LOCUS BC011700 2778 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens glutamate-ammonia ligase (glutamine synthetase), mRNA
(cDNA clone MGC:19700 IMAGE:3528830), complete cds.
ACCESSION BC011700
VERSION BC011700.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2778)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2778)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (30-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC011700.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 27 Row: d Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: Similarity but not
identity to protein.
FEATURES Location/Qualifiers
source 1..2778
/db_xref="H-InvDB:HIT000035384"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:19700 IMAGE:3528830"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2778
/gene="GLUL"
/gene_synonym="GS"
/gene_synonym="PIG43"
/db_xref="GeneID:2752"
/db_xref="HGNC:HGNC:4341"
/db_xref="MIM:138290"
CDS 110..1231
/gene="GLUL"
/gene_synonym="GS"
/gene_synonym="PIG43"
/codon_start=1
/product="glutamate-ammonia ligase (glutamine synthetase)"
/protein_id="AAH11700.1"
/db_xref="GeneID:2752"
/db_xref="HGNC:HGNC:4341"
/db_xref="MIM:138290"
/translation="MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKT
RTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVF
KYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGP
YYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHL
WVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEK
LSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKK
GYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN"
BASE COUNT 729 a 619 c 676 g 754 t
ORIGIN
1 gaagagcgga gcgtgtgagc agtactgcgg cctcctctcc tctcctaacc tcgctctcgc
61 ggcctacctt tacccgcccg cctgctcggc gaccagaaca ccttccacca tgaccacctc
121 agcaagttcc cacttaaata aaggcatcaa gcaggtgtac atgtccctgc ctcagggtga
181 gaaagtccag gccatgtata tctggatcga tggtactgga gaaggactgc gctgcaagac
241 ccggaccctg gacagtgagc ccaagtgtgt ggaagagttg cctgagtgga atttcgatgg
301 ctccagtact ttacagtctg agggttccaa cagtgacatg tatctcgtgc ctgctgccat
361 gtttcgggac cccttccgta aggaccctaa caagctggtg ttatgtgaag ttttcaagta
421 caatcgaagg cctgcagaga ccaatttgag gcacacctgt aaacggataa tggacatggt
481 gagcaaccag cacccctggt ttggcatgga gcaggagtat accctcatgg ggacagatgg
541 gcaccccttt ggttggcctt ccaacggctt cccagggccc cagggtccat attactgtgg
601 tgtgggagca gacagagcct atggcaggga catcgtggag gcccattacc gggcctgctt
661 gtatgctgga gtcaagattg cggggactaa tgccgaggtc atgcctgccc agtgggaatt
721 tcagattgga ccttgtgaag gaatcagcat gggagatcat ctctgggtgg cccgtttcat
781 cttgcatcgt gtgtgtgaag actttggagt gatagcaacc tttgatccta agcccattcc
841 tgggaactgg aatggtgcag gctgccatac caacttcagc accaaggcca tgcgggagga
901 gaatggtctg aagtacatcg aggaggccat tgagaaacta agcaagcggc accagtacca
961 catccgtgcc tatgatccca agggaggcct ggacaatgcc cgacgtctaa ctggattcca
1021 tgaaacctcc aacatcaacg acttttctgc tggtgtagcc aatcgtagcg ccagcatacg
1081 cattccccgg actgttggcc aggagaagaa gggttacttt gaagatcgtc gcccctctgc
1141 caactgcgac cccttttcgg tgacagaagc cctcatccgc acgtgtcttc tcaatgaaac
1201 cggcgatgag cccttccagt acaaaaatta agtggactag acctccagct gttgagcccc
1261 tcctagttct tcatcccact ccaactcttc cccctctccc agttgtcccg attgtaactc
1321 aaagggtgga atatcaaggt cgtttttttc attccatgtg cccagttaat cttgctttct
1381 ttgtttggct gggatagagg ggtcaagtta ttaatttctt cacacctacc ctcctttttt
1441 tccctatcac tgaagctttt tagtgcatta gtggggagga gggtggggag acataaccac
1501 tgcttccatt taatggggtg cacctgtcca ataggcgtag ctatccggac agagcacgtt
1561 tgcagaaggg ggactcttct tccaggtagc tgaaagggga agacctgacg tactctggtt
1621 aggttaggac ttgccctcgt ggtggaaact tttcttaaaa agttataacc aacttttcta
1681 ttaaaagtgg gaattaggag agaaggtagg ggttgggaat cagagagaat ggctttggtc
1741 tcttgcttgt gggactagcc tggcttggga ctaaatgccc tgctctgaac acgaagctta
1801 gtataaactg atggatatcc ctaccttgaa agaagaaaag gttcttactg cttggtcctt
1861 gatttatcac acaaagcaga atagtatttt tatatttaaa tgtaaagaca aaaaactata
1921 tgtatggttt tgtggattat gtgtgttttg ctaaaggaaa aaaccatcca ggtcacgggg
1981 caccaaattt gagacaaata gtcggattag aaataaagca tctcattttg agtagagagc
2041 aagggaagtg gttcttagat ggtgatctgg gattaggccc tcaagaccct tttgggtttc
2101 tgccctgccc accctctgga gaaggtgggc actggattag ttaacagacg acacgttact
2161 agcagtcact tgatctccgt ggctttggtt taaaagacac acttgtccac ataggtttag
2221 agataagagt tggctggtca acttgagcat gttactgaca gagggggtat tggggttatt
2281 ttctggtagg aatagcatgt cactaaagca ggccttttga tattaaattt tttaaaaagc
2341 aaaattatag aagtttagat tttaatcaaa tttgtagggt ttctaggtaa tttttacaga
2401 attgcttgtt tgcttcaact gtctcctacc tctgctcttg gaggagatgg ggacagggct
2461 ggagtcaaaa cacttgtaat tttgtatctt gatgtctttg ttaagactgc tgaagaatta
2521 tttttttctt ttataataag gaataaaccc cacctttatt ccttcatttc atctaccatt
2581 ttctggttct tgtgttggct gtggcaggcc agctgtggtt ttcttttgcc atgacaactt
2641 ctaattgcca tgtacagtat gttcaaagtc aaataactcc tcattgtaaa caaactgtgt
2701 aactgcccaa agcagcactt ataaatcagc ctaacataag aaaaaaaaaa aaaaaaaaaa
2761 aaaaaaaaaa aaaaaaaa
//