LOCUS BC011617 4049 bp mRNA linear HUM 19-JUN-2006
DEFINITION Homo sapiens pyruvate carboxylase, mRNA (cDNA clone MGC:10528
IMAGE:3946441), complete cds.
ACCESSION BC011617
VERSION BC011617.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4049)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4049)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (30-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC011617.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 15 Row: p Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 11761614.
FEATURES Location/Qualifiers
source 1..4049
/db_xref="H-InvDB:HIT000035332"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:10528 IMAGE:3946441"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..4049
/gene="PC"
/gene_synonym="PCB"
/db_xref="GeneID:5091"
/db_xref="HGNC:HGNC:8636"
/db_xref="MIM:608786"
CDS 84..3620
/gene="PC"
/gene_synonym="PCB"
/codon_start=1
/product="pyruvate carboxylase"
/protein_id="AAH11617.1"
/db_xref="GeneID:5091"
/db_xref="HGNC:HGNC:8636"
/db_xref="MIM:608786"
/translation="MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVAN
RGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDI
IKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAI
AAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYT
RAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVE
IAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHT
VTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTG
RIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEF
RVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVM
VNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLM
DTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPW
RRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNY
LPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCI
KDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAA
DSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKS
GNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIV
GDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLP
RVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPL
DSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSIL
VKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKME
TVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE"
BASE COUNT 871 a 1228 c 1238 g 712 t
ORIGIN
1 cagcagcggt agaggcggcg gcgaggactg gcgacggcga ggagatagtg tctgccttct
61 ggagagctga ccaaacacta aggatgctga agttccgaac agtccatggg ggcctgaggc
121 tcctgggaat ccgccgaacc tccaccgccc ccgctgcctc cccaaatgtc cggcgcctgg
181 agtataagcc catcaagaaa gtcatggtgg ccaacagagg tgagattgcc atccgtgtgt
241 tccgggcctg cacggagctg ggcatccgca ccgtagccat ctactctgag caggacacgg
301 gccagatgca ccggcagaaa gcagatgaag cctatctcat cggccgcggc ctggcccccg
361 tgcaggccta cctgcacatc ccagacatca tcaaggtggc caaggagaac aacgtagatg
421 cagtgcaccc tggctacggg ttcctctctg agcgagcgga cttcgcccag gcctgccagg
481 atgcaggggt ccggtttatt gggccaagcc cagaagtggt ccgcaagatg ggagacaagg
541 tggaggcccg ggccatcgcc attgctgcgg gtgttcccgt tgtccctggc acagatgccc
601 ccatcacgtc cctgcatgag gcccacgagt tctccaacac ctacggcttc cccatcatct
661 tcaaggcggc ctatgggggt ggagggcgtg gcatgagggt ggtgcacagc tacgaggagc
721 tggaggagaa ttacacccgg gcctactcag aggctctggc cgcctttggg aatggggcgc
781 tgtttgtgga gaagttcatc gagaagccac ggcacatcga ggtgcagatc ttgggggacc
841 agtatgggaa catcctgcac ctgtacgagc gagactgctc catccagcgg cggcaccaga
901 aggtggtcga gattgccccc gccgcccacc tggacccgca gcttcggact cggctcacca
961 gcgactctgt gaaactcgct aaacaggtgg gctacgagaa cgcaggcacc gtggagttcc
1021 tggtggacag gcacggcaag cactacttca tcgaggtcaa ctcccgcctg caggtggagc
1081 acacggtcac agaggagatc accgacgtag acctggtcca tgctcagatc cacgtggctg
1141 agggcaggag cctacccgac ctgggcctgc ggcaggagaa catccgcatc aacgggtgtg
1201 ccatccagtg ccgggtcacc accgaggacc ccgcgcgcag cttccagccg gacaccggcc
1261 gcattgaggt gttccggagc ggagagggca tgggcatccg cctggataat gcttccgcct
1321 tccaaggagc cgtcatctcg ccccactacg actccctgct ggtcaaagtc attgcccacg
1381 gcaaagacca ccccacggcc gccaccaaga tgagcagggc ccttgcggag ttccgcgtcc
1441 gaggtgtgaa gaccaacatc gccttcctgc agaatgtgct caacaaccag cagttcctgg
1501 caggcactgt ggacacccag ttcatcgacg agaacccaga gctgttccag ctgcggcctg
1561 cacagaaccg ggcccaaaag ctgttgcact acctcggcca tgtcatggta aacggtccaa
1621 ccaccccgat tcccgtcaag gccagcccca gccccacgga ccccgttgtc cctgcagtgc
1681 ccataggccc gcccccggct ggtttcagag acatcctgct gcgagagggg cctgagggct
1741 ttgctcgagc tgtgcggaac cacccggggc tgctgctgat ggacacgacc ttcagggacg
1801 cccaccagtc actgctggcc actcgtgtgc gcacccacga tctcaaaaag atcgccccct
1861 atgttgccca caacttcagc aagctcttca gcatggagaa ctggggagga gccacgtttg
1921 acgtcgccat gcgcttcctg tatgagtgcc cctggcggcg gctgcaggag ctccgggagc
1981 tcatccccaa catccctttc cagatgctgc tgcggggggc caatgctgtg ggctacacca
2041 actacccaga caacgtggtc ttcaagttct gtgaagtggc caaagagaat ggcatggatg
2101 tcttccgtgt gtttgactcc ctcaactact tgcccaacat gctgctgggc atggaggcgg
2161 caggaagtgc cggaggcgtg gtggaggctg ccatctcata cacgggcgac gtggccgacc
2221 ccagccgcac caagtactca ctgcagtact acatgggctt ggccgaagag ctggtgcgag
2281 ctggcaccca catcctgtgc atcaaggaca tggccgggct gctgaagccc acggcctgca
2341 ccatgctggt cagctccctc cgggaccgct tccccgacct cccactgcac atccacaccc
2401 acgacacgtc aggggcaggc gtggcagcca tgctggcctg tgcccaggct ggagctgatg
2461 tggtggatgt ggcagctgat tccatgtctg ggatgacttc acagcccagc atgggggccc
2521 tggtggcctg taccagaggg actcccctgg acacagaggt gcccatggag cgcgtgtttg
2581 actacagtga gtactgggag ggggctcggg gactgtacgc ggccttcgac tgcacggcca
2641 ccatgaagtc tggcaactcg gacgtgtatg aaaatgagat cccagggggc cagtacacca
2701 acctgcactt ccaggcccac agcatggggc ttggctccaa gttcaaggag gtcaagaagg
2761 cctatgtgga ggccaaccag atgctgggcg atctcatcaa ggtgacgccc tcctccaaga
2821 tcgtggggga cctggcccag tttatggtgc agaatggatt gagccgggca gaggccgaag
2881 ctcaggcgga agagctgtcc tttccccgct ccgtggtgga gttcctgcag ggctacatcg
2941 gtgtccccca tggggggttc cccgaaccct ttcgctctaa ggtactgaag gacctgccaa
3001 gggtggaggg gcggcctgga gcctccctcc ctcccctgga tctgcaggca ctggagaagg
3061 agctggtaga ccggcatggg gaggaggtga cgccggaaga tgtgctctca gcagctatgt
3121 accccgatgt gtttgcccac ttcaaggact tcactgccac ctttggcccc ctggatagcc
3181 tgaatactcg cctcttcctg cagggaccca agatcgcaga ggagtttgag gtggagctgg
3241 agcggggcaa gacgctgcac atcaaagccc tggccgtgag cgacctgaac cgggccggcc
3301 agaggcaggt cttctttgag ctcaatgggc agctgcggtc catcttggtc aaggacaccc
3361 aggccatgaa ggagatgcac ttccacccca aggccctaaa ggacgtgaag ggccagatcg
3421 gggcgcccat gcctgggaag gtgatagaca tcaaagtggt ggcaggggcc aaggtggcca
3481 agggccagcc cctgtgtgtg ctcagtgcca tgaagatgga gactgtggtg acctcaccca
3541 tggagggtac tgtccgcaag gttcatgtga ccaaggacat gacactggaa ggtgacgacc
3601 tcatcctgga gatcgagtga tcttgcccca gaccggcagc ctggccatcc ccaagccttc
3661 aacagaagct gtgctgccac ggcaggccca ggccagccag tgcccgaggc caggaaggcc
3721 gggccgtgga ggtcctgtcc acagctggac aggagagaca ccgcctgcgg tggttcattc
3781 ctttcagcca tcgtcctttc ctccggcgga cagctgctta catgttcatc tcttgccaaa
3841 taagggtccc ctcctcactg gagactacaa gtggtgggtc aggtggtcct aggacccagg
3901 ggaggtttag gggtcctatc tcctggggga aggggagatc taagatgtcc caggtcctgg
3961 gaagtttact caataaagct ggctttcccc tgccaaaaaa aaaaaaaaaa aaaaaaaaaa
4021 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//