LOCUS BC013031 3336 bp mRNA linear HUM 27-JAN-2004
DEFINITION Homo sapiens pleckstrin homology-like domain, family B, member 1,
mRNA (cDNA clone IMAGE:3534425), partial cds.
ACCESSION BC013031
VERSION BC013031.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3336)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3336)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (20-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC013031.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: c Column: 2
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..3336
/db_xref="H-InvDB:HIT000036089"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3534425"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..3336
/gene="PHLDB1"
/gene_synonym="FLJ00141"
/gene_synonym="KIAA0638"
/gene_synonym="LL5A"
/db_xref="GeneID:23187"
CDS <1..2076
/gene="PHLDB1"
/gene_synonym="FLJ00141"
/gene_synonym="KIAA0638"
/gene_synonym="LL5A"
/codon_start=1
/product="PHLDB1 protein"
/protein_id="AAH13031.2"
/db_xref="GeneID:23187"
/translation="GGPEAGELPSIGEATAALALAGRRPSRGLAGASGRSSEEPGVAT
QRLWESMERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGHVEQL
KVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVALETGIQ
KERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRSIAKRKE
RLAILDSQAGQIRAQAVQESERLARDKNASLQLLQKEKEKLTVLERRYHSLTGGRPFP
KTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSAL
LTQNGTGSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQW
DALHGAAPFPAGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSIF
TGGNSACSPDNMSSASGLDMGKIEEMEKMLKEAHAEKNRLMESREREMELRRQALEEE
RRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIES
SGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLK
GVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTG
AEGYTQFMN"
misc_feature 238..519
/gene="PHLDB1"
/gene_synonym="FLJ00141"
/gene_synonym="KIAA0638"
/gene_synonym="LL5A"
/note="PspA_IM30; Region: PspA/IM30 family. This family
includes PspA a protein that suppresses sigma54-dependent
transcription. The PspA protein, a negative regulator of
the Escherichia coli phage shock psp operon, is produced
when virulence factors are exported through secretins in
many Gram-negative pathogenic bacteria and its homologue
in plants, VIPP1, plays a critical role in thylakoid
biogenesis, essential for photosynthesis. Activation of
transcription by the enhancer-dependent bacterial
sigma(54) containing RNA polymerase occurs through ATP
hydrolysis-driven protein conformational changes enabled
by activator proteins that belong to the large AAA(+)
mechanochemical protein family. It has been shown that
PspA directly and specifically acts upon and binds to the
AAA(+) domain of the PspF transcription activator"
/db_xref="CDD:pfam04012"
misc_feature 1747..2037
/gene="PHLDB1"
/gene_synonym="FLJ00141"
/gene_synonym="KIAA0638"
/gene_synonym="LL5A"
/note="PH; Region: PH domain. PH stands for pleckstrin
homology"
/db_xref="CDD:pfam00169"
BASE COUNT 788 a 926 c 1021 g 601 t
ORIGIN
1 gggggacctg aggctgggga gcttcccagc attggggagg ccaccgcagc attggcactg
61 gcaggccgga ggccctcacg aggccttgca ggggcctctg ggcggagcag cgaggagcct
121 ggcgttgcca cccaacgcct atgggagagt atggagcgct cagatgagga aaatctcaag
181 gaggagtgca gcagcactga gagcacccag caggagcacg aagatgcacc tagcaccaag
241 ctccagggag aggtgctagc cctggaagaa gagcgggctc aggtgctggg gcacgtggag
301 cagctcaagg tccgtgtgaa ggagctagag cagcagctgc aggagtcagc ccgagaggcc
361 gaaatggagc gggcactgct gcagggagag agggaggcag agcgggcact gctgcagaag
421 gagcagaagg cagtggatca gctgcaggag aagctggtgg ccttggagac aggcatccag
481 aaggagaggg acaaggaggc agaggccctg gagactgaga caaagctctt tgaggacttg
541 gagttccagc agttggagcg ggagagccgc gtggaggagg agcgcgagct ggccggccag
601 gggctgctcc ggagcaaggc tgagctgctc cgcagcatcg ccaagaggaa ggagcgcctg
661 gccatcctgg acagtcaggc tgggcagatc cgggctcagg ccgtgcagga gtcagaacgc
721 ctggcccggg acaagaatgc ctccttacag ctgctgcaaa aggagaagga gaagctgact
781 gtgctggaaa ggagatacca ctcactcaca gggggcaggc ctttcccgaa gaccacatcg
841 accctcaaag aggtttaccg ctccaagatg gatggcgagg ccaccagccc ccttccccgg
901 acccgcagcg gccccctccc ctcctcctct ggctcttcct cctcctcctc ccagctcagc
961 gtggctaccc tggggcgtag cccctcccca aagagcgctc tactcaccca gaatggcacg
1021 ggcagccttc ctcgcaacct ggcagccaca ctgcaggaca tcgagaccaa gcgccaacta
1081 gctctgcagc agaagggaca acaagtgatt gaagagcagc ggcggcgact ggctgagctg
1141 aagcagaaag cggcagctga ggcacagtgc cagtgggatg cccttcacgg ggcagcaccc
1201 ttcccagcgg gcccctcggg cttcccccct ctcatgcacc actctatcct acaccacctg
1261 cctgcggggc gggagcgtgg ggaggagggt gagcacgcct atgatacgct gagtctggag
1321 agctctgaca gcatggagac cagcatcttc accgggggca actcggcctg ctcccctgac
1381 aacatgtcca gcgcgagtgg tctggacatg gggaagatcg aggagatgga gaagatgctg
1441 aaagaggctc atgcagagaa gaaccggctc atggagtcga gggagcggga gatggagctg
1501 cggcggcagg ccctggagga ggagcggcgg aggcgtgagc aggtagaacg gaggctgcag
1561 agtgagagtg cccggaggca gcagctggtc gagaaggagg tcaagatgcg ggagaaacaa
1621 ttttcccagg cacgacccct gacccgctac ctgccaatcc ggaaggagga ctttgacctg
1681 aagacacata ttgagtcatc gggccatggt gttgatacct gcctgcacgt ggtgctcagc
1741 agcaaggtct gccgtggcta cttggtcaag atgggcggca agattaaatc atggaagaag
1801 cgctggtttg tcttcgaccg gctcaagcgc accctttcct attatgtgga caagcatgag
1861 acgaagctga agggagtcat ctatttccag gccattgagg aagtgtacta cgaccacctg
1921 cgcagtgcag ccaagagccc gaacccagcc ctcaccttct gcgtaaagac ccatgaccgg
1981 ctgtactaca tggtggcccc atctgcagag gccatgcgta tctggatgga tgtcattgtc
2041 acaggggctg agggctacac tcagttcatg aactaactgc cgtgggcctc ctggcagagc
2101 acaactgggg cttttgtata agaagacttt aatattctgt aaggagcttg gtcctgtgag
2161 tttctgggct ctggcctcct gaagaaccag ccagaagaag aaaagtagag gtggctttgc
2221 tgcctcctgg gagcccagaa cttgcagtaa ccctttaggg tcctgcccca ggcccagcca
2281 gggctgagga gctgtcacag agagggcctc agctctgacc tgacacctgc tctccccagc
2341 ctgttttctc ttttctaaaa gacaaattat ggtaccataa gctgccaaag atcccctcct
2401 gcctcagacc cctttgccag gggctttggg ggctgagcag agccacatcc agagtggggt
2461 aatagctcag gcggcccgct tcccatttct caaaccccgc tctgccccat tgttctcctt
2521 tcccttatac tttttattac cttgctcaag ggccagagat ctcaagtgtc aaccttgagg
2581 tcccagctcc atcccctagt tgcagactca tcaccatggt taccatagtg actgcttcat
2641 tgccatggtt acatactaat tgctgcagct ctgtggccca gcccactgct tcagctgtgg
2701 gccatctgag ggtacgtgcc atcatctctc cagcccaggc ccctgggcat ctcatgctgg
2761 ggggaaggga ctgaatacct ttttccttcc ccctgcctgt gtcttcagcc ctgatgcaca
2821 ggctgccagc cccccagtcc agccctctcc cttccactgg tgccttgctt agagccagaa
2881 gggatgaagc cgggggatct atggaacaga ggaggagcga tgcagttggg agaggaagct
2941 agaagggtta tggttggagt tctgtacagt gttgagtttc cgacagggaa agaggattcc
3001 tccaatgcgc ctagagagaa agcctgagca ggagatgatg cagcagaggg gaagggccct
3061 gtggtgccgc cgcccttcct tcagcctccg aagggtgatg gaaatggaga gtggaggacc
3121 aggcctccag ctgtctggcc tcgcccttca cgccttaaca ctaagcccac ctcccctgct
3181 ctccttccca gcattgagcc cttggttgcc tgggcccagg ctgggggttt tcagtatttg
3241 taagcatttc agcagaacaa taaagccttt ggactacgaa aaaaaaaaaa aaaaaaaaaa
3301 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa
//