LOCUS BC012132 2494 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 1, mRNA (cDNA
clone MGC:20222 IMAGE:4549185), complete cds.
ACCESSION BC012132
VERSION BC012132.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2494)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2494)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP/Gazdar
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 29 Row: b Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..2494
/db_xref="H-InvDB:HIT000035681"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20222 IMAGE:4549185"
/tissue_type="Lung, large cell carcinoma"
/clone_lib="NIH_MGC_18"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2494
/gene="DDX1"
/gene_synonym="DBP-RB"
/gene_synonym="UKVH5d"
/db_xref="GeneID:1653"
/db_xref="HGNC:HGNC:2734"
/db_xref="MIM:601257"
CDS 50..2272
/gene="DDX1"
/gene_synonym="DBP-RB"
/gene_synonym="UKVH5d"
/codon_start=1
/product="DEAD (Asp-Glu-Ala-Asp) box polypeptide 1"
/protein_id="AAH12132.1"
/db_xref="GeneID:1653"
/db_xref="HGNC:HGNC:2734"
/db_xref="MIM:601257"
/translation="MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLM
AAETGSGKTGAFSIPVIQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAF
AIGSDGLCCQSREVKEWHGCRATKGLMKGKHYYEVSCHDQGLCRVGWSTMQASLDLGT
DKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEI
PPHMKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSGNAQ
VTQTKFLPNAPKALIVEPSRELAEQTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLS
VLENGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIP
QVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNP
KTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQ
AIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDV
RFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKE
KVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVD
EFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF
"
BASE COUNT 808 a 437 c 597 g 652 t
ORIGIN
1 gtcgggaggg agggagcgag caggcgaagc cgcggaggac ggggtgaaga tggcggcctt
61 ctccgagatg ggtgtaatgc ctgagattgc acaagctgtg gaagagatgg attggctcct
121 cccaactgat atccaggctg aatctatccc attgatctta ggaggaggtg atgtacttat
181 ggctgcagaa acaggaagtg gcaaaactgg tgcttttagt attccagtta tccagatagt
241 ttatgaaact ctgaaagacc aacaggaagg caaaaaagga aaaacaacaa ttaaaactgg
301 tgcttcagtg ctgaacaaat ggcagatgaa cccatatgac agaggatctg cttttgcaat
361 tgggtcagat ggtctttgtt gtcaaagcag agaagtaaag gaatggcatg ggtgtagagc
421 tactaaagga ttaatgaaag ggaaacacta ctatgaagta tcctgtcatg accaagggtt
481 atgcagggtc gggtggtcta ccatgcaggc ctctttggac ctaggtactg acaagtttgg
541 atttggcttt ggtggaacag gaaagaaatc ccataacaaa caatttgata attatggaga
601 ggaattcact atgcatgata ccattggatg ttacctggat atagataagg gacatgtcaa
661 gttctccaaa aatggaaaag atcttggtct ggcatttgaa ataccaccac atatgaaaaa
721 ccaagccctc tttcctgcct gtgttttgaa gaatgctgaa ctgaaattta acttcggtga
781 agaggaattt aagtttccac caaaagatgg ctttgttgct ctttccaagg caccggatgg
841 ttacattgtc aaatcacagc actcaggtaa tgcacaggtg acacaaacaa agtttctccc
901 caatgctccg aaagctctca ttgttgaacc ttcccgggag ttagctgaac aaactttgaa
961 taacatcaag cagtttaaga aatacattga taatcctaaa ttaagggagc ttctgataat
1021 tggaggtgtt gcagcccggg atcagctctc tgttttggaa aatggagtag atatagttgt
1081 aggtactccg ggaagactag atgacttggt gtcaactgga aagctgaact tatctcaagt
1141 tagattcctg gtcctggatg aagctgatgg gcttctttct caaggttatt ctgattttat
1201 aaataggatg cacaatcaga ttcctcaggt tacctctgat ggaaaaagac ttcaggtgat
1261 tgtttgctct gccactttgc attctttcga tgtaaagaaa ctgtccgaga agataatgca
1321 ttttcctaca tgggttgact taaaaggaga agactctgtt ccagatactg tacaccatgt
1381 tgttgtccca gtaaatccca aaactgacag actctgggaa aggcttggaa agagccacat
1441 tagaactgat gatgtacatg caaaagataa cacaagacct ggtgctaata gtccagagat
1501 gtggtctgaa gctattaaaa tcctgaaagg ggagtatgct gtccgggcaa tcaaggaaca
1561 taagatggat caagcaatta tcttctgtag aaccaaaatt gactgtgata acttggagca
1621 gtactttata caacaaggag gaggacctga taaaaaagga caccagttct catgtgtttg
1681 tcttcatggt gacagaaagc ctcatgagag aaagcaaaac ttggaaagat ttaagaaagg
1741 agatgtaaga ttcttgattt gcacagatgt agctgctaga ggaattgata tccacggtgt
1801 tccttatgtt ataaatgtca ctctgcccga tgaaaagcaa aactacgtac atcgaattgg
1861 cagagtagga agagctgaaa ggatgggtct ggcaatttcc ctggtggcaa cagaaaaaga
1921 aaaggtttgg taccatgtat gtagcagccg tggaaaaggg tgttataaca caagactcaa
1981 ggaagatgga ggctgtacca tatggtacaa cgagatgcag ttactatctg agatagaaga
2041 acacctgaac tgtaccattt ctcaggttga gccggatata aaggtaccag tggatgaatt
2101 tgatgggaaa gttacctacg gtcagaaaag ggctgctggt ggtggaagct ataaaggcca
2161 tgtggatatt ttggcaccta ctgttcaaga gttggctgcc cttgaaaagg aggcgcagac
2221 atctttcctg catcttggct accttcctaa ccagctgttc agaaccttct gatttttaca
2281 tttactgaat aagatttgag taatgaaagt ctgtagtctt aaaactctaa aacagttgta
2341 ctgcttccaa gcagcagtat ttatagtaac gtaagctatt aatgctaact cttgcatgtc
2401 aagaaacatt agtcttagga attcttcaaa aaatggcatc ccaatgaaaa taaatttgat
2461 gactataaaa aaaaaaaaaa aaaaaaaaaa aaaa
//