LOCUS BC011852 2773 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens glutamate-ammonia ligase (glutamine synthetase), mRNA
(cDNA clone MGC:20322 IMAGE:4137547), complete cds.
ACCESSION BC011852
VERSION BC011852.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2773)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2773)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (30-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC011852.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 28 Row: k Column: 11
This clone was selected for full length sequencing because it
passed the following selection criteria: Similarity but not
identity to protein.
FEATURES Location/Qualifiers
source 1..2773
/db_xref="H-InvDB:HIT000035492"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:20322 IMAGE:4137547"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2773
/gene="GLUL"
/gene_synonym="GS"
/gene_synonym="PIG43"
/db_xref="GeneID:2752"
/db_xref="HGNC:HGNC:4341"
/db_xref="MIM:138290"
CDS 121..1242
/gene="GLUL"
/gene_synonym="GS"
/gene_synonym="PIG43"
/codon_start=1
/product="glutamate-ammonia ligase (glutamine synthetase)"
/protein_id="AAH11852.1"
/db_xref="GeneID:2752"
/db_xref="HGNC:HGNC:4341"
/db_xref="MIM:138290"
/translation="MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKT
RTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVF
KYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGP
YYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHL
WVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEK
LSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKK
GYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN"
BASE COUNT 716 a 619 c 680 g 758 t
ORIGIN
1 cgagagtggg agaagagcgg agcgtgtgag cagtactgcg gcctcctctc ctctcctaac
61 ctcgctctcg cggcctacct ttacccgccc gcctgctcgg cgaccagaac accttccacc
121 atgaccacct cagcaagttc ccacttaaat aaaggcatca agcaggtgta catgtccctg
181 cctcagggtg agaaagtcca ggccatgtat atctggatcg atggtactgg agaaggactg
241 cgctgcaaga cccggaccct ggacagtgag cccaagtgtg tggaagagtt gcctgagtgg
301 aatttcgatg gctctagtac tttacagtct gagggttcca acagtgacat gtatctcgtg
361 cctgctgcca tgtttcggga ccccttccgt aaggacccta acaagctggt gttatgtgaa
421 gttttcaagt acaatcgaag gcctgcagag accaatttga ggcacacctg taaacggata
481 atggacatgg tgagcaacca gcacccctgg tttggcatgg agcaggagta taccctcatg
541 gggacagatg ggcacccctt tggttggcct tccaacggct tcccagggcc ccagggtcca
601 tattactgtg gtgtgggagc agacagagcc tatggcaggg acatcgtgga ggcccattac
661 cgggcctgct tgtatgctgg agtcaagatt gcggggacta atgccgaggt catgcctgcc
721 cagtgggaat ttcagattgg accttgtgaa ggaatcagca tgggagatca tctctgggtg
781 gcccgtttca tcttgcatcg tgtgtgtgaa gactttggag tgatagcaac ctttgatcct
841 aagcccattc ctgggaactg gaatggtgca ggctgccata ccaacttcag caccaaggcc
901 atgcgggagg agaatggtct gaagtacatc gaggaggcca ttgagaaact aagcaagcgg
961 caccagtacc acatccgtgc ctatgatccc aagggaggcc tggacaatgc ccgacgtcta
1021 actggattcc atgaaacctc caacatcaac gacttttctg ctggtgtagc caatcgtagc
1081 gccagcatac gcattccccg gactgttggc caggagaaga agggttactt tgaagatcgt
1141 cgcccctctg ccaactgcga ccccttttcg gtgacagaag ccctcatccg cacgtgtctt
1201 ctcaatgaaa ccggcgatga gcccttccag tacaaaaatt aagtggacta gacctccagc
1261 tgttgagccc ctcctagttc ttcatcccac tccaactctt ccccctctcc cagttgtccc
1321 gattgtaact caaagggtgg aatatcaagg tcgttttttt cattccatgt gcccagttaa
1381 tcttgctttc tttgtttggc tgggatagag gggtcaagtt attaatttct tcacacctac
1441 cctccttttt ttccctatca ctgaagcttt ttagtgcatt agtggggagg agggtgggga
1501 gacataacca ctgcttccat ttaatggggt gcacctgtcc aataggcgta gctatccgga
1561 cagagcacgt ttgcagaagg gggtctcttc ttccaggtag ctgaaagggg aagacctgac
1621 gtactctggt taggttagga cttgccctcg tggtggaaac ttttcttaaa aagttataac
1681 caacttttct attaaaagtg ggaattagga gagaaggtag gggttgggaa tcagagagaa
1741 tggctttggt ctcttgcttg tgggactagc ctggcttggg actaaatgcc ctgctctgaa
1801 cacgaagctt agtataaact gatggatatc cctaccttga aagaagaaaa ggttcttact
1861 gcttggtcct tgatttatca cacaaagcag aatagtattt ttatatttaa atgtaaagac
1921 aaaaaactat atgtatggtt ttgtggatta tgtgtgtttt gctaaaggaa aaaaaccatc
1981 caggtcacgg ggcaccaaat ttgagacaaa tagtcggatt agaaataaag catctcattt
2041 tgagtagaga gcaagggaag tggttcttag atggtgatct gggattaggc cctcaagacc
2101 cttttgggtt tctgccctgc ccaccctctg gagaaggtgg gcactggatt agttaacaga
2161 caacacgtta ctagcagtca cttgatctcc gtggctttgg tttaaaagac acacttgtcc
2221 acataggttt agagataaga gttggctggt caacttgagc atgttactga cagagggggt
2281 attggggtta ttttctggta ggaatagcat gtcactaaag caggcctttt gatattaaat
2341 tttttaaaaa gcaaaattat agaagtttag attttaatca aatttgtagg gtttctaggt
2401 aatttttaca gaattgcttg tttgcttcaa ctgtctccta cctctgctct tggaggagat
2461 ggggacaggg ctggagtcaa aacacttgta attttgtatc ttgatgtctt tgttaagact
2521 gctgaagaat tatttttttt cttttataat aaggaataaa ccccaccttt attccttcat
2581 ttcatctacc attttctggt tcttgtgttg gctgtggcag gccagctgtg gttttctttt
2641 gccatgacaa cttctaattg ccatgtacag tatgttcaaa gtcaaataac tcctcattgt
2701 aaacaaactg tgtaactgcc caaagcagca cttataaatc agcctaacat aaaaaaaaaa
2761 aaaaaaaaaa aaa
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