LOCUS BC011726 3112 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens Rho guanine nucleotide exchange factor (GEF) 1, mRNA
(cDNA clone MGC:19628 IMAGE:4138058), complete cds.
ACCESSION BC011726
VERSION BC011726.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3112)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3112)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (30-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC011726.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 27 Row: n Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..3112
/db_xref="H-InvDB:HIT000035404"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:19628 IMAGE:4138058"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3112
/gene="ARHGEF1"
/gene_synonym="GEF1"
/gene_synonym="LBCL2"
/gene_synonym="P115-RHOGEF"
/gene_synonym="SUB1.5"
/db_xref="GeneID:9138"
/db_xref="HGNC:HGNC:681"
/db_xref="MIM:601855"
CDS 85..2724
/gene="ARHGEF1"
/gene_synonym="GEF1"
/gene_synonym="LBCL2"
/gene_synonym="P115-RHOGEF"
/gene_synonym="SUB1.5"
/codon_start=1
/product="Rho guanine nucleotide exchange factor (GEF) 1"
/protein_id="AAH11726.1"
/db_xref="GeneID:9138"
/db_xref="HGNC:HGNC:681"
/db_xref="MIM:601855"
/translation="MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNS
QFQSLEQVKRRPAHLMALLQHVALQFEPGPLVLRVPVPPNVAFELDRTRADLISEDVQ
RRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHV
AERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMG
NRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPS
RDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTD
EGAETESPEPGDEGEPGRSGLELEPEEPPGWRELVPPDTLHSLPKSQVKRQEVISELL
VTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFLDRLMKRRQ
ESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQ
EAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVELAAECCREIL
HHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWR
VTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKTMLRPVLRLTSAMT
REVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALITETAGSLKVPAPASRPKP
RPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLLPFCRPGPEGQLAATALRKV
LSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEE
EFCRLRPLLSQLGGNSVPQPGCT"
BASE COUNT 643 a 976 c 991 g 502 t
ORIGIN
1 ccagggcccg ggatcgccga gcccgacctc gggcgccccg ccggtcacct ccgcgcggac
61 accagccctg cagagcccag ggagatggaa gacttcgccc gaggggcggc ctccccaggc
121 ccctcccggc ctggcctggt tcccgtcagc atcatcgggg ctgaggatga ggattttgag
181 aacgagctgg agacaaactc agaagagcaa aacagccagt tccagagcct ggagcaggtg
241 aagcggcgcc cagcccacct catggccctc ctgcagcacg tggccctgca gtttgagcca
301 ggacccctgg ttctccgggt gccggtccct cccaacgtcg cctttgaact tgaccgcact
361 agggctgacc tcatctccga ggatgtccag cggcggttcg tgcaggaggt ggtgcaaagc
421 cagcaggtag ccgtgggccg gcagctggag gacttccgtt ccaagcggct catgggcatg
481 acgccctggg agcaggagct ggcccagctg gaggcttggg ttgggcggga ccgagccagc
541 tacgaggccc gggagcggca cgtggcggag cggctgctca tgcacctgga ggagatgcaa
601 cataccatct ctaccgacga agaaaagagt gctgccgtgg tcaacgccat tggcctgtac
661 atgcgccacc ttggggtgcg gaccaagagt ggagacaaga agtcggggag gaacttcttc
721 cggaaaaagg tgatggggaa ccggcggtcg gacgagcctg ccaagaccaa gaaggggctg
781 agcagcatcc tggatgccgc ccgctggaac cggggagagc cccaggttcc agattttcga
841 cacctcaaag cagaggttga tgccgagaag ccaggtgcta cagaccggaa gggaggcgtg
901 gggatgccct ctcgggaccg gaatatcggg gctcctgggc aggacacccc tggagtctct
961 ctgcaccctc tgtccctgga cagcccagac cgggaaccag gtgctgacgc ccccctggag
1021 ctgggggact catccccgca gggcccaatg agcctggagt ccttggcgcc cccagagagt
1081 accgacgagg gggccgaaac cgagagcccc gagcctggag atgaggggga gccggggcgg
1141 tcgggactgg agcttgaacc agaagagcct cccggctggc gggaactcgt ccccccagac
1201 accctgcaca gcctgcccaa gagccaggtg aagcggcagg aggtcatcag cgagctgctg
1261 gtgacagagg cggcccacgt gcgcatgctg cgggtgctgc acgacctctt cttccagccc
1321 atggcagaat gcctgttctt ccccttggag gagctgcaga acatcttccc cagcctggac
1381 gagctcatcg aggtgcattc cctgttcctc gatcgcctga tgaagcggag gcaggagagt
1441 ggctacctca tcgaggagat cggagacgtg ctgctggccc ggtttgatgg tgctgagggc
1501 tcctggttcc agaaaatctc ctcccgcttc tgcagccgcc agtcatttgc cttagagcag
1561 ctcaaagcca agcaacgcaa ggaccctcgg ttctgtgcct tcgtgcagga agctgagagc
1621 cgcccgcggt gccgccgcct gcagctgaag gacatgatcc ccacggagat gcagcggctg
1681 accaagtacc ccctgctcct gcagagcatc gggcagaaca cagaagagcc cacagaacgg
1741 gagaaagtgg agctggcagc cgagtgctgc cgggaaattc tacaccacgt caaccaagcc
1801 gtgcgtgaca tggaggacct gctgaggctc aaggactatc agcggcgcct ggacttgtcc
1861 caccttcggc agagcagcga ccctatgctg agcgagttca agaacctgga catcaccaag
1921 aagaaattgg tccacgaggg cccactgacg tggcgggtga ctaaggacaa ggcagtggag
1981 gtgcatgtgc tgctgctgga cgacctgctg ctgctgctcc agcgccagga cgagcggctg
2041 ctgctcaagt cccatagccg gacactgacg cccacgcccg atggcaagac catgctgcgg
2101 cccgtgctgc ggctcacctc cgccatgacc cgcgaggtgg ccaccgatca caaagccttc
2161 tacgtccttt ttacctggga ccaggaggcc cagatatacg agctggtggc acagactgtg
2221 tcggagcgga aaaactggtg tgctctcatc actgagactg ccggatccct gaaagtccct
2281 gcccctgcct ctcgccctaa gccccggccc agcccgagca gcacccgaga acccctcctc
2341 agcagctctg agaacggcaa tggtggccga gagacgtctc cagctgatgc ccggaccgag
2401 agaatcctca gtgacctcct gcccttctgc agaccaggcc ccgagggcca gctcgctgcc
2461 acggcccttc ggaaagtgct gtccctgaag cagcttctgt ttccggcgga ggaagacaat
2521 ggggcggggc ctcctcgaga tggggatggg gtcccagggg gcggccccct gagcccagca
2581 cggacccagg aaatccagga gaacctgctc agcttggagg agaccatgaa gcagctggag
2641 gagttggagg aggaattttg ccgcctgaga cccctcctgt ctcagcttgg ggggaactct
2701 gtcccccagc ctggctgcac ttgaggttcc cgcccaggaa ggccttttgc aagaaggaga
2761 ggaatggggg agaggacgtg agggaccacc cccacccaca cagctgccgc agcatctcac
2821 accccgaggg cctgaggaga gggagctgtg ggccacgcct gggaggggcc cagctggggt
2881 tactggcccc gcatgagcct cggccatctc tccctcctgc cctctgcttg ggggactcag
2941 ggctccattc tggagggcac cacggtgacc cgggccatct cagtattgcc tgtgggggcc
3001 acccctccac ccccaccccc aagtgccttc gctctgtttt tataccctga attggaggtt
3061 tattttttaa tatatattat ctaagaagaa aaaaaaaaaa aaaaaaaaaa aa
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