttggatgct gttctgtgat
1501 ctaaaacaac tcttattgaa ttgtatttac tccctaaaca acacttgaca ggctgttgca
1561 cagggcttct ataagatcag tgtgttagga atgggaggcc ccttcctgcc tgccttccca
1621 tattggtccc ttgacattga caaaagcaca gtgactgtca gcagattcct ttacttttgt
1681 ttgtgggagg taggaattgt tttaatgcat tttaaacagt gtttctgaaa ttggatggct
1741 ggctaataga cactgaatca cccggagtgc ttatcttaaa attgcagatt tagggagcct
1801 gccaatttaa cagtctcatc aggtgattct tttcaacagt aatgtttgag aattactggg
1861 ttaaattgtg ggaaagggtc cagattttaa aggtgcttta aggttgccct ctgccgatac
1921 tgtttgtctt tctactgttt catcccctaa cttcccccaa ccctcaaatt aaaactagaa
1981 ctatagatcc acatgaacgc acgcctgaga tttggccact cacctatgtt ttgggtggat
2041 tgcctaggaa agcaagtcat atggccattg atagttctca tgtaattagt tttgctcacc
2101 actagtacag atgacccgtt tacacgtggc ttccctcgga agccctcctc aacagtagct
2161 ggtgtgaaag actaaatcag tagagttgga aaagctttat aaccggtgtg tcatatgctt
2221 gctatttaaa gctgtgtgtt ggttttgttt ttctgccaca ttcactagtt ttttaataaa
2281 tattttccaa aaatgaaaaa aaaaaaaaaa aaaaaaaaaa aaa
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