LOCUS BC012616 2500 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens betaine-homocysteine methyltransferase, mRNA (cDNA
clone MGC:13645 IMAGE:4045543), complete cds.
ACCESSION BC012616
VERSION BC012616.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2500)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2500)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 19 Row: b Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4502406.
FEATURES Location/Qualifiers
source 1..2500
/db_xref="H-InvDB:HIT000035904"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:13645 IMAGE:4045543"
/tissue_type="Kidney"
/clone_lib="NIH_MGC_75"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..2500
/gene="BHMT"
/db_xref="GeneID:635"
/db_xref="HGNC:HGNC:1047"
/db_xref="MIM:602888"
CDS 82..1302
/gene="BHMT"
/codon_start=1
/product="betaine-homocysteine methyltransferase"
/protein_id="AAH12616.1"
/db_xref="GeneID:635"
/db_xref="HGNC:HGNC:1047"
/db_xref="MIM:602888"
/translation="MPPVGGKKAKKGILERLNAGEIVIGDGGFVFALEKRGYVKAGPW
TPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYASEDKLENRGNYVLEKISGQEVNEA
ACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKVFLQQLEVFMKKNVDFLIAEY
FEHVEEAVWAVETLIASGKPVAATMCIGPEGDLHGVPPGECAVRLVKAGASIIGVNCH
FDPTISLKTVKLMKEGLEAARLKAHLMSQPLAYHTPDCNKQGFIDLPEFPFGLEPRVA
TRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSG
LDMHTKPWVRARARKEYWENLRIASGRPYNPSMSKPDGWGVTKGTAELMQQKEATTEQ
QLKELFEKQKFKSQ"
BASE COUNT 783 a 515 c 594 g 608 t
ORIGIN
1 ggggagctcg gggctgcgca gcgggaaggc tcgcctagtc ggtccgcatc cgtgtcgacc
61 acctgtctgg acaccacgaa gatgccaccc gttgggggca aaaaggccaa gaagggcatc
121 ctagaacgtt taaatgctgg agagattgtg attggagatg gagggtttgt ctttgcactg
181 gagaagaggg gctacgtaaa ggcaggaccc tggactcctg aagctgctgt ggagcaccca
241 gaagcagttc gccagcttca tcgagagttc ctcagagctg gctcaaacgt catgcagacc
301 ttcaccttct atgcgagtga agacaagctg gagaacaggg gcaactatgt cttagagaag
361 atatctgggc aggaagtcaa tgaagctgct tgcgacatcg cccgacaagt ggctgatgaa
421 ggagatgctt tggtagcagg aggagtgagt cagacacctt cataccttag ctgcaagagt
481 gaaactgaag tcaaaaaagt atttctgcaa cagttagagg tctttatgaa gaagaacgtg
541 gacttcttga ttgcagagta ttttgaacac gttgaagaag ctgtgtgggc agttgaaacc
601 ttgatagcat ccggtaaacc tgtggcagca accatgtgca ttggcccaga aggagatttg
661 catggcgtgc cccccggcga gtgtgcagtg cgcctggtga aagcaggagc atccatcatt
721 ggtgtgaact gccactttga ccccaccatt agtttaaaaa cagtgaagct catgaaggag
781 ggcttggagg ctgcccgact gaaagctcac ctgatgagcc agcccttggc ttaccacact
841 cctgactgca acaagcaggg attcatcgat ctcccagaat tcccatttgg actggaaccc
901 agagttgcca ccagatggga tattcaaaaa tacgccagag aggcctacaa cctgggggtc
961 aggtacattg gcgggtgctg tggatttgag ccctaccaca tcagggcaat tgcagaggag
1021 ctggccccag aaaggggctt tttgccacca gcttcagaaa aacatggcag ctggggaagt
1081 ggtttggaca tgcacaccaa accctgggtt agagcaaggg ccaggaagga atactgggag
1141 aatcttcgga tagcctcagg ccggccatac aacccttcaa tgtcaaagcc agatggctgg
1201 ggagtgacca aaggaacagc cgagctgatg cagcagaaag aagccacaac tgagcagcag
1261 ctgaaagagc tctttgaaaa acaaaaattc aaatcacagt agcctcgata gaagctattt
1321 ttgatgaatt tctaggtgtt tgggtcacag ttcctacaaa tacggaaaag ggggttaaaa
1381 agcagtgctt tcatgaatgc catcctacac atattattgc tattacctga acaaaataga
1441 attacaaata gcacttgata attttaaagt atgttttaga aattttctta ggagcaaaat
1501 aagtacaaag taaatcttga acaggttcac taagcaccca ccctgtgaaa agtattatgg
1561 aaatcactgc tgcacaggaa aagtaattca gatgttaatg ccacttgaag aagttggtag
1621 gctagcaaag aggatgagac atgaactgtc ataaaggact cagcaaccag ccagggacag
1681 ataaagcgct atggaaaggg gcttccaagt tcttttgaac atgaccctta gtaacaaaca
1741 caatttatat aatgacccag caaaacacat cacatcttac tgtcgaaatt aaatgtgtga
1801 tccatcctag tattttctgt tccattcctt ttcattctat ttcatttata aaacatgcta
1861 gttgagactt ttcaaatgga tttttatgac ccactactgg gtttggatcc acagtttgaa
1921 aaatattgct acaagacact taaggagacc atcctgttta agtttattct tataagtagg
1981 tcagtcatat gagacctgat caataaatat ccaataccca gagtcctgct ctcagagttc
2041 ttctgtttcg tgacccactt ttctaccagt aaaagacata gaccaatggg gaggagggga
2101 ggagagatgg atatttcagc cctctccatc ctagtcaaca ctggatccac ctagtgcctc
2161 tgggccataa ggctgagcag agtgagcttg tattagttgg tagcttttaa aaaatataat
2221 aagaaaaaag tagagattct ccaaactcta gcctggtttc ctagattgag aactatgata
2281 tttttctctg ataatttaat atctactctc ctacaaaagc tcaagcctga agatacaaga
2341 ctattagaag aaacatgact accctcagtg tattagaaaa gaggtcatgc agctttctaa
2401 acattattga attgtttgag ctgttttgaa attgtaattc ttttcagcta ttaaaaagaa
2461 gagcaatgac aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
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